University of Dundee

Biological Chemistry and Drug Discovery 2014

Ali, M., Leung, K. F. and Field, M. C. The ancient small GTPase Rab21 functions in intermediate endocytic steps in trypanosomes. (2014) Eukaryot Cell. 13, 304-319;
doi. 10.1128/EC.00269-13;
PMID; 3910970, PMC24376004

Alsford, S., Currier, R. B., Guerra-Assuncao, J. A., Clark, T. G. and Horn, D. Cathepsin-L can resist lysis by human serum in Trypanosoma brucei brucei. (2014) PLoS Pathog. 10, e1004130;
doi. 10.1371/journal.ppat.1004130;
PMID; 4022737, PMC24830321

Arora, K., Ochoa-Montano, B., Tsang, P. S., Blundell, T. L., Dawes, S. S., Mizrahi, V., Bayliss, T., Mackenzie, C. J., Cleghorn, L. A., Ray, P. C., Wyatt, P. G., Uh, E., Lee, J., Barry, C. E., 3rd and Boshoff, H. I. Respiratory Flexibility in Response to Inhibition of Cytochrome c Oxidase in Mycobacterium tuberculosis. (2014) Antimicrob Agents Chemother. 58, 6962-6965;
doi. 10.1128/AAC.03486-14;

Baud, M. G., Lin-Shiao, E., Cardote, T., Tallant, C., Pschibul, A., Chan, K. H., Zengerle, M., Garcia, J. R., Kwan, T. T., Ferguson, F. M. and Ciulli, A. Chemical biology. A bump-and-hole approach to engineer controlled selectivity of BET bromodomain chemical probes. (2014) Science. 346, 638-641;
doi. 10.1126/science.1249830;

Besnard, J., Jones, P. S., Hopkins, A. L. and Pannifer, A. D. The Joint European Compound Library: boosting precompetitive research. (2014) Drug Discov Today;
doi. 10.1016/j.drudis.2014.08.014;

Brand, S., Norcross, N. R., Thompson, S., Harrison, J. R., Smith, V. C., Robinson, D. A., Torrie, L. S., McElroy, S. P., Hallyburton, I., Norval, S., Scullion, P., Stojanovski, L., Simeons, F. R., van Aalten, D., Frearson, J. A., Brenk, R., Fairlamb, A. H., Ferguson, M. A., Wyatt, P. G., Gilbert, I. H. and Read, K. D. Lead optimization of a pyrazole sulfonamide series of Trypanosoma brucei N-myristoyltransferase inhibitors: identification and evaluation of CNS penetrant compounds as potential treatments for stage 2 human African trypanosomiasis. (2014) J Med Chem. 57, 9855-9869;
doi. 10.1021/jm500809c;
PMID; 4269550, PMC25412409

Capes, A. S., Crossman, A., Urbaniak, M. D., Gilbert, S. H., Ferguson, M. A. and Gilbert, I. H. Probing the substrate specificity of Trypanosoma brucei GlcNAc-PI de-N-acetylase with synthetic substrate analogues. (2014) Org Biomol Chem. 12, 1919-1934;
doi. 10.1039/c3ob42164c;
PMID; 4184461

Cole, C., Kroboth, K., Schurch, N. J., Sandilands, A., Sherstnev, A., O'Regan, G. M., Watson, R. M., McLean, W. H., Barton, G. J., Irvine, A. D. and Brown, S. J. Filaggrin-stratified transcriptomic analysis of pediatric skin identifies mechanistic pathways in patients with atopic dermatitis. (2014) J Allergy Clin Immunol. 134, 82-91;
doi. 10.1016/j.jaci.2014.04.021;
PMID; 4090750, PMC24880632

Damerow, M., Rodrigues, J. A., Wu, D., Guther, M. L., Mehlert, A. and Ferguson, M. A. Identification and functional characterization of a highly divergent N-acetylglucosaminyltransferase I (TbGnTI) in Trypanosoma brucei. (2014) J Biol Chem. 289, 9328-9339;
doi. 10.1074/jbc.M114.555029;
PMID; 3979372, PMC24550396

De Rycker, M., Hallyburton, I., Thomas, J., Campbell, L., Wyllie, S., Joshi, D., Cameron, S., Gilbert, I. H., Wyatt, P. G., Frearson, J. A., Fairlamb, A. H. and Gray, D. W. Erratum for De Rycker et al., Comparison of a High-Throughput High-Content Intracellular Leishmania donovani Assay with an Axenic Amastigote Assay. (2014) Antimicrob Agents Chemother. 58, 7622;
doi. 10.1128/AAC.04457-14;

Devos, D. P., Graf, R. and Field, M. C. Evolution of the nucleus. (2014) Curr Opin Cell Biol. 28, 8-15;
doi. 10.1016/;
PMID; 4071446, PMC24508984

Dias, D. M. and Ciulli, A. NMR approaches in structure-based lead discovery: Recent developments and new frontiers for targeting multi-protein complexes. (2014) Prog Biophys Mol Biol;
doi. 10.1016/j.pbiomolbio.2014.08.012;

Dias, D. M., Van Molle, I., Baud, M. G., Galdeano, C., Geraldes, C. F. and Ciulli, A. Is NMR Fragment Screening Fine-Tuned to Assess Druggability of Protein-Protein Interactions? (2014) ACS Med Chem Lett. 5, 23-28;
doi. 10.1021/ml400296c;
PMID; 3891296, PMC24436777

Diaz-Saez, L., Srikannathasan, V., Zoltner, M. and Hunter, W. N. Structures of bacterial kynurenine formamidase reveal a crowded binuclear zinc catalytic site primed to generate a potent nucleophile. (2014) Biochem J. 462, 581-589;
doi. 10.1042/BJ20140511;

Ferguson, F. M., Dias, D. M., Rodrigues, J. P., Wienk, H., Boelens, R., Bonvin, A. M., Abell, C. and Ciulli, A. Binding Hotspots of BAZ2B Bromodomain: Histone Interaction Revealed by Solution NMR Driven Docking. (2014) Biochemistry. 53, 6706-6716;
doi. 10.1021/bi500909d;

Fiebig, T., Freiberger, F., Pinto, V., Romano, M. R., Black, A., Litschko, C., Bethe, A., Yashunsky, D., Adamo, R., Nikolaev, A., Berti, F. and Gerardy-Schahn, R. Molecular cloning and functional characterization of components of the capsule biosynthesis complex of Neisseria meningitidis serogroup A: toward in vitro vaccine production. (2014) J Biol Chem. 289, 19395-19407;
doi. 10.1074/jbc.M114.575142;
PMID; 4094051, PMC24849599

Field, M. C., Koreny, L. and Rout, M. P. Enriching the pore: splendid complexity from humble origins. (2014) Traffic. 15, 141-156;
doi. 10.1111/tra.12141;
PMID; 3906644, PMC24279500

Fleming, J. R., Sastry, L., Crozier, T. W., Napier, G. B., Sullivan, L. and Ferguson, M. A. Proteomic selection of immunodiagnostic antigens for Trypanosoma congolense. (2014) PLoS Negl Trop Dis. 8, e2936;
doi. 10.1371/journal.pntd.0002936;
PMID; 4055490, PMC24922510

Galdeano, C., Gadd, M. S., Soares, P., Scaffidi, S., Van Molle, I., Birced, I., Hewitt, S., Dias, D. M. and Ciulli, A. Structure-Guided Design and Optimization of Small Molecules Targeting the Protein-Protein Interaction between the von Hippel-Lindau (VHL) E3 Ubiquitin Ligase and the Hypoxia Inducible Factor (HIF) Alpha Subunit with in Vitro Nanomolar Affinities. (2014) J Med Chem. 57, 8657-8663;
doi. 10.1021/jm5011258;
PMID; 4207132, PMC25166285

Galvin, B. D., Li, Z., Villemaine, E., Poole, C. B., Chapman, M. S., Pollastri, M. P., Wyatt, P. G. and Carlow, C. K. A target repurposing approach identifies N-myristoyltransferase as a new candidate drug target in filarial nematodes. (2014) PLoS Negl Trop Dis. 8, e3145;
doi. 10.1371/journal.pntd.0003145;
PMID; 4154664, PMC25188325

Gilbert, I. H. Target-based drug discovery for human African trypanosomiasis: selection of molecular target and chemical matter. (2014) Parasitology. 141, 28-36;
doi. 10.1017/S0031182013001017;
PMID; 3884840, PMC23931634

Giordani, F., Buschini, A., Baliani, A., Kaiser, M., Brun, R., Barrett, M. P., Pellacani, C., Poli, P. and Gilbert, I. H. Characterization of a melamino nitroheterocycle as a potential lead for the treatment of human african trypanosomiasis. (2014) Antimicrob Agents Chemother. 58, 5747-5757;
doi. 10.1128/AAC.01449-13;
PMID; 4187918, PMC25022590

Glover, L. and Horn, D. Locus-specific control of DNA resection and suppression of subtelomeric VSG recombination by HAT3 in the African trypanosome. (2014) Nucleic Acids Res;
doi. 10.1093/nar/gku900;

Hobley, L., Kim, S. H., Maezato, Y., Wyllie, S., Fairlamb, A. H., Stanley-Wall, N. R. and Michael, A. J. Norspermidine is not a self-produced trigger for biofilm disassembly. (2014) Cell. 156, 844-854;
doi. 10.1016/j.cell.2014.01.012;
PMID; 3969229, PMC24529384

Holden, J. M., Koreny, L., Kelly, S., Chait, B. T., Rout, M. P., Field, M. C. and Obado, S. O. Touching from a distance: Evolution of interplay between the nuclear pore complex, nuclear basket, and the mitotic spindle. (2014) Nucleus. 5;
doi. PMID; 4152344, PMC25079062

Hopkins, A., Buchanan, G. and Palmer, T. Role of the twin arginine protein transport pathway in the assembly of the Streptomyces coelicolor cytochrome bc1 complex. (2014) J Bacteriol. 196, 50-59;
doi. 10.1128/JB.00776-13;

Hopkins, A. L., Keseru, G. M., Leeson, P. D., Rees, D. C. and Reynolds, C. H. The role of ligand efficiency metrics in drug discovery. (2014) Nat Rev Drug Discov. 13, 105-121;
doi. 10.1038/nrd4163;

Horn, D. High-throughput decoding of drug targets and drug resistance mechanisms in African trypanosomes. (2014) Parasitology. 141, 77-82;
doi. 10.1017/S0031182013000243;

Horn, D. Antigenic variation in African trypanosomes. (2014) Mol Biochem Parasitol. 195, 123-129;
doi. 10.1016/j.molbiopara.2014.05.001;
PMID; 4155160, PMC24859277

Horn, D. and Duraisingh, M. T. Antiparasitic chemotherapy: from genomes to mechanisms. (2014) Annu Rev Pharmacol Toxicol. 54, 71-94;
doi. 10.1146/annurev-pharmtox-011613-135915;

Khalaf, A. I., Huggan, J. K., Suckling, C. J., Gibson, C. L., Stewart, K., Giordani, F., Barrett, M. P., Wong, P. E., Barrack, K. L. and Hunter, W. N. Structure-based design and synthesis of antiparasitic pyrrolopyrimidines targeting pteridine reductase 1. (2014) J Med Chem. 57, 6479-6494;
doi. 10.1021/jm500483b;
PMID; 4136963, PMC25007262

Kneuper, H., Cao, Z. P., Twomey, K. B., Zoltner, M., Jager, F., Cargill, J. S., Chalmers, J., van der Kooi-Pol, M. M., van Dijl, J. M., Ryan, R. P., Hunter, W. N. and Palmer, T. Heterogeneity in ess transcriptional organization and variable contribution of the Ess/Type VII protein secretion system to virulence across closely related Staphylocccus aureus strains. (2014) Mol Microbiol. 93, 928-943;
doi. 10.1111/mmi.12707;
PMID; 4285178, PMC25040609

Lynch, M., Field, M. C., Goodson, H. V., Malik, H. S., Pereira-Leal, J. B., Roos, D. S., Turkewitz, A. P. and Sazer, S. Evolutionary cell biology: Two origins, one objective. (2014) Proc Natl Acad Sci U S A. 111, 16990-16994;
doi. 10.1073/pnas.1415861111;

MacGregor, P., Ivens, A., Shave, S., Collie, I., Gray, D., Auer, M. and Matthews, K. R. High-throughput chemical screening for antivirulence developmental phenotypes in Trypanosoma brucei. (2014) Eukaryot Cell. 13, 412-426;
doi. 10.1128/EC.00335-13;
PMID; 3957582, PMC24442893

Manna, P. T., Boehm, C., Leung, K. F., Natesan, S. K. and Field, M. C. Life and times: synthesis, trafficking, and evolution of VSG. (2014) Trends Parasitol. 30, 251-258;
doi. 10.1016/;
PMID; 4007029, PMC24731931

Miller, M. H., Walsh, S. V., Atrih, A., Huang, J. T., Ferguson, M. A. and Dillon, J. F. The serum proteome of nonalcoholic fatty liver disease: A multimodal approach to discovery of biomarkers of nonalcoholic steatohepatitis. (2014) J Gastroenterol Hepatol. 29, 1839-1847;
doi. 10.1111/jgh.12614;

Mony, B. M., MacGregor, P., Ivens, A., Rojas, F., Cowton, A., Young, J., Horn, D. and Matthews, K. Genome-wide dissection of the quorum sensing signalling pathway in Trypanosoma brucei. (2014) Nature. 505, 681-685;
doi. 10.1038/nature12864;
PMID; 3908871, PMC24336212

Munday, J. C., Eze, A. A., Baker, N., Glover, L., Clucas, C., Aguinaga Andres, D., Natto, M. J., Teka, I. A., McDonald, J., Lee, R. S., Graf, F. E., Ludin, P., Burchmore, R. J., Turner, C. M., Tait, A., MacLeod, A., Maser, P., Barrett, M. P., Horn, D. and De Koning, H. P. Trypanosoma brucei aquaglyceroporin 2 is a high-affinity transporter for pentamidine and melaminophenyl arsenic drugs and the main genetic determinant of resistance to these drugs. (2014) J Antimicrob Chemother. 69, 651-663;
doi. 10.1093/jac/dkt442;
PMID; 3922157, PMC24235095

Murray, C. W., Erlanson, D. A., Hopkins, A. L., Keseru, G. M., Leeson, P. D., Rees, D. C., Reynolds, C. H. and Richmond, N. J. Validity of ligand efficiency metrics. (2014) ACS Med Chem Lett. 5, 616-618;
doi. 10.1021/ml500146d;
PMID; 4060940, PMC24944729

Murungi, E., Barlow, L. D., Venkatesh, D., Adung'a, V. O., Dacks, J. B., Field, M. C. and Christoffels, A. A comparative analysis of trypanosomatid SNARE proteins. (2014) Parasitol Int. 63, 341-348;
doi. 10.1016/j.parint.2013.11.002;
PMID; 3979113, PMC24269876

Nakanishi, M., Karasudani, M., Shiraishi, T., Hashida, K., Hino, M., Ferguson, M. A. and Nomoto, H. TbGT8 is a bifunctional glycosyltransferase that elaborates N-linked glycans on a protein phosphatase AcP115 and a GPI-anchor modifying glycan in Trypanosoma brucei. (2014) Parasitol Int. 63, 513-518;
doi. 10.1016/j.parint.2014.01.007;
PMID; 4003530, PMC24508870

Neofytos, D., Huprikar, S., Reboli, A., Schuster, M., Azie, N., Franks, B. and Horn, D. Treatment and outcomes of Candida osteomyelitis: review of 53 cases from the PATH Alliance(R) registry. (2014) Eur J Clin Microbiol Infect Dis. 33, 135-141;
doi. 10.1007/s10096-013-1939-0;

Olivera-Martinez, I., Schurch, N., Li, R. A., Song, J., Halley, P. A., Das, R. M., Burt, D. W., Barton, G. J. and Storey, K. G. Major transcriptome re-organisation and abrupt changes in signalling, cell cycle and chromatin regulation at neural differentiation in vivo. (2014) Development. 141, 3266-3276;
doi. 10.1242/dev.112623;
PMID; 4197544, PMC25063452

Ong, H. B., Lee, W. S., Patterson, S., Wyllie, S. and Fairlamb, A. H. Homoserine and quorum-sensing acyl homoserine lactones as alternative sources of threonine: a potential role for homoserine kinase in insect-stage Trypanosoma brucei. (2014) Mol Microbiol;
doi. 10.1111/mmi.12853;

O'Rourke, P. E., Kalinowska-Tluscik, J., Fyfe, P. K., Dawson, A. and Hunter, W. N. Crystal structures of IspF from Plasmodium falciparum and Burkholderia cenocepacia: comparisons inform antimicrobial drug target assessment. (2014) BMC Struct Biol. 14, 1;
doi. 10.1186/1472-6807-14-1;
PMID; 3927217, PMC24410837

Pawellek, A., McElroy, S., Samatov, T., Mitchell, L., Woodland, A., Ryder, U., Gray, D., Luhrmann, R. and Lamond, A. I. Identification of small molecule inhibitors of pre-mRNA splicing. (2014) J Biol Chem. 289, 34683-34698;
doi. 10.1074/jbc.M114.590976;
PMID; 4263873, PMC25281741

Peng, X., Alfoldi, J., Gori, K., Eisfeld, A. J., Tyler, S. R., Tisoncik-Go, J., Brawand, D., Law, G. L., Skunca, N., Hatta, M., Gasper, D. J., Kelly, S. M., Chang, J., Thomas, M. J., Johnson, J., Berlin, A. M., Lara, M., Russell, P., Swofford, R., Turner-Maier, J., Young, S., Hourlier, T., Aken, B., Searle, S., Sun, X., Yi, Y., Suresh, M., Tumpey, T. M., Siepel, A., Wisely, S. M., Dessimoz, C., Kawaoka, Y., Birren, B. W., Lindblad-Toh, K., Di Palma, F., Engelhardt, J. F., Palermo, R. E. and Katze, M. G. The draft genome sequence of the ferret (Mustela putorius furo) facilitates study of human respiratory disease. (2014) Nat Biotechnol. 32, 1250-1255;
doi. 10.1038/nbt.3079;
PMID; 4262547, PMC25402615

Perez, E., Lapaille, M., Degand, H., Cilibrasi, L., Villavicencio-Queijeiro, A., Morsomme, P., Gonzalez-Halphen, D., Field, M. C., Remacle, C., Baurain, D. and Cardol, P. The mitochondrial respiratory chain of the secondary green alga Euglena gracilis shares many additional subunits with parasitic Trypanosomatidae. (2014) Mitochondrion;
doi. 10.1016/j.mito.2014.02.001;

Porcel, B. M., Denoeud, F., Opperdoes, F., Noel, B., Madoui, M. A., Hammarton, T. C., Field, M. C., Da Silva, C., Couloux, A., Poulain, J., Katinka, M., Jabbari, K., Aury, J. M., Campbell, D. A., Cintron, R., Dickens, N. J., Docampo, R., Sturm, N. R., Koumandou, V. L., Fabre, S., Flegontov, P., Lukes, J., Michaeli, S., Mottram, J. C., Szoor, B., Zilberstein, D., Bringaud, F., Wincker, P. and Dollet, M. The streamlined genome of Phytomonas spp. relative to human pathogenic kinetoplastids reveals a parasite tailored for plants. (2014) PLoS Genet. 10, e1004007;
doi. 10.1371/journal.pgen.1004007;
PMID; 3916237, PMC24516393

Rimsa, V., Eadsforth, T. C., Joosten, R. P. and Hunter, W. N. High-resolution structure of the M14-type cytosolic carboxypeptidase from Burkholderia cenocepacia refined exploiting PDB_REDO strategies. (2014) Acta Crystallogr D Biol Crystallogr. 70, 279-289;
doi. 10.1107/S1399004713026801;
PMID; 3940198, PMC24531462

Roberts, A. J., Torrie, L. S., Wyllie, S. and Fairlamb, A. H. Biochemical and genetic characterization of Trypanosoma cruzi N-myristoyltransferase. (2014) Biochem J. 459, 323-332;
doi. 10.1042/BJ20131033;
PMID; 3969225, PMC24444291

Schlacht, A., Herman, E. K., Klute, M. J., Field, M. C. and Dacks, J. B. Missing Pieces of an Ancient Puzzle: Evolution of the Eukaryotic Membrane-Trafficking System. (2014) Cold Spring Harb Perspect Biol. 6;
doi. 10.1101/cshperspect.a016048;

Schurch, N. J., Cole, C., Sherstnev, A., Song, J., Duc, C., Storey, K. G., McLean, W. H., Brown, S. J., Simpson, G. G. and Barton, G. J. Improved Annotation of 3' Untranslated Regions and Complex Loci by Combination of Strand-Specific Direct RNA Sequencing, RNA-Seq and ESTs. (2014) PLoS One. 9, e94270;
doi. 10.1371/journal.pone.0094270;

Speakman, C. M., Domke, T. C., Wongpaiboonwattana, W., Sanders, K., Mudaliar, M., van Aalten, D. M., Barton, G. J. and Stavridis, M. P. Elevated O-GlcNAc levels activate epigenetically repressed genes and delay mouse ESC differentiation without affecting naive to primed cell transition. (2014) Stem Cells. 32, 2605-2615;
doi. 10.1002/stem.1761;

Sternberg, J. M., Gierlinski, M., Bieler, S., Ferguson, M. A. and Ndung'u, J. M. Evaluation of the diagnostic accuracy of prototype rapid tests for human African trypanosomiasis. (2014) PLoS Negl Trop Dis. 8, e3373;
doi. 10.1371/journal.pntd.0003373;
PMID; 4270746, PMC25521120

Steverding, D., Michaels, S. and Read, K. D. In vitro and in vivo studies of trypanocidal activity of dietary isothiocyanates. (2014) Planta Med. 80, 183-186;
doi. 10.1055/s-0033-1360262;

Sullivan, L., Fleming, J., Sastry, L., Mehlert, A., Wall, S. J. and Ferguson, M. A. Identification of sVSG117 as an immunodiagnostic antigen and evaluation of a dual-antigen lateral flow test for the diagnosis of human African trypanosomiasis. (2014) PLoS Negl Trop Dis. 8, e2976;
doi. 10.1371/journal.pntd.0002976;
PMID; 4102454, PMC25033401

Urbaniak, M. D., Capes, A. S., Crossman, A., O'Neill, S., Thompson, S., Gilbert, I. H. and Ferguson, M. A. Fragment screening reveals salicylic hydroxamic acid as an inhibitor of Trypanosoma brucei GPI GlcNAc-PI de-N-acetylase. (2014) Carbohydr Res. 387, 54-58;
doi. 10.1016/j.carres.2013.12.016;
PMID; 3991331, PMC24589444

Urich, R., Grimaldi, R., Luksch, T., Frearson, J. A., Brenk, R. and Wyatt, P. G. The Design and Synthesis of Potent and Selective Inhibitors of Trypanosoma brucei Glycogen Synthase Kinase 3 for the Treatment of Human African Trypanosomiasis. (2014) J Med Chem. 57, 7536-7549;
doi. 10.1021/jm500239b;
PMID; 4175002, PMC25198388

Wideman, J. G., Leung, K. F., Field, M. C. and Dacks, J. B. The cell biology of the endocytic system from an evolutionary perspective. (2014) Cold Spring Harb Perspect Biol. 6, a016998;
doi. 10.1101/cshperspect.a016998;