University of Dundee

Dissecting the roles of the FAM83 family of proteins in cells and disease through exploitation and development of cutting-edge technologies

Some recent discoveries in our lab have established key roles for relatively uncharacterised PAWS1/FAM83G, and the FAM83 family of proteins to which PAWS1 belongs, in tissue homeostasis, development and disease. One of the major focus of the lab is to understand how PAWS1 and the FAM83 family function and are regulated in cells and how their malfunction manifests in human diseases. The prospective PhD projects in this area include: 1) Dissecting the molecular roles and regulation of PAWS1 in development and signal transduction. 2) Assigning the biochemical and molecular role(s) for the domain of unknown function, DUF1669, which links the members of the uncharacterised FAM83 family of proteins. Prospective PhD candidates will exploit cutting-edge technologies, including CRISPR/Cas9 genome editing, mass spectrometry, ChIP and RNA sequencing as well as transgenic mouse models, to establish the physiological roles and regulation of PAWS1 and other members of the FAM83 family. Advancing new technologies to address fundamental biological questions is another area of our interest. With this in mind, the third prospective PhD project aims to develop and test applications of the Affinity-directed PROtein Missile (AdPROM) system, which combines CRISPR/Cas9 and nanobodies to target endogenous proteins, for research into protein function and drug discovery.