Protein Target(s) Name: E3 ubiquitin ligase VHL

Mechanism of Action: PROTAC degrader

Description: Homo-PROTAC dimerizer for self-degradation of the von Hippel-Lindau (VHL) protein. CM11 induces selective degradation of the long isoform of VHL (pVHL30) over the short isoform (pVHL19), and does so without triggering a hypoxic response in cells. CM11 also induces depletion of scaffold protein Cul2 in cells (1).

Chemical Name: N1,N20-bis((S)-1-((2S,4R)-4-hydroxy-2-((4-(4-methylthiazol-5-yl)benzyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)-3,6,9,12,15,18-hexaoxaicosanediamide

CAS Number: 2244684-49-7

In vitro pharmacology*: CM11 reduces pVHL30 protein levels in human cells: pVHL30 DC50 between 1-10 nM in HeLa cells (4 h); Dmax > 99%. CM11 induces time-dependent degradation of pVHL30, depleting 50% protein between 1-2 h of treatment at 1 uM in HeLa cells. CM11 (1 uM, 24 h) also induced 73% depletion of Cul2 in HeLa cells. Data from ref. (1)

*DC50: concentration in molar causing 50% reduction of protein level relative to vehicle control treatment.
Dmax: maximum reduction of protein level relative to vehicle control treatment.

Biophysical binding data: ITC Kd (pVHL30) = 25 nM; ITC Kd (pVHL19) = 11 nM; stoichiometry n = 0.6 (2:1 VHL:CM11 complex); cooperativity (alpha, relative to Kd for VH032) = 18. Data from ref. (1)

Proteome-wide selectivity: Unbiased and quantitative isobaric tagging MS proteomics confirmed no proteins other than VHL and Cul2 as substantially depleted upon treatment of HeLa cells with CM11 (1 uM for 10 h) amongst the 6,450 detected proteins that passed filtering criteria, compared to DMSO or VHL inhibitor VH032 treatment. Data from ref. (1)

In vivo PK data: not available

Crystal Structure: not available

Negative control: CMP98, CAS number: 2244684-50-0, available from Tocris


Primary References: 

  1. Maniaci et al. (2017) Homo-PROTACs: Bivalent small-molecule dimerizers of the VHL E3 ubiquitin ligase to induce self-degradation. Nat. Commun. 8, 830.  DOI: 10.1038/s41467-017-00954-1; PMID: 29018234


Articles that have used CM11:

  1. 2017 Maniaci Nat Commun
  2. 2019 Steinebach Chem Commun
  3. 2019 Girardini Bioorg Med Chem
  4. 2020 Klein ACS Med Chem Lett
  5. 2021 Castro RSC Med Chem
  6. 2022 Gui ChemBioChem
  7. 2022 Trapotsi ACS Chem Biol
  8. 2023 Gui ACS Chem Biol
  9. 2023 Gui ChemBioChem


More information on our chemical probes can be found from the Chemical Probes portal, and from commercial vendors such as Tocris Bio-Techne.