The talk will be followed by refreshments in WTB Life Space, to which everyone is invited.
Since the first mass spectra of non-covalent protein complexes were reported, focusing on soluble complexes, it became possible to elucidate ligand binding properties and subsequently to define subunit interaction maps and topological models. A long-term goal has been to extend the approaches developed for soluble complexes to one of the most challenging biological targets - that of membrane proteins and their assemblies.
Recent discoveries have enabled delivery of membrane complexes from detergent micelles in solution. By maintaining interactions between membrane and cytoplasmic subunits in the gas phase, it is now possible to investigate the effects of lipids, nucleotides and drugs on intact membrane assemblies. These investigations reveal allosteric and synergistic effects of small molecule binding and expose the consequences of post-translational modifications. In my lecture I will present recent progress in the study of protein complexes, focusing particularly on complexes extracted from membranes, and present future prospects for mass spectrometry in structural biology.
About the lecture series:
The Peter Garland Lecture was set up in 1985 to mark the achievement of Dundee’s first Professor of Biochemistry in building up the Department into one of the strongest in the UK over the period 1970 to 1984. Fourteen Peter Garland lecturers have had, or subsequently went on to win, a Nobel Prize.