University of Dundee

MRC Protein Phosphorylation Unit 2018

Alessi DR, Sammler E. 2018. LRRK2 kinase in Parkinson's disease. Science. 360(6384):36-37. https://doi.org/10.1126/science.aar5683

Barini E, Miccoli A, Tinarelli F, Mulholland K, Kadri H, Khanim F, Stojanovski L, Read KD, Burness K, Blow JJ, Mehellou Y, Muqit MMK. 2018. The Anthelmintic Drug Niclosamide and Its Analogues Activate the Parkinson's Disease Associated Protein Kinase PINK1. ChemBioChem. 19(5):425-429. https://doi.org/10.1002/cbic.201700500

Bozatzi P, Dingwell KS, Wu KZL, Cooper F, Cummins TD, Hutchinson LD, Vogt J, Wood NT, Macartney TJ, Varghese J, Gourlay R, Campbell DG, Smith JC, Sapkota GP. 2018. PAWS1 controls Wnt signalling through association with casein kinase 1α. EMBO Reports. 19(4):1-18. https://doi.org/10.15252/embr.201744807

Bozatzi P, Sapkota G. 2018. The FAM83 family of proteins: from pseudo-PLDs to anchors for CK1 isoforms. Biochemical Society Transactions. 2018:761-771. https://doi.org/10.1042/BST20160277

Bustos F, Segarra Fas A, Chaugule VK, Brandenburg L, Branigan E, Toth R, Macartney T, Knebel A, Hay R, Walden H, Findlay G. 2018. RNF12 X-linked intellectual disability mutations disrupt E3 ligase activity and neural differentiation. Cell Reports. 23:1599-1611. https://doi.org/10.1016/j.celrep.2018.04.022

Cohen P. 2018. Ubiquitin chains as second messengers. Nature Reviews Molecular Cell Biology. 19. https://doi.org/10.1038/nrm.2018.9

Cummins TD, Wu KZL, Bozatzi P, Dingwell KS, Macartney TJ, Wood NT, Varghese J, Gourlay R, Campbell DG, Prescott A, Griffis E, Smith JC, Sapkota GP. 2018. PAWS1controls cytoskeletal dynamics and cell migration through association with the SH3 adaptor CD2AP. Journal of Cell Science. 131(1):1-12. https://doi.org/Final published version, https://doi.org/10.1242/jcs.202390

Cummins TD, Sapkota GP. 2018. Characterization of Protein Complexes Using Chemical Cross-Linking Coupled Electrospray Mass Spectrometry. Sarwal M, Sigel T, editors. In Tissue Proteomics: Methods and Protocols. New York: Humana Press. pp. 43-61. (Methods in Molecular Biology (Clifton, N.J.)). https://doi.org/10.1007/7651_2017_85

Dhekne HS, Yanatori I, Gomez RC, Tonelli F, Diez F, Schüle B, Steger M, Alessi DR, Pfeffer SR. 2018. A pathway for Parkinson's Disease LRRK2 kinase to block primary cilia and Sonic hedgehog signaling in the brain. eLife. 7. https://doi.org/10.7554/eLife.40202

Di Maio R, Hoffman EK, Rocha EM, Keeney MT, Sanders LH, De Miranda BR, Zharikov A, Van Laar A, Stepan AF, Lanz TA, Kofler JK, Burton EA, Alessi DR, Hastings TG, Timothy Greenamyre J. 2018. LRRK2 activation in idiopathic Parkinson's disease. Science Translational Medicine. 10(451). https://doi.org/10.1126/scitranslmed.aar5429

Evrin C, Maman J, Diamante A, Pellegrini L, Labib K. 2018. Histone H2A-H2B binding by Pol α in the eukaryotic replisome contributes to the maintenance of repressive chromatin. EMBO Journal. 37(16):1-16. https://doi.org/10.15252/embj.201899021

Fan Y, Howden AJM, Sarhan AR, Lis P, Ito G, Martinez TN, Brockmann K, Gasser T, Alessi DR, Sammler E. 2018. Interrogating Parkinson's disease LRRK2 kinase pathway activity by assessing Rab10 phosphorylation in human neutrophils. Biochemical Journal. 475(1):23-44. https://doi.org/10.1042/BCJ20170803

Fulcher L, Bozatzi P, Tachie-Menson T, Wu K, Cummins TD, Bufton JC, Pinkas DM, Dunbar K, Shrestha S, Wood N, Weidlich S, Macartney T, Varghese J, Gourlay R, Campbell D, Dingwell KS, Smith JC, Bullock AN, Sapkota G. 2018. The DUF1669 domain of FAM83 family proteins anchor casein kinase 1 isoforms. Science Signaling. 11(531). https://doi.org/10.1126/scisignal.aao2341

Gan H, Serra-Cardona A, Hua X, Zhou H, Labib K, Yu C, Zhang Z. 2018. The Mcm2-Ctf4-Polα Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands. Molecular Cell. 72(1):140-151.e3. https://doi.org/10.1016/j.molcel.2018.09.001

Ganley IG. 2018. Organelle Turnover: A USP30 Safety Catch Restrains the Trigger for Mitophagy and Pexophagy. Current Biology. 28(15):R842-R845. https://doi.org/10.1016/j.cub.2018.06.067

Graciela Cervantes-Perez L, Castaneda-Bueno M, Jimenez JV, Vazquez N, Rojas-Vega L, Alessi D, Bobadilla NA, Gamba G. 2018. Disruption of the with no lysine kinase^STE20-proline alanine-rich kinase pathway reduces thehypertension induced by angiotensin II. Journal of Hypertension. 36(2):361-367. https://doi.org/10.1097/HJH.0000000000001554

Härtlova A, Herbst S, Peltier J, Rodgers A, Bilkei-Gorzo O, Fearns A, Dill BD, Lee H, Flynn R, Cowley SA, Davies P, Lewis PA, Ganley IG, Martinez J, Alessi DR, Reith AD, Trost M, Gutierrez MG. 2018. LRRK2 is a negative regulator of phagosome maturation in macrophages. The EMBO Journal. 2018:1-17. https://doi.org/10.15252/embj.201798694

Hong Y, Sonneville R, Wang B, Scheidt V, Meier B, Woglar A, Demetriou S, Labib K, Jantsch V, Gartner A. 2018. LEM-3 is a midbody-tethered DNA nuclease that resolves chromatin bridges during late mitosis. Nature Communications. 9:1-11. https://doi.org/10.1038/s41467-018-03135-w

Hong Y, Velkova M, Silva N, Jagut M, Scheidt V, Labib K, Jantsch V, Gartner A. 2018. The conserved LEM-3/Ankle1 nuclease is involved in the combinatorial regulation of meiotic recombination repair and chromosome segregation in Caenorhabditis elegans. PLoS Genetics. 14(6):1-22. https://doi.org/10.1371/journal.pgen.1007453

Hutchinson LD, Bozatzi P, Macartney T, Sapkota GP. 2018. Generation of endogenous BMP transcriptional reporter cells through CRISPR/Cas9 genome editing. Rogers MB, editor. In Bone Morphogenetic Proteins: Methods and Protocols. New York: Humana Press. pp. 29-35. (Methods in Molecular Biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-4939-8904-1_4

Jowsey P, Morrice NA, Hastie CJ, MacLauchlan H, Toth R, Rouse J. 2018. Corrigendum to "Characterisation of the sites of DNA damage-induced 53BP1 phosphorylation catalysed by ATM and ATR" [DNA Repair 6 (2007) 1536-1544]. DNA Repair. 65:80. https://doi.org/10.1016/j.dnarep.2017.10.012

Kelsall IR, Kristariyanto YA, Knebel A, Wood NT, Kulathu Y, Alpi AF. 2018. Coupled monoubiquitylation of the co-E3 ligase DCNL1 by Ariadne RBR E3 ubiquitin ligases promotes cullin-RING ligase complex remodeling. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.RA118.005861

Kulathu Y, Zuern C, Yang J, Reth M. 2018. Synthetic biology of B cell activation: understanding signal amplification at the B cell antigen receptor using a rebuilding approach. Biological Chemistry. https://doi.org/10.1515/hsz-2018-0308

Kunath T, Natalwala A, Chan C, Chen Y, Stecher B, Taylor M, Khan S, Muqit MMK. 2018. Are PARKIN patients ideal candidates for dopaminergic cell replacement therapies?. European Journal of Neuroscience. https://doi.org/10.1111/ejn.14314

Kwasna D, Rehman SAA, Natarajan J, Matthews S, Madden R, De Cesare V, Weidlich S, Virdee S, Ahel I, Gibbs-Seymour I, Kulathu Y. 2018. Discovery and characterization of ZUFSP/ZUP1, a distinct DUB class important for genome stability. Molecular Cell. 70:1-15. https://doi.org/10.1016/j.molcel.2018.02.023

Leznicki P, Natarajan J, Bader G, Spevak W, Schlattl A, Rehman SAA, Pathak D, Weidlich S, Zoephel A, Bordone MC, Barbosa-Morais NL, Boehmelt G, Kulathu Y. 2018. Expansion of DUB functionality by alternative isoforms: USP35, a case study . Journal of Cell Science. 131. https://doi.org/10.1242/jcs.212753

Lis P, Burel S, Steger M, Mann M, Brown F, Diez F, Tonelli F, Holton JL, Winglok Ho P, Ho S-L, Chou M-Y, Polinski NK, Martinez TN, Davies P, Alessi DR. 2018. Development of phospho-specific Rab protein antibodies to monitor in vivo activity of the LRRK2 Parkinson’s disease kinase. Biochemical Journal. 475(1):1-22. https://doi.org/10.1042/BCJ20170802

Malik N, Macartney T, Hornberger A, Anderson K, Tovell H, Prescott AR, Alessi DR. 2018. Mechanism of activation of SGK3 by growth factors-via the Class 1 and Class 3 PI3Ks. Biochemical Journal. 475(1):117-135. https://doi.org/10.1042/BCJ20170650

McWilliams TG, Prescott AR, Montava-Garriga L, Ball G, Singh F, Barini E, Muqit MMK, Brooks SP, Ganley IG. 2018. Basal Mitophagy Occurs Independently of PINK1 in Mouse Tissues of High Metabolic Demand. Cell Metabolism. 27(2):439-449.e5. https://doi.org/10.1016/j.cmet.2017.12.008

McWilliams TG, Barini E, Pohjolan-Pirhonen R, Brooks SP, Singh F, Burel S, Balk K, Kumar A, Montava Garriga L, Prescott A, Hassoun SM, Mouton-Liger F, Ball G, Hills R, Knebel A, Ulusoy A, Di Monte DA, Tamjar J, Antico O, Fears K, Smith L, Brambilla R, Palin E, Valori M, Eerola-Rautio J, Tienari P, Corti O, Dunnett SB, Ganley I, Suomalainen A, Muqit M. 2018. Phosphorylation of Parkin at Serine65 is essential for its activation in vivo. Open Biology. 8(11). https://doi.org/10.1098/rsob.180108

Mir R, Tonelli F, Lis P, Macartney T, Polinski NK, Martinez TN, Chou M-Y, Howden A, König T, Hotzy C, Milenkovic I, Brücke T, Zimprich A, Sammler E, Alessi D. 2018. The Parkinson's disease VPS35[D620N] mutation enhances LRRK2 mediated Rab protein phosphorylation in mouse and human. Biochemical Journal. 475(11):1861-1883. https://doi.org/10.1042/BCJ20180248

Munoz I, Morgan M, Peltier J, Weiland F, Gregorczyk M, Brown FCM, Macartney T, Toth R, Trost M, Rouse J. 2018. Phosphoproteomic screening identifies physiological substrates of the CDKL5 kinase. The EMBO Journal. 37(19):1-19. https://doi.org/10.15252/embj.201899559

Nanda SK, Nagamori T, Windheim M, Amu S, Aviello G, Patterson-Kane J, Arthur JSC, Ley SC, Fallon P, Cohen P. 2018. ABIN2 Function Is Required To Suppress DSS-Induced Colitis by a Tpl2-Independent Mechanism. Journal of Immunology. 201(11):3373-3382. https://doi.org/10.4049/jimmunol.1700614

Osborne J, Birchall K, Tsagris DJ, Lewis SJ, Smiljanic-Hurley E, Taylor DL, Levy A, Alessi D, McIver EG. 2018. Discovery of potent and selective 5-azaindazole inhibitors of leucine-rich repeat kinase 2 (LRRK2): Part 1. Bioorganic & Medicinal Chemistry Letters. https://doi.org/10.1016/j.bmcl.2018.11.058

Pao K-C, Wood NT, Knebel A, Rafie K, Stanley M, Mabbitt PD, Sundaramoorthy R, Hofmann K, van Aalten DMF, Virdee S. 2018. Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity. Nature. 556(7701):381-385. https://doi.org/10.1038/s41586-018-0026-1

Ramachandran A, Vizan P, Das D, Chakravarty P, Vogt J, Rogers KW, Müller P, Hinck AP, Sapkota GP, Hill CS. 2018. TGF-β uses a novel mode of receptor activation to phosphorylate SMAD1/5 and induce epithelial-to-mesenchymal transition. eLife. 7:1-29. https://doi.org/10.7554/eLife.31756

Rousseau A, Bertolotti A. 2018. Regulation of proteasome assembly and activity in health and disease. Nature Reviews Molecular Cell Biology. 19:697-712. https://doi.org/10.1038/s41580-018-0040-z

Thomas Y, Scott DC, Kristariyanto YA, Rinehart J, Clark K, Cohen P, Kurz T. 2018. The NEDD8 E3 ligase DCNL5 is phosphorylated by IKK alpha during Toll-like receptor activation. PLoS ONE. 13(6):1-19. https://doi.org/10.1371/journal.pone.0199197

Vagnarelli P, Alessi DR. 2018. PP1 Phosphatase complexes: no longer undruggable enzymes. Cell. 174(5):1049-1051. https://doi.org/10.1016/j.cell.2018.08.007

Wan X, Serrill JD, Humphreys IR, Tan M, McPhail KL, Ganley IG, Ishmael JE. 2018. ATG5 Promotes Death Signaling in Response to the Cyclic Depsipeptides Coibamide A and Apratoxin A. Marine Drugs. 16(3):1-20. https://doi.org/10.3390/md16030077

Zhao Y, Keshiya S, Atashrazm F, Gao J, Ittner LM, Alessi DR, Halliday GM, Fu Y, Dzamko N. 2018. Nigrostriatal pathology with reduced astrocytes in LRRK2 S910/S935 phosphorylation deficient knockin mice. Neurobiology of Disease. 120:76-87. https://doi.org/10.1016/j.nbd.2018.09.003