LSR Fortessa (Becton Dickinson):
The LSR Fortessa is our custom specification, high end digital instrument and is located on the second floor of the Wellcome Trust Biocentre (room 2L3-24).
The Fortessa is equipped with five laser light sources; 488nm, 640nm, 355nm, 405nm and 561nm, and up to 19 colours can be detected at any one time. The cytometer runs using PC based DIVA software and is equiped with full cross-beam compensation and pulse processing cabailities on all parameters.
FACS Canto (Becton Dickinson):
The FACS Canto is also located on the second floor of the Wellcome Trust Biocentre. Like the LSR Fortessa, the FACS Canto is a digital system and runs using the PC based DIVA software.
It has three excitation light sources: 405nm and 488nm solid state lasers and a 633nm laser. From these light sources, up to eight colours can be detected: 450nm and 510nm from the 405nm laser; 525nm, 575nm, 678nm and 785nm from the 488nm laser; and 660nm and 785nm from the 633nm laser. The cytometer is able to perform pulse processing on all parameters and also allows full inter-beam compensation, either during or post data acquisition.
Novocyte (Acea Biosciences):
The Novocyte is located on the second floor of the Wellcome Trust Biocentre (room 2L3-24).
It has three laser exciation sources: 488nm, 640nm and 405nm, and is able to detect 13 fluorescence parameters: five from 488nm, two from 640nm and six from 405nm. Optical filters can be changed to allow optimisation of detection of many different fluorophores.
The Novocyte is equiped with a calibrated syringe pump for sample uptake, which allows accurate counting of cells while performing analyses. It also has a universal loader which enables automated sample uptake from a number of different vessles, including racked tubes and 96 well plates.
The Sony MA900 is the latest addition to our instrument portfolio. It is equipped with four laser excitation sources: 488nm, 405nm, 640nm and 561nm. The addition of the 561nm laser enables us to sort cells expressing fluorescent protiens such as mCherry and mKate. The sorter is able to detect up to 12 fluorescent parameters and the changeable filter sets allows modification of the detectors to any combination of colours from any of the four lasers.
The MA900 is housed in a class 2 biosafety cabinet, enabling us to sort samples derived from primary human tissue and those transfected with non-replicating human viruses.
The instrument is able to sort cells at a rate of up to 10,000 cells per second (depending on cell type) and up to four different populations can be collected at any one time. Cells can be depositied into a variety of different vessles, including 384-well and 96-well plates for single cell cloning. Temperature controlled sample and collection chambers preserve sample quality.
For more detailed information on what is required to successfully sort cells, see Techniques.
The Flow Cytometry and Cell Sorting Facility provides a PC equipped with FlowJo software designated solely for data analysis. However, many groups who perform a lot of flow cytometry choose to purchase a 'seat' on our Flowjo site license to enable data analysis on their own computers. Numerous other software packages are available from various sources.