We are seeking an enthusiastic and motivated research technician to join the newly established laboratory of Dr. David McEwan at the University of Dundee to support an exciting project to study the role of immune cells in combating infection. The successful applicant will work as part of a multidisciplinary team interested in understanding immune cell regulation. In particular, we have a primary focus on the process of autophagy (meaning “Self-eating”) that is utilized by cells to destroy intracellular pathogens, remove damaged organelles and proteins aggregates. We are interested in how autophagy and the degradative lysosomal compartment in specialized immune cells, such as macrophages and neutrophils, is utilized by the host and manipulated by the pathogen to evade detection/destruction. We will use a variety of techniques including primary cell isolation and culture, CRISPR/Cas9 gene editing, biochemical assays and confocal imaging to gain an in-depth understanding of these processes.
This is a unique opportunity to gain experience in an exciting field of research that can help us gain a better understanding of host-pathogen interactions and how to combat infection.
Duties will include:
• Support, organize and ensure the practical aspects of the day-to-day running of the projects.
• Grow and maintain primary and immortalized cells in culture.
• Assist the researcher with experimental set-up, transfections and biochemical assay development for the project, including performing routine cloning of constructs and site-directed mutagenesis.
• Contributing to the development and optimization of core molecular cell biology and biochemistry techniques and assays to support the project and the laboratory
• Develop, maintain and curate the Lab’s reagent databases (plasmids, vectors, antibodies)
• Report directly to the Principal Investigator (Dr. McEwan)
Your qualifications, skills and experience:
• Applicants should have good A-level or equivalent Higher qualifications, or a degree in a relevant chemical or biological subject.
• Familiarity with basic laboratory techniques such as DNA and RNA extraction, PCR, gel electrophoresis and Western blots.
• Previous experience in mammalian cell culture.
• Excellent interpersonal and communication skills and computer proficiency.
• Good organizational and time management skills.
• Excellent communication skills to coordinate with lab members.
• Previous experience in primary cell isolation would be an advantage, but is not essential.
The successful candidate will be based within the School of Life Sciences at the University of Dundee, one of the top life science research institutes in Europe for Biological Sciences. Comprising about 100 research groups, the highest number of citations-per-paper for biological sciences in Europe and over £100 million of research income in 2013, the School is equipped with outstanding laboratory and technology facilities to conduct cutting-edge research.
The post is available immediately. The post is available for a fixed term period until March 2019 in the first instance.
Informal enquiries may be made to Dr. David McEwan, e-mail: firstname.lastname@example.org
How to apply:
The University of Dundee is a diverse community and is committed to equality of opportunity for all by providing a supportive, flexible and inclusive working environment. We have family friendly policies (including flexible working and childcare vouchers), staff groups for LGBT and BME; and prayer room facilities.
The University of Dundee has received the Athena SWAN Bronze Award (http://www.dundee.ac.uk/about/athenaswan/) which recognises the promotion of gender equality and has made further commitment to advancing inclusive culture which supports and encourages all under-represented groups.
The University of Dundee is a Scottish Registered Charity, No. SC015096.