Email: E.G.Jaffray@dundee.ac.uk

I obtained my HNC in Biological Sciences from the University of Abertay in Dundee and took up a position in the Department of Biochemistry at the University of St Andrews. In 1992 I started working with Ron Hay in the then Department of Biochemistry and Microbiology at the University of St Andrews. In 1998 we moved within St Andrews to the new Centre for Biomolecular Sciences and in 2005 we moved to the University of Dundee.

Refs

A SUMO-Dependent Protein Network Regulates Chromosome Congression during Oocyte Meiosis. Pelisch F, Tammsalu T, Wang B, Jaffray EG, Gartner A, Hay RT. Mol Cell. 2017 Jan 5;65(1):66-77. doi: 10.1016/j.molcel.2016.11.001. Epub 2016 Dec 8. PMID: 27939944

Loss of ubiquitin E2 Ube2w rescues hypersensitivity of Rnf4 mutant cells to DNA damage. Maure JF, Moser SC, Jaffray EG, F Alpi A, Hay RT. Sci Rep. 2016 May 17;6:26178. doi: 10.1038/srep26178.
PMID: 27185577

Proteome-wide identification of SUMO modification sites by mass spectrometry. Tammsalu T, Matic I, Jaffray EG, Ibrahim AF, Tatham MH, Hay RT. Nat Protoc. 2015 Sep;10(9):1374-88. doi: 10.1038/nprot.2015.095. Epub 2015 Aug 20. PMID: 26292070

Structural basis for the RING-catalyzed synthesis of K63-linked ubiquitin chains. Branigan E, Plechanovová A, Jaffray EG, Naismith JH, Hay RT. Nat Struct Mol Biol. 2015 Aug;22(8):597-602. doi: 10.1038/nsmb.3052. Epub 2015 Jul 6. PMID: 26148049

Ubiquitin-C-terminal hydrolases cleave isopeptide and peptide-linked ubiquitin from structured proteins but do not edit ubiquitin homopolymers. Bett JS, Ritorto MS, Ewan R, Jaffray EG, Virdee S, Chin JW, Knebel A, Kurz T, Trost M, Tatham MH, Hay RT. Biochem J. 2014 Dec 9. [Epub ahead of print] PMID: 25489924

Proteome-wide identification of SUMO2 modification sites. Tammsalu T, Matic I, Jaffray EG, Ibrahim AF, Tatham MH, Hay RT. Sci Signal. 2014 Apr 29;7(323):rs2. doi: 10.1126/scisignal.2005146. PMID: 24782567

SUMO chain-induced dimerization activates RNF4. Rojas-Fernandez A, Plechanovová A, Hattersley N, Jaffray E, Tatham MH, Hay RT. Mol Cell. 2014 Mar 20;53(6):880-92. doi: 10.1016/j.molcel.2014.02.031. PMID: 24656128

Pozzebon ME, Varadaraj A, Mattoscio D, Jaffray EG, Miccolo C, Galimberti V, Tommasino M, Hay RT, Chiocca S.BC-box protein domain-related mechanism for VHL protein degradation. Proc Natl Acad Sci U S A. 2013 Nov 5;110(45):18168-73. doi: 10.1073/pnas.1311382110. Epub 2013 Oct 21. .

Citro S, Jaffray E, Hay RT, Seiser C, Chiocca S. A role for paralog-specific sumoylation in histone deacetylase 1 stability. J Mol Cell Biol. 2013 Dec;5(6):416-27. doi: 10.1093/jmcb/mjt032. Epub 2013 Sep 25. .

Tatham MH, Plechanovová A, Jaffray EG, Salmen H, Hay RT. Ube2W conjugates ubiquitin to α-amino groups of protein N-termini. Biochem J. 2013 Jul 1;453(1):137-45. doi: 10.1042/BJ20130244.

Plechanovovà A, Jaffray E, Tatham MH, Naismith JH, Hay RT. (2012) Structure of a RING E3 ligase and ubiquitin-loaded E2 primed for catalysis. Nature 489, 115-120

Matic I, Jaffray EG, Oxenham SK, Groves MJ, Barratt CL, Tauro S, Stanley-Wall NR, Hay RT. Absolute SILAC-compatible expression strain allows Sumo-2 copy number determination in clinical samples. J Proteome Res. 2011 Oct 7;10(10):4869-75. Epub 2011 Aug 25.

Plechanovová A, Jaffray EG, McMahon SA, Johnson KA, Navrátilová I, Naismith JH, Hay RT. Mechanism of ubiquitylation by dimeric RING ligase RNF4. Nat Struct Mol Biol. 2011 Aug 21;18(9):1052-9. doi: 10.1038/nsmb.2108.

Castillo-Lluva S, Tatham MH, Jones RC, Jaffray EG, Edmondson RD, Hay RT, Malliri A. SUMOylation of the GTPase Rac1 is required for optimal cell migration. Nat Cell Biol. 2010 Nov;12(11):1078-85. Epub 2010 Oct 10.

Hattersley N, Shen L, Jaffray EG, Hay RT. The SUMO protease SENP6 is a direct regulator of PML nuclear bodies. Mol Biol Cell. 2011 Jan 1;22(1):78-90. Epub 2010 Dec 9.

Geoffroy MC, Jaffray EG, Walker KJ, Hay RT.Arsenic-induced SUMO-dependent recruitment of RNF4 into PML nuclear bodies. Mol Biol Cell. 2010 Dec;21(23):4227-39. Epub 2010 Oct 13.

Napolitano LM, Jaffray EG, Hay RT, Meroni G. Functional interactions between ubiquitin E2 enzymes and TRIM proteins. Biochem J. 2011 Mar 1;434(2):309-19.

Shen LN, Geoffroy MC, Jaffray EG, Hay RT. Characterization of SENP7, a SUMO-2/3-specific isopeptidase. Biochem J. 2009 Jun 26;421(2):223-30.PMID: 19392659

Miles WO, Jaffray E, Campbell SG, Takeda S, Bayston LJ, Basu SP, Li M, Raftery LA, Ashe MP, Hay RT, Ashe HL. Medea SUMOylation restricts the signaling range of the Dpp morphogen in the Drosophila embryo. Genes Dev. 2008 Sep 15;22(18):2578-90.

Tatham MH, Geoffroy MC, Shen L, Plechanovova A, Hattersley N, Jaffray EG, Palvimo JJ, Hay RT. RNF4 is a poly-SUMO-specific E3 ubiquitin ligase required for arsenic-induced PML degradation. Nat Cell Biol. 2008 May; 10(5):538-46.

Jaffray EG, Hay RT. Detection of modification by ubiquitin-like proteins. Methods. 2006 Jan; 38(1):35-8.

Tatham MH, Kim S, Jaffray E, Song J, Chen Y, Hay RT. Unique binding interactions among Ubc9, SUMO and RanBP2 reveal a mechanism for SUMO paralog selection. Nat Struct Mol Biol. 2005 Jan;12(1):67-74.

Yang SH, Jaffray E, Hay RT, Sharrocks AD. Dynamic interplay of the SUMO and ERK pathways in regulating Elk-1transcriptional activity. Mol Cell. 2003 Jul; 12(1):63-74.