University of Dundee

MRC Protein Phosphorylation Unit 2009

Akue-Gedu, R., Rossignol, E., Azzaro, S., Knapp, S., Filippakopoulos, P., Bullock, A. N., Bain, J., Cohen, P., Prudhomme, M., Anizon, F. and Moreau, P. (2009) Synthesis, kinase inhibitory potencies, and in vitro antiproliferative evaluation of new Pim kinase inhibitors. J Med Chem. 52, 6369-6381

Alessi, D. R., Pearce, L. R. and Garcia-Martinez, J. M. (2009) New insights into mTOR signaling: mTORC2 and beyond. Sci Signal. 2, pe27

Arthur, J. S. and McGuire, V. A. (2009) Gene-targeting vectors. Methods Mol Biol. 561, 127-144

Babraj, J. A., Mustard, K., Sutherland, C., Towler, M. C., Chen, S., Smith, K., Green, K., Leese, G., Hardie, D. G., Rennie, M. J. and Cuthbertson, D. J. (2009) Blunting of AICAR-induced human skeletal muscle glucose uptake in type 2 diabetes is dependent on age rather than diabetic status. Am J Physiol Endocrinol Metab. 296, E1042-1048

Boini, K. M., Amann, K., Kempe, D., Alessi, D. R. and Lang, F. (2009) Proteinuria in mice expressing PKB/SGK-resistant GSK3. Am J Physiol Renal Physiol. 296, F153-159

Brady, S. C., Coleman, M. L., Munro, J., Feller, S. M., Morrice, N. A. and Olson, M. F. (2009) Sprouty2 association with B-Raf is regulated by phosphorylation and kinase conformation. Cancer Res. 69, 6773-6781

Chen, S. and Mackintosh, C. (2009) Differential regulation of NHE1 phosphorylation and glucose uptake by inhibitors of the ERK pathway and p90RSK in 3T3-L1 adipocytes. Cell Signal. 21, 1984-1993

Clark, K., Plater, L., Peggie, M. and Cohen, P. (2009) Use of the Pharmacological Inhibitor BX795 to Study the Regulation and Physiological Roles of TBK1 and I{kappa}B Kinase {epsilon}: A DISTINCT UPSTREAM KINASE MEDIATES SER-172 PHOSPHORYLATION AND ACTIVATION. J Biol Chem. 284, 14136-14146

Cohen, P. (2009) Keep nibbling at the edges. J Biol Chem. 284, 23891-23901

Cohen, P. (2009) Targeting protein kinases for the development of anti-inflammatory drugs. Curr Opin Cell Biol. 21, 317-324

Cohen, P. (2009) Profile of Philip Cohen. Interview by Carrie Arnold. Proc Natl Acad Sci U S A. 106, 7692-7694

Cohen, P. (2009) Dundee: from Jute, Jam and Journalism to Biochemistry, Biomedicine and Biotechnology. The New Scottish Enlightenment. Times Group, 122-129

Cohen, P. T. W. (2009) Phosphatase families dephosphorylating serine and threonine residues in proteins. In Handbook of Cell Signaling (Bradshaw, R. A. D., E. A., ed.). pp. 659-675, Elsevier Inc. Academic Press, Oxford

Dettori, R., Sonzogni, S., Meyer, L., Lopez-Garcia, L. A., Morrice, N. A., Zeuzem, S., Engel, M., Piiper, A., Neimanis, S., Frodin, M. and Biondi, R. M. (2009) Regulation of the interaction between protein kinase C-related protein kinase 2 (PRK2) and its upstream kinase, 3-phosphoinositide-dependent protein kinase 1 (PDK1). J Biol Chem. 284, 30318-30327

Dubois, F., Vandermoere, F., Gernez, A., Murphy, J., Toth, R., Chen, S., Geraghty, K. M., Morrice, N. A. and Mackintosh, C. (2009) Differential 14-3-3 affinity capture reveals new downstream targets of phosphatidylinositol 3-kinase signaling. Mol Cell Proteomics. 8, 2487-2499

Fuller, W., Howie, J., McLatchie, L. M., Weber, R. J., Hastie, C. J., Burness, K., Pavlovic, D. and Shattock, M. J. (2009) FXYD1 phosphorylation in vitro and in adult rat cardiac myocytes: threonine 69 is a novel substrate for protein kinase C. Am J Physiol Cell Physiol. 296, C1346-1355

Garcia-Martinez, J. M., Moran, J., Clarke, R. G., Gray, A., Cosulich, S. C., Chresta, C. M. and Alessi, D. R. (2009) Ku-0063794 is a specific inhibitor of the mammalian target of rapamycin (mTOR). Biochem J. 421, 29-42

Guigas, B., Sakamoto, K., Taleux, N., Reyna, S. M., Musi, N., Viollet, B. and Hue, L. (2009) Beyond AICA riboside: in search of new specific AMP-activated protein kinase activators. IUBMB Life. 61, 18-26

Henderson, G., Craig, S., Baier, R. J., Helps, N., Brocklehurst, P. and McGuire, W. (2009) Cytokine gene polymorphisms in preterm infants with necrotising enterocolitis: genetic association study. Arch Dis Child Fetal Neonatal Ed. 94, F124-128

Hunter, M. J., McLaren-Lee, I. and Helps, N. R. (2009) Extract-N-Amp Reagen Provides a Highly Effective Way of Generating PCR Products from FTA Cards. Life Science Innovations (Sigma Life Science). 23, 3-4

Hunter, R. W., Mackintosh, C. and Hers, I. (2009) Protein Kinase C-mediated Phosphorylation and Activation of PDE3A Regulate cAMP Levels in Human Platelets. J Biol Chem. 284, 12339-12348

Inesta-Vaquera, F. A., Centeno, F., del Reino, P., Sabio, G., Peggie, M. and Cuenda, A. (2009) Proteolysis of the tumour suppressor hDlg in response to osmotic stress is mediated by caspases and independent of phosphorylation. FEBS J. 276, 387-400

Inglis, S. K., Gallacher, M., Brown, S. G., McTavish, N., Getty, J., Husband, E. M., Murray, J. T. and Wilson, S. M. (2009) SGK1 activity in Na(+) absorbing airway epithelial cells monitored by assaying NDRG1-Thr(346/356/366) phosphorylation. Pflugers Arch. 457, 1287-1301

Kelsall, I. R., Rosenzweig, D. and Cohen, P. T. W. (2009) Disruption of the allosteric phosphorylase a regulation of the hepatic glycogen-targeted protein phosphatase 1 improves glucose tolerance in vivo. Cell Signal. 21, 1123-1134

Klevernic, I. V., Martin, N. M. and Cohen, P. (2009) Regulation of the activity and expression of ERK8 by DNA damage. FEBS Lett. 583, 680-684

Klevernic, I. V., Morton, S., Davis, R. J. and Cohen, P. (2009) Phosphorylation of Ewing's sarcoma protein (EWS) and EWS-Fli1 in response to DNA damage. Biochem J. 418, 625-634

Kucharska, A., Rushworth, L. K., Staples, C., Morrice, N. A. and Keyse, S. M. (2009) Regulation of the inducible nuclear dual-specificity phosphatase DUSP5 by ERK MAPK. Cell Signal. 21, 1794-1805

Libby, G., Donnelly, L. A., Donnan, P. T., Alessi, D. R., Morris, A. D. and Evans, J. M. (2009) New users of metformin are at low risk of incident cancer: a cohort study among people with type 2 diabetes. Diabetes Care. 32, 1620-1625

MacKay, C., Toth, R. and Rouse, J. (2009) Biochemical characterisation of the SWI/SNF family member HLTF. Biochem Biophys Res Commun. 390, 187-191

Mikkelsen, S. S., Jensen, S. B., Chiliveru, S., Melchjorsen, J., Julkunen, I., Gaestel, M., Arthur, J. S., Flavell, R. A., Ghosh, S. and Paludan, S. R. (2009) RIG-I-mediated Activation of p38 MAPK Is Essential for Viral Induction of Interferon and Activation of Dendritic Cells: DEPENDENCE ON TRAF2 AND TAK1. J Biol Chem. 284, 10774-10782

Mounier, R., Lantier, L., Leclerc, J., Sotiropoulos, A., Pende, M., Daegelen, D., Sakamoto, K., Foretz, M. and Viollet, B. (2009) Important role for AMPKalpha1 in limiting skeletal muscle cell hypertrophy. FASEB J. 23, 2264-2273

Munoz, I. M., Hain, K., Declais, A. C., Gardiner, M., Toh, G. W., Sanchez-Pulido, L., Heuckmann, J. M., Toth, R., Macartney, T., Eppink, B., Kanaar, R., Ponting, C. P., Lilley, D. M. and Rouse, J. (2009) Coordination of structure-specific nucleases by human SLX4/BTBD12 is required for DNA repair. Mol Cell. 35, 116-127

Munoz, I. M. and Rouse, J. (2009) Control of histone methylation and genome stability by PTIP. EMBO Rep. 10, 239-245

Muqit, M. M. and Alessi, D. R. (2009) A meeting of minds: overcoming roadblocks in the development of therapies for neurodegenerative disorders. EMBO Mol Medicine. 1, 139-141 (Read online)

Nichols, R. J., Dzamko, N., Hutti, J. E., Cantley, L. C., Deak, M., Moran, J., Bamborough, P., Reith, A. D. and Alessi, D. R. (2009) Substrate specificity and inhibitors of LRRK2, a protein kinase mutated in Parkinson's disease. Biochem J. 424, 47-60

Pehmoller, C., Treebak, J. T., Birk, J. B., Chen, S., Mackintosh, C., Hardie, D. G., Richter, E. A. and Wojtaszewski, J. F. (2009) Genetic disruption of AMPK signaling abolishes both contraction- and insulin-stimulated TBC1D1 phosphorylation and 14-3-3 binding in mouse skeletal muscle. Am J Physiol Endocrinol Metab. 297, E665-675

Peifer, C. and Alessi, D. R. (2009) New anti-cancer role for PDK1 inhibitors: preventing resistance to tamoxifen. Biochem J. 417, e5-7

Rouse, J. (2009) Control of genome stability by SLX protein complexes. Biochem Soc Trans. 37, 495-510

San-Cristobal, P., Pacheco-Alvarez, D., Richardson, C., Ring, A. M., Vazquez, N., Rafiqi, F. H., Chari, D., Kahle, K. T., Leng, Q., Bobadilla, N. A., Hebert, S. C., Alessi, D. R., Lifton, R. P. and Gamba, G. (2009) Angiotensin II signaling increases activity of the renal Na-Cl cotransporter through a WNK4-SPAK-dependent pathway. Proc Natl Acad Sci U S A. 106, 4384-4389

Schindler, E. M., Hindes, A., Gribben, E. L., Burns, C. J., Yin, Y., Lin, M. H., Owen, R. J., Longmore, G. D., Kissling, G. E., Arthur, J. S. and Efimova, T. (2009) p38delta Mitogen-activated protein kinase is essential for skin tumor development in mice. Cancer Res. 69, 4648-4655

Sleiman, P. M., Healy, D. G., Muqit, M. M., Yang, Y. X., Van Der Brug, M., Holton, J. L., Revesz, T., Quinn, N. P., Bhatia, K., Diss, J. K., Lees, A. J., Cookson, M. R., Latchman, D. S. and Wood, N. W. (2009) Characterisation of a novel NR4A2 mutation in Parkinson's disease brain. Neurosci Lett. 457, 75-79

Smith, H., Peggie, M., Campbell, D. G., Vandermoere, F., Carrick, E. and Cohen, P. (2009) Identification of the phosphorylation sites on the E3 ubiquitin ligase Pellino that are critical for activation by IRAK1 and IRAK4. Proc Natl Acad Sci U S A. 106, 4584-4590

Treebak, J. T., Frosig, C., Pehmoller, C., Chen, S., Maarbjerg, S. J., Brandt, N., MacKintosh, C., Zierath, J. R., Hardie, D. G., Kiens, B., Richter, E. A., Pilegaard, H. and Wojtaszewski, J. F. (2009) Potential role of TBC1D4 in enhanced post-exercise insulin action in human skeletal muscle. Diabetologia. 52, 891-900

von Kriegsheim, A., Baiocchi, D., Birtwistle, M., Sumpton, D., Bienvenut, W., Morrice, N., Yamada, K., Lamond, A., Kalna, G., Orton, R., Gilbert, D. and Kolch, W. (2009) Cell fate decisions are specified by the dynamic ERK interactome. Nat Cell Biol. 11, 1458-1464

Wingate, A. D., Martin, K. J., Hunter, C., Carr, J. M., Clacher, C. and Arthur, J. S. (2009) Generation of a conditional CREB Ser133Ala knockin mouse. Genesis. 47, 688-696

Wood, N. T., Meek, D. W. and Mackintosh, C. (2009) 14-3-3 Binding to Pim-phosphorylated Ser166 and Ser186 of human Mdm2--Potential interplay with the PKB/Akt pathway and p14(ARF). FEBS Lett. 583, 615-620

Yang, H. T., Peggie, M., Cohen, P. and Rousseau, S. (2009) DAZAP1 interacts via its RNA-recognition motifs with the C-termini of other RNA-binding proteins. Biochem Biophys Res Commun. 380, 705-709

Zeqiraj, E., Filippi, B. M., Deak, M., Alessi, D. R. and van Aalten, D. M. (2009) Structure of the LKB1-STRAD-MO25 complex reveals an allosteric mechanism of kinase activation. Science. 326, 1707-1711

Zeqiraj, E., Filippi, B. M., Goldie, S., Navratilova, I., Boudeau, J., Deak, M., Alessi, D. R. and van Aalten, D. M. (2009) ATP and MO25alpha regulate the conformational state of the STRADalpha pseudokinase and activation of the LKB1 tumour suppressor. PLoS Biol. 7, e1000126