Plasmids
Several of these constructs are also available from Addgene.
Click here to view inducible expression data.
For more information on cells and vectors see: Inducible cell lines (pdf), and George Cross and Christine Clayton’s web-sites.
Resources
Tagging
| Plasmid | Map (PDF) | Sequence | Description |
|---|---|---|---|
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pRPaTAG |
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Tet-inducible RRNA promoter driven plasmid for expression of N- or C-terminally tagged proteins from a specific RRNA spacer locus in T. brucei. Variants available (HYG): pRPaiGFPx, pRPaixGFP, pRPaiMYCx, pRPaix6MYCx Constitutive expression plasmids, pRPaGFPx and pRPax6MYCx, are also available |
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pRPTAG |
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Tet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally tagged proteins from a random RRNA spacer locus in T. brucei. Variants available (HYG): pRPiGFPx, pRPiMYCx (also BSD), pRPix6MYCx (also BSD) Constitutive expression plasmids (BSD), pRPxGFP and pRPx6MYCx, are also available |
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pT7TAG |
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Tet-inducible T7 promoter driven plasmid for inducible expression of N-terminally tagged proteins from a random RRNA spacer in T. brucei. Variants available (HYG): pT7iGFPx, pRPiMYCx |
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pNATTAGx |
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A plasmid for expression of N-terminally tagged proteins from a gene’s native locus. Variants available (BSD and HYG): pNATGFPx, pNAT6MYCx Details (pdf) in preparation |
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pNATxTAG |
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A plasmid for expression of C-terminally tagged proteins from a gene’s native locus. Variants available (BSD and HYG): pNATxGFP, pNATx12MYC Details (pdf) in preparation |
RNAi
| Plasmid | Map (PDF) | Sequence | Description |
|---|---|---|---|
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pRPaiSL |
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Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei. |
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p2T7TAblue |
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Head-to-head T7 promoter-driven plasmid for the generation of dsRNA for RNAi experiments in T. brucei. p2T7aTAblue (sequence) is also available – compatible with the tagged locus cell line when modified to express T7 polymerase. |