The School of Life Sciences at the University of Dundee is a world-class academic institution with a reputation for the excellence of its research, its high-quality teaching and student experience, and the strong impact of its activities outside academia. With 900 staff from over 60 countries worldwide the School provides a dynamic, multi-national, collegiate and diverse environment with state-of-the-art laboratory, technology and teaching facilities.
Applications are invited for a position as a Research Technician in the laboratory of Dr. Federico Pelisch, to investigate the role of post-translational modifications during chromosome segregation in oocytes.
Most chromosomal abnormalities in human embryos arise after losing or gaining one or more chromosomes during meiosis. Aneuploid embryos account for at least 10% of human pregnancies and, for women nearing the end of their reproductive lifespan, the incidence may exceed 50%. In spite of this, little is known about how meiotic chromosome segregation is regulated. The broader aim of this project is to combine biochemistry with in vivo methodologies to understand how this regulation is achieved in vivo at the mechanistic level. Biochemical methods include protein expression and purification in bacteria and insect cells; in vitro interaction methodologies such as fluorescence polarisation, pull-downs, and ITC, sumoylation and phosphorylation assays, amongst others. In vivo methodologies will include live cell imaging of dissected oocytes and whole worms, immunofluorescence, CRISPR/Cas genome editing, and also mass spectrometry-based proteomics. Research in the Pelisch lab is funded by a prestigious Medical Research Council Fellowship. For more information, you can visit the lab’s website at www.pelischlab.co.uk or Twitter @fpelisch.
You will have access to state-of-the-art technologies in biochemistry, microscopy, and proteomics. We therefore provide an excellent environment for studies using in vitro systems as well as validation of newly discovered mechanisms using in vivo models. As such, the position is well-suited for candidates with a strong interest in basic biological science that also wish to work on research projects with a relevance in cell division in health and disease, particularly related to fertility.
You will have the opportunity to enrol as a part-time PhD student at University of Dundee.
Who we are looking for:
• Must hold (or anticipate to receive) a graduate degree (honours of the first or second class)/ Masters or equivalent
• Self-motivated individual with a curious mind-set interested in taking a lead role in a research project
• Strong interest in chromatin and epigenetics research
• Basic skills in molecular biology and biochemistry are essential. Experience with C. elegans handling including crossing, genotyping, etc, and/or microscopy are desirable.
• Good knowledge of the English language is essential.
To apply for this position, please submit your cover letter and CV, including the contact information (name, institution, and e-mail address) of 2 referees. We encourage applications both from UK and the European Economic Area (EEA).
For informal enquiries about this position please contact Federico Pelisch at firstname.lastname@example.org. The Pelisch lab is based in the Centre for Gene Regulation & Expression (GRE) within the School of Life Sciences in Dundee. To find out more please visit http://gre.dundee.ac.uk.
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