Faithful chromosome segregation requires all connections that physically link chromosomes, to be removed before cells divide; failure to do so can prevent cell division or cause aneuploidy and disease. Prior to segregation, proteinaceous connections between chromosomes, such as cohesins must be removed, but so must DNA structures that link chromatids. These include DNA repair intermediates such as Holliday Junctions, points at which recombined chromatids have become inter-twined, and loci that have not replicated by the time cells reach the metaphase-anaphase transition.
Using C. elegans genetic and cell biological approaches we defined a new mechanism for the detection of chromatid linkages that escape detection and processing in late S/early M phase and thus persist into telophase. This mechanism involves the processing of persistent DNA structures by the previously uncharacterized nuclease LEM-3. This nuclease appears to acts at the midbody just before cells divide.
As part of this 3 year BBSRC funded project we aim to address which DNA structures are processed by LEM-3, how LEM-3 is regulated, and if there is a role of the vertebrate in genome maintenance.
The project will be conducted in collaboration with Prof. David Lilley and John Rouse.
Applicants must have a PhD in molecular biology, genetics, biochemistry or a related subject. For the first position the candidate should ideally have experience with the purification of recombinant proteins, as well as techniques relating to handling mammalian cells. The candidate should have a keen interest in the DNA repair filed and will be co-advised by Profs David Lilley and John Rouse. The second position requires expertise in C. elegans genetics and cell biology. Ideally the candidate should have expertise in real time imaging, advanced microscopy and C. elegans genome editing, but all outstanding candidates will be considered. Applicants should have excellent written and spoken English communication skills and should be able to work both independently and as part of a team.
Appointment as a Postdoctoral Research Assistant on the Grade 7 salary scale is dependent upon you having been awarded a PhD. An appointment may be considered if you are expected to be awarded a PhD (Spinal Point 28, £31,302).
For informal enquiries please contact Professor Anton Gartner (firstname.lastname@example.org)
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