1. Bustos, F. et al. Activity-based probe profiling of RNF12 E3 ubiquitin ligase function in Tonne-Kalscheuer syndrome. Life Sci Alliance, 5, e202101248 (2022).

2. Spear, L. A. et al. Selective Inhibition of Cysteine-Dependent Enzymes by Bioorthogonal Tethering. J Mol Biol 434, 167524 (2022).

3. Cesare, V. D. et al. Deubiquitinating enzyme amino acid profiling reveals a class of ubiquitin esterases. Proc National Acad Sci 118, e2006947118 (2021).

       Highlighted in:  Kessler, B. M. DUB esterase activity further decodes ubiquitin's enigma. Proc National Acad Sci 118, e2026389118 (2021).  

4. Mabbitt, P. D. et al. Structural basis for RING-Cys-Relay E3 ligase activity and its role in axon integrity. Nat Chem Biol 16, 1227-1236 (2020).

       Highlighted in:  Faculty Opinions

                                 European Synchrotron Radiation Facility (ESRF) Annual Highlights 2020 

                                 Lorenz, S. & Rittinger, K. Advanced rules of relays. Nat Chem Biol 16, 1158-1159 (2020).  


5. Mathur, S., Fletcher, A. J., Branigan, E., Hay, R. T. & Virdee, S. Photocrosslinking Activity-Based Probes for Ubiquitin RING E3 Ligases. Cell Chem Biol 27, 74-82.e6 (2019).

6. Pao, K.-C. et al. Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity. Nature 556, 381-385 (2018).

       Highlighted in: Faculty Opinions 

                                 European Synchrotron Radiation Facility (ESRF) Spotlight on Science

                                 European Synchrotron Radiation Facility (ESRF) Annual Highlights 2018 

7. Kwasna, D. et al. Discovery and Characterization of ZUFSP/ZUP1, a Distinct Deubiquitinase Class Important for Genome Stability. Mol Cell 70, 150-164.e6 (2018).

8. Pao, K.-C., Stanley, M., Han, C., Lai, Y.-C., Murphy, P., Balk, P., Wood, N., Corti, O., Corvol, J.-C., Muqit, M. M. K., & Virdee, S. Probes of ubiquitin E3 ligases enable systematic dissection of parkin activation. (2016) Nat Chem Biol 12, 324-331.

9. Han, C., Pao, K-C., Kazlauskaite, A., Muqit, M. M. K. & Virdee, S. A Versatile Strategy for the Semisynthetic Production of Ser65 Phosphorylated Ubiquitin and its Biochemical and Structural Characterization. (2015) Chembiochem, 16, 1574-1579.

10. Stanley, M., Han, C., Knebel, A., Murphy, P., Shpiro, N. & Virdee, S. Orthogonal Thiol Functionalization at a Single Atomic Center for Profiling Transthiolation Activity of E1 Activating Enzymes. (2015) ACS Chem Biol 10, 1542-1554.

11. Bett, J. S., Ritorto, M. S., Ewan, R., Jaffray, E. G., Virdee, S., Chin, J. W., Knebel, A., Kurz, T., Trost, M., Tatham, M. H. & Hay, R. T. (2015) Ubiquitin C-terminal hydrolases cleave isopeptide- and peptide- linked ubiquitin from structured proteins but do not edit ubiquitin homopolymers. Biochem. J. 466, 489-498.

       Highlighted in: Faculty Opinions


12. Ritorto, M. S., Ewan, R., Perez-Oliva, A. B., Knebel, A., Buhrlage, S. J., Wightman, M., Kelly, S. M., Wood, N. T., Virdee, S., Gray, N. S., Morrice, N. A., Alessi, D. R., & Trost, M. Screening of DUB activity and specificity by MALDI-TOF mass spectrometry. (2014) Nat. Commun. 5, 4763.

13. Licchesi, J. D. F., Mieszczanek, J., Mevissen, T. E. T., Rutherford, T. R., Akutsu, M., Virdee, S., Oualid, F., E., Chin, J. W., Ovaa, H., Bienz, M. & Komander, D. An ankyrin-repeat ubiquitin-binding domain determines TRABID's specificity for atypical ubiquitin chains. (2012) Nat. Struct. Mol. Biol. 19, 62-71.

14. Virdee, S., Kapadnis, P., Elliot, T., Lang, K., Madrzak, J., Nguyen, D.P., Riechmann, L. & Chin, J.W. (2011) Traceless and site-specific ubiquitination of recombinant proteins. J. Am. Chem. Soc. 133, 10708-10711.


       Highlighted in: Faculty Opinions 

                                 Chemical and Engineering News 2010

                                Chemical and Engineering Chemical Year in Review 2010

                                  Angewandte Chemie.  


15. Akutsu, M., Ye, Y., Virdee, S., Chin, J. W. & Komander, D. Molecular basis for ubiquitin and ISG15 cross-reactivity in viral ovarian tumor domains. (2011) Proc. Nat.l Acad. Sci. U S A 108, 2228-2233.

16. Nguyen, D. P., Alai, M. M. G., Virdee, S. & Chin, J. W. Genetically Directing ɛ-N, N-Dimethyl-L- Lysine in Recombinant Histones. (2010) Chem. Biol. 17, 1072-1076.

       Highlighted in: Faculty Opinions


17. Virdee, S., Ye, Y., Nguyen, D. P., Komander, D. & Chin, J. W. Engineered diubiquitin synthesis reveals Lys29-isopeptide specificity of an OTU deubiquitinase. (2010) Nat. Chem. Biol. 6, 750-757.

18. Virdee, S., Macmillan, D. & Waksman, G. Semisynthetic Src SH2 domains demonstrate altered phosphopeptide specificity induced by incorporation of unnatural lysine derivatives. (2010) Chem. Biol. 17, 274-284.

19. Geroult, S., Hooda, M., Virdee, S. & Waksman, G. Prediction of solvation sites at the interface of Src SH2 domain complexes using molecular dynamics simulations. (2007) Chem. Biol. Drug Des. 70, 87-99.

20. Geroult, S., Virdee, S. & Waksman, G. The role of water in computational and experimental derivation of binding thermodynamics in SH2 domains. (2006) Chem. Biol. Drug Des. 67, 38-45.



Squair, D.S. & Virdee, S. A new dawn beyond lysine ubiquitination. (2022)  Nat. Chem. Biol. 18, 802-811.

Virdee, S. An atypical ubiquitin ligase at the heart of neural development and programmed axon degeneration. (2022) Neural Regen Res 17, 2347-2350.

Virdee, S.  2016 EMBO Chemical  Biology Conference. (2017) Chembiochem, 18, 66-71.

Stanley, M. & Virdee, S. Chemical ubiquitination for decrypting a cellular code. (2016) Biochem. J. 473, 1297-1314.

Komatsu, T. & Virdee, S. ICBS and ECBS Chemical Biology Meeting 2015 - Let Them Come to Berlin! (2016) ACS Chem. Biol. 11, 1159-1166.


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