University of Dundee

MRC Protein Phosphorylation Unit 2008

Al-Hakim, A. K., Zagorska, A., Chapman, L., Deak, M., Peggie, M. and Alessi, D. R. (2008) Control of AMPK-related kinases by USP9X and atypical Lys(29)/Lys(33)-linked polyubiquitin chains. Biochem J. 411, 249-260

Ananieva, O., Darragh, J., Johansen, C., Carr, J. M., McIlrath, J., Park, J. M., Wingate, A., Monk, C. E., Toth, R., Santos, S. G., Iversen, L. and Arthur, J. S. (2008) The kinases MSK1 and MSK2 act as negative regulators of Toll-like receptor signaling. Nat Immunol. 9, 1028-1036

Ananieva, O., Macdonald, A., Wang, X., McCoy, C. E., McIlrath, J., Tournier, C. and Arthur, J. S. (2008) ERK5 regulation in naive T-cell activation and survival. Eur J Immunol. 38, 2534-2547

Arthur, J. S. (2008) MSK activation and physiological roles. Front Biosci. 13, 5866-5879

Bayascas, J. R., Wullschleger, S., Sakamoto, K., Garcia-Martinez, J. M., Clacher, C., Komander, D., van Aalten, D. M., Boini, K. M., Lang, F., Lipina, C., Logie, L., Sutherland, C., Chudek, J. A., van Diepen, J. A., Voshol, P. J., Lucocq, J. M. and Alessi, D. R. (2008) Mutation of the PDK1 PH domain inhibits protein kinase B/Akt, leading to small size and insulin resistance. Mol Cell Biol. 28, 3258-3272

Belgardt, B. F., Husch, A., Rother, E., Ernst, M. B., Wunderlich, F. T., Hampel, B., Klockener, T., Alessi, D., Kloppenburg, P. and Bruning, J. C. (2008) PDK1 deficiency in POMC-expressing cells reveals FOXO1-dependent and -independent pathways in control of energy homeostasis and stress response. Cell Metab. 7, 291-301

Bouskila, M., Hirshman, M. F., Jensen, J., Goodyear, L. J. and Sakamoto, K. (2008) Insulin promotes glycogen synthesis in the absence of GSK3 phosphorylation in skeletal muscle. Am J Physiol Endocrinol Metab. 294, E28-35

Buxade, M., Morrice, N., Krebs, D. L. and Proud, C. G. (2008) The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha. J Biol Chem. 283, 57-65

Carroll, A. M., Porter, R. K. and Morrice, N. A. (2008) Identification of serine phosphorylation in mitochondrial uncoupling protein 1. Biochim Biophys Acta. 1777, 1060-1065

Chandramohan, Y., Droste, S. K., Arthur, J. S. and Reul, J. M. (2008) The forced swimming-induced behavioural immobility response involves histone H3 phospho-acetylation and c-Fos induction in dentate gyrus granule neurons via activation of the N-methyl-D-aspartate/extracellular signal-regulated kinase/mitogen- and stress-activated kinase signalling pathway. Eur J Neurosci. 27, 2701-2713

Chen, S., Murphy, J., Toth, R., Campbell, D. G., Morrice, N. A. and MacKintosh, C. (2008) Complementary regulation of TBC1D1 and AS160 by growth factors, insulin and AMPK activators. Biochem J. 409, 449-459

Clark, K., Middelbeek, J., Lasonder, E., Dulyaninova, N. G., Morrice, N. A., Ryazanov, A. G., Bresnick, A. R., Figdor, C. G. and van Leeuwen, F. N. (2008) TRPM7 regulates myosin IIA filament stability and protein localization by heavy chain phosphorylation. J Mol Biol. 378, 788-801

Clark, K., Middelbeek, J., Morrice, N. A., Figdor, C. G., Lasonder, E. and van Leeuwen, F. N. (2008) Massive autophosphorylation of the Ser/Thr-rich domain controls protein kinase activity of TRPM6 and TRPM7. PLoS ONE. 3, e1876

Cole, A. R., Soutar, M. P., Rembutsu, M., van Aalten, L., Hastie, C. J., McLauchlan, H., Peggie, M., Balastik, M., Lu, K. P. and Sutherland, C. (2008) Relative Resistance of Cdk5-phosphorylated CRMP2 to Dephosphorylation. J Biol Chem. 283, 18227-18237

Cuomo, M. E., Knebel, A., Morrice, N., Paterson, H., Cohen, P. and Mittnacht, S. (2008) p53-Driven apoptosis limits centrosome amplification and genomic instability downstream of NPM1 phosphorylation. Nat Cell Biol. 10, 723-730

Duro, E., Vaisica, J. A., Brown, G. W. and Rouse, J. (2008) Budding yeast Mms22 and Mms1 regulate homologous recombination induced by replisome blockage. DNA Repair (Amst). 7, 811-818

Filippi, B. M. and Alessi, D. R. (2008) Novel role for the LKB1 pathway in controlling monocarboxylate fuel transporters. J Cell Biol. 183, 7-9

Garcia-Martinez, J. M. and Alessi, D. R. (2008) mTOR complex 2 (mTORC2) controls hydrophobic motif phosphorylation and activation of serum- and glucocorticoid-induced protein kinase 1 (SGK1). Biochem J. 416, 375-385

Gardiner, M., Toth, R., Vandermoere, F., Morrice, N. A. and Rouse, J. (2008) Identification and characterization of FUS/TLS as a new target of ATM. Biochem J. 415, 297-307

Gohler, T., Munoz, I. M., Rouse, J. and Blow, J. J. (2008) PTIP/Swift is required for efficient PCNA ubiquitination in response to DNA damage. DNA Repair (Amst). 7, 775-787

Green, C. J., Goransson, O., Kular, G. S., Leslie, N. R., Gray, A., Alessi, D. R., Sakamoto, K. and Hundal, H. S. (2008) Use of Akt Inhibitor and a Drug-resistant Mutant Validates a Critical Role for Protein Kinase B/Akt in the Insulin-dependent Regulation of Glucose and System A Amino Acid Uptake. J Biol Chem. 283, 27653-27667

Holman, G. D. and Sakamoto, K. (2008) Regulating the motor for GLUT4 vesicle traffic. Cell Metab. 8, 344-346

Howlett, K. F., Mathews, A., Garnham, A. and Sakamoto, K. (2008) The effect of exercise and insulin on AS160 phosphorylation and 14-3-3 binding capacity in human skeletal muscle. Am J Physiol Endocrinol Metab. 294, E401-407

Huang, X., Wullschleger, S., Shpiro, N., McGuire, V. A., Sakamoto, K., Woods, Y. L., McBurnie, W., Fleming, S. and Alessi, D. R. (2008) Important role of the LKB1-AMPK pathway in suppressing tumorigenesis in PTEN-deficient mice. Biochem J. 412, 211-221

Hur, W., Velentza, A., Kim, S., Flatauer, L., Jiang, X., Valente, D., Mason, D. E., Suzuki, M., Larson, B., Zhang, J., Zagorska, A., Didonato, M., Nagle, A., Warmuth, M., Balk, S. P., Peters, E. C. and Gray, N. S. (2008) Clinical stage EGFR inhibitors irreversibly alkylate Bmx kinase. Bioorg Med Chem Lett. 18, 5916-5919

Kim, C., Sano, Y., Todorova, K., Carlson, B. A., Arpa, L., Celada, A., Lawrence, T., Otsu, K., Brissette, J. L., Arthur, J. S. and Park, J. M. (2008) The kinase p38 alpha serves cell type-specific inflammatory functions in skin injury and coordinates pro- and anti-inflammatory gene expression. Nat Immunol. 9, 1019-1027

Koshinaka, K., Sano, A., Howlett, K. F., Yamazaki, T., Sasaki, M., Sakamoto, K. and Kawanaka, K. (2008) Effect of high-intensity intermittent swimming on postexercise insulin sensitivity in rat epitrochlearis muscle. Metabolism. 57, 749-756

Martin-Granados, C., Philp, A., Oxenham, S. K., Prescott, A. R. and Cohen, P. T. W. (2008) Depletion of protein phosphatase 4 in human cells reveals essential roles in centrosome maturation, cell migration and the regulation of Rho GTPases. Int J Biochem Cell Biol. 40, 2315-2332

McCracken, S. R., Ramsay, A., Heer, R., Mathers, M. E., Jenkins, B. L., Edwards, J., Robson, C. N., Marquez, R., Cohen, P. and Leung, H. Y. (2008) Aberrant expression of extracellular signal-regulated kinase 5 in human prostate cancer. Oncogene. 27, 2978-2988

Mendoza, H., Campbell, D. G., Burness, K., Hastie, J., Ronkina, N., Shim, J. H., Arthur, J. S., Davis, R. J., Gaestel, M., Johnson, G. L., Ghosh, S. and Cohen, P. (2008) Roles for TAB1 in regulating the IL-1-dependent phosphorylation of the TAB3 regulatory subunit and activity of the TAK1 complex. Biochem J. 409, 711-722

Moorhead, G. B., Trinkle-Mulcahy, L., Nimick, M., De Wever, V., Campbell, D. G., Gourlay, R., Lam, Y. W. and Lamond, A. I. (2008) Displacement affinity chromatography of protein phosphatase one (PP1) complexes. BMC Biochem. 9, 28

Morin, I., Ngo, H. P., Greenall, A., Zubko, M. K., Morrice, N. and Lydall, D. (2008) Checkpoint-dependent phosphorylation of Exo1 modulates the DNA damage response. Embo J. 27, 2400-2410

Morton, S., Hesson, L., Peggie, M. and Cohen, P. (2008) Enhanced binding of TBK1 by an optineurin mutant that causes a familial form of primary open angle glaucoma. FEBS Lett. 582, 997-1002

Ordureau, A., Smith, H., Windheim, M., Peggie, M., Carrick, E., Morrice, N. and Cohen, P. (2008) The IRAK-catalysed activation of the E3 ligase function of Pellino isoforms induces the Lys63-linked polyubiquitination of IRAK1. Biochem J. 409, 43-52

Pagano, M. A., Bain, J., Kazimierczuk, Z., Sarno, S., Ruzzene, M., Di Maira, G., Elliott, M., Orzeszko, A., Cozza, G., Meggio, F. and Pinna, L. A. (2008) The selectivity of inhibitors of protein kinase CK2: an update. Biochem J. 415, 353-365

Paterson, J., Kelsall, I. R. and Cohen, P. T. W. (2008) Disruption of the striated muscle glycogen-targeting subunit of protein phosphatase 1: influence of the genetic background. J Mol Endocrinol. 40, 47-59

Paytubi, S., Morrice, N. A., Boudeau, J. and Proud, C. G. (2008) The N-terminal region of ABC50 interacts with eukaryotic initiation factor eIF2 and is a target for regulatory phosphorylation by CK2. Biochem J. 409, 223-231
Peifer, C. and Alessi, D. R. (2008) Small-molecule inhibitors of PDK1. ChemMedChem. 3, 1810-1838

Rembutsu, M., Soutar, M. P., Van Aalten, L., Gourlay, R., Hastie, C. J., McLauchlan, H., Morrice, N. A., Cole, A. R. and Sutherland, C. (2008) Novel procedure to investigate the effect of phosphorylation on protein complex formation in vitro and in cells. Biochemistry. 47, 2153-2161
 Richardson, C. and Alessi, D. R. (2008) The regulation of salt transport and blood pressure by the WNK-SPAK/OSR1 signalling pathway. J Cell Sci. 121, 3293-3304

Richardson, C., Rafiqi, F. H., Karlsson, H. K., Moleleki, N., Vandewalle, A., Campbell, D. G., Morrice, N. A. and Alessi, D. R. (2008) Activation of the thiazide-sensitive Na+-Cl- cotransporter by the WNK-regulated kinases SPAK and OSR1. J Cell Sci. 121, 675-684

Rousseau, S., Papoutsopoulou, M., Symons, A., Cook, D., Lucocq, J. M., Prescott, A. R., O'Garra, A., Ley, S. C. and Cohen, P. (2008) TPL2-mediated activation of ERK1 and ERK2 regulates the processing of pre-TNF{alpha} in LPS-stimulated macrophages. J Cell Sci. 121, 149-154

Sakamoto, K. (2008) Silencing metabolic disorders by novel SIRT1 activators. Cell Metab. 7, 3-4

Sakamoto, K. and Holman, G. D. (2008) Emerging role for AS160/TBC1D4 and TBC1D1 in the regulation of GLUT4 traffic. Am J Physiol Endocrinol Metab. 295, E29-37

Spycher, C., Miller, E. S., Townsend, K., Pavic, L., Morrice, N. A., Janscak, P., Stewart, G. S. and Stucki, M. (2008) Constitutive phosphorylation of MDC1 physically links the MRE11-RAD50-NBS1 complex to damaged chromatin. J Cell Biol. 181, 227-240

Towler, M. C., Fogarty, S., Hawley, S. A., Pan, D. A., Martin, D. M., Morrice, N. A., McCarthy, A., Galardo, M. N., Meroni, S. B., Cigorraga, S. B., Ashworth, A., Sakamoto, K. and Hardie, D. G. (2008) A novel short splice variant of the tumour suppressor LKB1 is required for spermiogenesis. Biochem J. 416, 1-14

Trinkle-Mulcahy, L., Boulon, S., Lam, Y. W., Urcia, R., Boisvert, F. M., Vandermoere, F., Morrice, N. A., Swift, S., Rothbauer, U., Leonhardt, H. and Lamond, A. (2008) Identifying specific protein interaction partners using quantitative mass spectrometry and bead proteomes. J Cell Biol. 183, 223-239

Vazquez-Martin, C., Rouse, J. and Cohen, P. T. W. (2008) Characterization of the role of a trimeric protein phosphatase complex in recovery from cisplatin-induced versus noncrosslinking DNA damage. FEBS J. 275, 4211-4221

Villa, F., Deak, M., Alessi, D. R. and van Aalten, D. M. (2008) Structure of the OSR1 kinase, a hypertension drug target. Proteins. 73, 1082-1087

Windheim, M., Peggie, M. and Cohen, P. (2008) Two different classes of E2 ubiquitin-conjugating enzymes are required for the mono-ubiquitination of proteins and elongation by polyubiquitin chains with a specific topology. Biochem J. 409, 723-729

Windheim, M., Stafford, M., Peggie, M. and Cohen, P. (2008) Interleukin-1 (IL-1) induces the Lys63-linked polyubiquitination of IL-1 receptor-associated kinase 1 to facilitate NEMO binding and the activation of IkappaBalpha kinase. Mol Cell Biol. 28, 1783-1791

Yang, H. T., Cohen, P. and Rousseau, S. (2008) IL-1beta-stimulated activation of ERK1/2 and p38alpha MAPK mediates the transcriptional up-regulation of IL-6, IL-8 and GRO-alpha in HeLa cells. Cell Signal. 20, 375-380

Ying, Q. L., Wray, J., Nichols, J., Batlle-Morera, L., Doble, B., Woodgett, J., Cohen, P. and Smith, A. (2008) The ground state of embryonic stem cell self-renewal. Nature. 453, 519-523