The Drug Discovery Unit (www.drugdiscovery.dundee.ac.uk) is a dynamic and multidisciplinary group of 85 scientists with expertise in compound handling, assay development, high-throughput screening, medicinal and computational chemistry and in vitro and in vivo pharmacokinetics. The unit is housed in a state-of-the-art building and has cutting-edge equipment and automation for hit-discovery and lead optimisation.
We are seeking an experienced and motivated protein purification scientist/ crystallographer to help expand our protein production capabilities and support structure based drug discovery projects. Protein production is carried out using a variety of different expression systems within our dedicate protein production team (http://www.lifesci.dundee.ac.uk/services/bcdd-protein-production). The role will involve construct design and development and optimisation of methods and protocols for protein production. The role encompasses all aspects of crystallography and will involve generating ligand-protein structures as part of structure-based drug discovery project and novel protein structures. The role holder will be expected to contribute to current and future structure based drug discovery projects. The successful candidate should have a high level of technical expertise in all aspects of protein scale up and protein crystallography. Knowledge of drug discovery is desirable but not essential
• Development and validation of proteins production protocols as well as supplying drug discovery projects (50%)
• Direct crystallography support for structure based drug discovery projects (50%)
• A track record in all aspects of protein engineering from construct design, production, purification and validation
• Hands on experience of protein crystallography with a demonstrable understanding of the range of protein constructs desired/required to support projects
• Excellent problem solving and creative thinking abilities
• Good interpersonal and leadership skills
• The ability to work in teams, and to work to timelines and deliver milestones
• Self-motivated, able to work independently with minimal direction/oversight
• To design, clone, express and purify proteins in a variety of expression systems (E.coli, Baculovirus, P.pastoris and mammalian).
• all aspects of crystallography but has a particular emphasis on novel protein structures and protein/crystal co-complexes
• To seek and maintain high working standards in both laboratory and paperwork.
• Provide prompt and accurate communication with customers.
• Provide high quality and signed off reports to customers when required.
• Keeping up to date in new technologies that may improve PPT service/success.
• Working collaboratively with members of the Drug Discovery Unit and academic or industrial collaborators to support protein production.
• A first or second degree in biochemistry, or equivalent experience.
• An ability to be proactive, productive and to work effectively in teams is required.
• Strong interpersonal, communication and presentation skills.
• A demonstrated ability to work in an organised and meticulous manner and to standard operating procedures is essential.
• Experience of expression and purification of recombinant proteins is essential.
• Construct Design and Molecular Biology skills are essential.
• Experience of Tissue Culture would be an advantage.
• Use of AKTA purification systems is essential.
• Experience of the use of Bioreactors would be an advantage.
How to apply:
To apply on-line click here.
The University of Dundee is a diverse community and is committed to equality of opportunity for all by providing a supportive, flexible and inclusive working environment. We have family friendly policies (including flexible working and childcare vouchers), staff groups for LGBT and BME; and prayer room facilities.
The University of Dundee has received the Athena SWAN Bronze Award (http://www.dundee.ac.uk/about/athenaswan/) which recognises the promotion of gender equality and has made further commitment to advancing inclusive culture which supports and encourages all under-represented groups.
The University of Dundee is a Scottish Registered Charity, No. SC015096.