University of Dundee

Light Microscopy

Supporting individual research projects and collaborative and translational research at the University of Dundee

Fluorescence microscopy offers a unique approach to the study of living and fixed cells because of its sensitivity, specificity and versatility. Fluorescence emitted from the biological sample can be simultaneously detected as both an image and as photometric data using the microscope, and is a vital tool for qualitative and quantitative studies on the function and structure of cells.

Technologies

School of Life Sciences

  • DeltaVision Widefield Deconvolution Microscopy
  • Laser Scanning Confocal and Multiphoton microscopy
  • Laser Micro- dissection
  • Total Internal Reflected Fluorescence Microscopy
  • Spinning Disk and Frequency Modulated
  • Time Correlated Single Photon Counting Fluorescence Lifetime Imaging systems

We cover a wide range of applications including multiwavelength 3D imaging of fixed material, live imaging of cells, tissues and embryos, FRAP, FRET and laser damage and laser ablation.

School of Medicine

  • 2 microscope suites including a pair of Leica SP5 confocal microscopes equipped for live and fixed sample imaging
  • A Zeiss 710 confocal microscope (first installation in the UK of the Zeiss InTune laser) The pulsed InTune laser is a continuously tunable visible laser, allowing for fluorescent lifetime (FLIM) measurements to be made of protein-protein interactions
  • Two Deltavision widefield deconvolution microscope systems (for timelapse imaging of live cells where sensitivity and speed are critical)

People

The Light Microscopy Facility is supportedby Dr Paul Appleton, Dr Bavishna Balagopal, Dr Alan Prescott, Mrs Alison Milne and Mr Calum Thomson, whose experience and dedication enables both the quality and quantity of imaging carried out in the facility each year.

These systems were used for over 40,000 hours in 2014, generating 50 TB of data and contributing to roughly 30% of the College’s published output each year.

Services

We deliver a continuous program of one-to-one training to meet the demands of the University’s challenging research goals. The facility also supports undergraduate and postgraduate training via formal workshops and seminars. We are also a focus for the University's public outreach projects.