University of Dundee

Techniques

  • Imaging Competition entry by Alistair Davies

Below is a list of some of the most common techniques performed in the Dundee Imaging Facility. For further information please browse the resource pages or contact us for further information

 

Light Microscopy

  • Confocal microscopy
  • Super resolution microscopy
  • Widefield Deconvolution microscopy
  • Laser micro-dissection
  • TIRF
  • Label free (SHG and THG) Imaging
  • Live cell imaging
  • Multi-photon microscopy
  • PALM
  • FCS/FCCS
  • FRET
  • Structured Illumination Microscopy
  • Frequency and Time domain FLIM
  • FRAP
  • Spinning Disk Microscopy

 

Electron Microscopy and Non-Optical Imaging

  • Transmission Electron Microscopy
  • Scanning Electron Microscopy 
  • Cryo-SEM
  • Cryo TEM
  • Atomic Force Microscopy

 

Data Analysis

  • Image Restoration
  • Image Stitching & Registration
  • Image Segmentation
  • 3D Image Analysis & Visualisation
  • Cell Tracking
  • Single Particle Tracking & Optical Flow
  • Colocalization
  • Quantitative Image Analysis & Statistics
  • Automation and High Throughput

 

Sample Preparation

  • Resin embedding
  • Ultrathin sectioning
  • Serial sectioning
  • Tokuyasu embedding
  • Cryosectioning
  • Immunolabelling
  • Plunge Freezing
  • High Pressure Freezing
  • Freeze substitution
  • Critical Point Drying
  • Freeze fracture
  • Metal coating
  • Negative stain
  • Photoconversion
  • Immunofluorescence
  • Cryostat sectioning
  • Vibrating microtome
  • H&E staining
  • Enzyme histochemistry
  • Live Cell sample preparation