DogTag – a genetically encoded proximity labelling strategy to capture problematic protein-protein interactions
Applications are invited for a 2 year post-doctoral position funded by the BBSRC. We are looking for an enthusiastic and motivated post-doctoral scientist to join our team and develop a novel strategy for mapping protein-protein interactions.
Capturing protein-protein interactions is key to understanding biological processes yet many interactions are missed due to being weak, transient or not amenable to heterologous or biochemical examination. There are many existing strategies to investigate protein-protein interactions (e.g. BioID, Y-2-H, IP-MS) but none allow for naïve, non-invasive, in-vivo examination of weak or transient protein interactions under physiologically relevant conditions. We are piloting a novel genetically encoded method allowing for proximity labelling experiments to be performed unaffected by these limitations. The aim of the project is to develop the method and ultimately perform differential discovery proteomic screens to identify enzyme-substrate interactions in whole organisms under biologically relevant stimuli.
The appointee will be responsible for project implementation and will be involved in the day-to-day supervision of a PhD student on a related project. Strong candidates for incoming post-doctoral fellowships (e.g. Marie Curie) are especially welcome to apply.
Appointment as a Postdoctoral Research Assistant on the Grade 7 salary scale is dependent upon you having been awarded a PhD. An appointment may be considered if you are shortly expected to be awarded a PhD. The initial appointment will be made as a Research Assistant on the Training Grade 7 salary scale (Spinal Point 28, £30,688).
Summary of Skills, Experience and Qualifications
Applicants should have, or be about to obtain, a PhD, and have a solid background in molecular biology and biochemistry. Experience in proteomic experimental planning and data analysis is essential. Experience of methods development, working with membrane proteins, protein biochemistry, proximity labelling screens and proteomic sample processing would be advantageous.
College / School / Directorate Information
The position is in the laboratory of Dr. Piers Hemsley at the University of Dundee (College of Life Sciences; Division of Plant Sciences - http://www.lifesci.dundee.ac.uk/ps) based at the James Hutton Institute. Dundee (http://www.lifesci.dundee.ac.uk/) is currently ranked as the #1 University in the UK for life science research by the UK government research assessment (REF 2014) with world leading expertise in cell signalling and proteomics and outstanding laboratory and technology facilities for fundamental research and translation. With more than 900 staff from over 60 countries worldwide, the College of Life Sciences enjoys an international reputation as one of the most vibrant and productive research centres in Europe and is frequently voted “the best place to work in Europe” by readers of The Scientist magazine. The James Hutton Institute is the largest plant breeding and research institute in Scotland with particular expertise in translational plant science.
This post is available for up to 24 months, latest start date 3rd September 2018.
Informal enquiries are encouraged: Dr Piers Hemsley, e-mail: email@example.com
The University of Dundee is a diverse community and is committed to equality of opportunity for all by providing a supportive, flexible and inclusive working environment. We have family friendly policies (including flexible working and childcare vouchers), staff groups for LGBT and BME; and prayer room facilities.
The University of Dundee has received the Athena SWAN Bronze Award (Athena Swan) which recognises the promotion of gender equality and has made further commitment to advancing inclusive culture which supports and encourages all under-represented groups.
The University of Dundee is a Scottish Registered Charity, No. SC015096.