University of Dundee

MRC Protein Phosphorylation and Ubiquitylation Unit 2014

Alessi, D. R., Zhang, J., Khanna, A., Hochdorfer, T., Shang, Y. and Kahle, K. T. (2014) The WNK-SPAK/OSR1 pathway: Master regulator of cation-chloride cotransporters. Sci Signal. 7, re3
doi. 10.1126/scisignal.2005365,

Bago, R., Malik, N., Munson, M. J., Prescott, A. R., Davies, P., Sommer, E., Shpiro, N., Ward, R., Cross, D., Ganley, I. G. and Alessi, D. R. (2014) Characterization of VPS34-IN1, a selective inhibitor of Vps34, reveals that the phosphatidylinositol 3-phosphate-binding SGK3 protein kinase is a downstream target of class III phosphoinositide 3-kinase. Biochem. J. 463, 413-427
doi. 10.1042/BJ20140889,
Pubmed. 4209782

Banerjee, S., Buhrlage, S. J., Huang, H. T., Deng, X., Zhou, W., Wang, J., Traynor, R., Prescott, A. R., Alessi, D. R. and Gray, N. S. (2014) Characterization of WZ4003 and HTH-01-015 as selective inhibitors of the LKB1-tumour-suppressor-activated NUAK kinases. Biochem. J. 457, 215-225
doi. 10.1042/BJ20131152,
Pubmed. 3969223

Banerjee, S., Zagorska, A., Deak, M., Campbell, D. G., Prescott, A. R. and Alessi, D. R. (2014) Interplay between Polo kinase, LKB1-activated NUAK1 kinase, PP1betaMYPT1 phosphatase complex and the SCFbetaTrCP E3 ubiquitin ligase. Biochem. J. 461, 233-245
doi. 10.1042/BJ20140408,
Pubmed. 4109838

Baron, Y., Pedrioli, P. G., Tyagi, K., Johnson, C., Wood, N. T., Fountaine, D., Wightman, M. and Alexandru, G. (2014) VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase. BMC Biol. 12, 39
doi. 10.1186/1741-7007-12-39,
Pubmed. 4068158

Chen, Q., Quan, C., Xie, B., Chen, L., Zhou, S., Toth, R., Campbell, D. G., Lu, S., Shirakawa, R., Horiuchi, H., Li, C., Yang, Z., MacKintosh, C., Wang, H. Y. and Chen, S. (2014) GARNL1, a major RalGAP alpha subunit in skeletal muscle, regulates insulin-stimulated RalA activation and GLUT4 trafficking via interaction with 14-3-3 proteins. Cell. Signal. 26, 1636-1648
doi. 10.1016/j.cellsig.2014.04.012,

Clark, K. (2014) Protein kinase networks that limit TLR signalling. Biochem. Soc. Trans. 42, 11-24
doi. 10.1042/BST20130124,

Cohen, P. (2014) The TLR and IL-1 signalling network at a glance. J. Cell Sci. 127, 2383-2390
doi. 10.1242/jcs.149831,

Cohen, P. (2014) Immune diseases caused by mutations in kinases and components of the ubiquitin system. Nat. Immunol. 15, 521-529
doi. 10.1038/ni.2892,

Cowling, V. H., Turner, S. A. and Cole, M. D. (2014) Burkitt's lymphoma-associated c-Myc mutations converge on a dramatically altered target gene response and implicate Nol5a/Nop56 in oncogenesis. Oncogene. 33, 3519-3527
doi. 10.1038/onc.2013.338,
de Los Heros, P., Alessi, D. R., Gourlay, R., Campbell, D. G., Deak, M., Macartney, T. J., Kahle, K. T. and Zhang, J. (2014) The WNK-regulated SPAK/OSR1 kinases directly phosphorylate and inhibit the K+-Cl- co-transporters. Biochem. J. 458, 559-573
doi. 10.1042/BJ20131478,
Pubmed. 3940040

Gawden-Bone, C., West, M. A., Morrison, V. L., Edgar, A. J., McMillan, S. J., Dill, B. D., Trost, M., Prescott, A., Fagerholm, S. C. and Watts, C. (2014) A crucial role for beta2 integrins in podosome formation, dynamics and Toll-like-receptor-signaled disassembly in dendritic cells. J. Cell Sci. 127, 4213-4224
doi. 10.1242/jcs.151167,
Pubmed. 4179490

Gonatopoulos-Pournatzis, T. and Cowling, V. H. (2014) RAM function is dependent on Kapbeta2-mediated nuclear entry. Biochem. J. 457, 473-484
doi. 10.1042/BJ20131359,
Pubmed. 3898117

Gonatopoulos-Pournatzis, T. and Cowling, V. H. (2014) Cap-binding complex (CBC). Biochem. J. 457, 231-242
doi. 10.1042/BJ20131214,
Pubmed. 3901397

Hamilton, J. J., Marlow, V. L., Owen, R. A., Costa Mde, A., Guo, M., Buchanan, G., Chandra, G., Trost, M., Coulthurst, S. J., Palmer, T., Stanley-Wall, N. R. and Sargent, F. (2014) A holin and an endopeptidase are essential for chitinolytic protein secretion in Serratia marcescens. J. Cell Biol. 207, 615-626
doi. 10.1083/jcb.201404127,
Pubmed. 4259817

Herhaus, L., Al-Salihi, M. A., Dingwell, K. S., Cummins, T. D., Wasmus, L., Vogt, J., Ewan, R., Bruce, D., Macartney, T., Weidlich, S., Smith, J. C. and Sapkota, G. P. (2014) USP15 targets ALK3/BMPR1A for deubiquitylation to enhance bone morphogenetic protein signalling. Open Biol. 4, 140065
doi. 10.1098/rsob.140065,
Pubmed. 4042855

Herhaus, L. and Sapkota, G. P. (2014) The emerging roles of deubiquitylating enzymes (DUBs) in the TGFbeta and BMP pathways. Cell. Signal. 26, 2186-2192
doi. 10.1016/j.cellsig.2014.06.012,

Hodson, C., Purkiss, A., Miles, J. A. and Walden, H. (2014) Structure of the human FANCL RING-Ube2T complex reveals determinants of cognate E3-E2 selection. Structure. 22, 337-344
doi. 10.1016/j.str.2013.12.004,
Pubmed. 3979106

Houde, V. P., Ritorto, M. S., Gourlay, R., Varghese, J., Davies, P., Shpiro, N., Sakamoto, K. and Alessi, D. R. (2014) Investigation of LKB1 Ser431 phosphorylation and Cys433 farnesylation using mouse knockin analysis reveals an unexpected role of prenylation in regulating AMPK activity. Biochem. J. 458, 41-56
doi. 10.1042/BJ20131324,
Pubmed. 3898322

Hunter, R. W., Foretz, M., Bultot, L., Fullerton, M. D., Deak, M., Ross, F. A., Hawley, S. A., Shpiro, N., Viollet, B., Barron, D., Kemp, B. E., Steinberg, G. R., Hardie, D. G. and Sakamoto, K. (2014) Mechanism of Action of Compound-13: An alpha1-Selective Small Molecule Activator of AMPK. Chem. Biol. 21, 866-879
doi. 10.1016/j.chembiol.2014.05.014,
Pubmed. 4104029

Iovino, M., Pfisterer, U., Holton, J. L., Lashley, T., Swingler, R. J., Calo, L., Treacy, R., Revesz, T., Parmar, M., Goedert, M., Muqit, M. M. and Spillantini, M. G. (2014) The novel MAPT mutation K298E: mechanisms of mutant tau toxicity, brain pathology and tau expression in induced fibroblast-derived neurons. Acta Neuropathol. 127, 283-295
doi. 10.1007/s00401-013-1219-1,
Pubmed. 3895181

Kazlauskaite, A., Kelly, V., Johnson, C., Baillie, C., Hastie, C. J., Peggie, M., Macartney, T., Woodroof, H. I., Alessi, D. R., Pedrioli, P. G. and Muqit, M. M. (2014) Phosphorylation of Parkin at Serine65 is essential for activation: elaboration of a Miro1 substrate-based assay of Parkin E3 ligase activity. Open Biol. 4, 130213
doi. 10.1098/rsob.130213,
Pubmed. 3971407

Kazlauskaite, A., Kondapalli, C., Gourlay, R., Campbell, D. G., Ritorto, M. S., Hofmann, K., Alessi, D. R., Knebel, A., Trost, M. and Muqit, M. M. (2014) Parkin is activated by PINK1-dependent phosphorylation of ubiquitin at Ser65. Biochem. J. 460, 127-139
doi. 10.1042/BJ20140334,
Pubmed. 4000136

Kim, M., Hunter, R. W., Garcia-Menendez, L., Gong, G., Yang, Y. Y., Kolwicz, S. C., Jr., Xu, J., Sakamoto, K., Wang, W. and Tian, R. (2014) Mutation in the gamma2-subunit of AMP-activated protein kinase stimulates cardiomyocyte proliferation and hypertrophy independent of glycogen storage. Circ. Res. 114, 966-975
doi. 10.1161/CIRCRESAHA.114.302364,
Pubmed. 3971100

Lachaud, C., Castor, D., Hain, K., Munoz, I., Wilson, J., MacArtney, T. J., Schindler, D. and Rouse, J. (2014) Distinct functional roles for the two SLX4 ubiquitin-binding UBZ domains mutated in Fanconi anemia. J. Cell Sci. 127, 2811-2817
doi. 10.1242/jcs.146167,
Pubmed. 4075355

Lin, K. M., Hu, W., Troutman, T. D., Jennings, M., Brewer, T., Li, X., Nanda, S., Cohen, P., Thomas, J. A. and Pasare, C. (2014) IRAK-1 bypasses priming and directly links TLRs to rapid NLRP3 inflammasome activation. Proc. Natl. Acad. Sci. U. S. A. 111, 775-780
doi. 10.1073/pnas.1320294111,
Pubmed. 3896167

Lopez-Pelaez, M., Lamont, D. J., Peggie, M., Shpiro, N., Gray, N. S. and Cohen, P. (2014) Protein kinase IKKbeta-catalyzed phosphorylation of IRF5 at Ser462 induces its dimerization and nuclear translocation in myeloid cells. Proc. Natl. Acad. Sci. U. S. A. 111, 17432-17437
doi. 10.1073/pnas.1418399111,
Pubmed. 4267347

Mackay, C., Carroll, E., Ibrahim, A. F., Garg, A., Inman, G. J., Hay, R. T. and Alpi, A. F. (2014) E3 Ubiquitin Ligase HOIP Attenuates Apoptotic Cell Death Induced by Cisplatin. Cancer Res. 74, 1-12  
doi. 10.1158/0008-5472.CAN-13-2131,
Pubmed. 3990471

Maric, M., Maculins, T., De Piccoli, G. and Labib, K. (2014) Cdc48 and a ubiquitin ligase drive disassembly of the CMG helicase at the end of DNA replication. Science. 346, 1253596
doi. 10.1126/science.1253596,
Pubmed. 4300516

Munoz, I. M., Szyniarowski, P., Toth, R., Rouse, J. and Lachaud, C. (2014) Improved Genome Editing in Human Cell Lines Using the CRISPR Method. PLoS One. 9, e109752
doi. 10.1371/journal.pone.0109752,
Pubmed. 4193831

Nair, N., Castor, D., Macartney, T. and Rouse, J. (2014) Identification and characterization of MUS81 point mutations that abolish interaction with the SLX4 scaffold protein. DNA Repair (Amst). 24, 131-137
doi. 10.1016/j.dnarep.2014.08.004,
Pubmed. 4251979

Naqvi, S., Martin, K. J. and Arthur, J. S. (2014) CREB phosphorylation at Ser133 regulates transcription via distinct mechanisms downstream of cAMP and MAPK signalling. Biochem. J. 458, 469-479
doi. 10.1042/BJ20131115,

Navarro, M. N., Feijoo-Carnero, C., Arandilla, A. G., Trost, M. and Cantrell, D. A. (2014) Protein kinase D2 is a digital amplifier of T cell receptor-stimulated diacylglycerol signaling in naive CD8+ T cells. Sci Signal. 7, ra99
doi. 10.1126/scisignal.2005477,
Patel, K., Foretz, M., Marion, A., Campbell, D. G., Gourlay, R., Boudaba, N., Tournier, E., Titchenell, P., Peggie, M., Deak, M., Wan, M., Kaestner, K. H., Goransson, O., Viollet, B., Gray, N. S., Birnbaum, M. J., Sutherland, C. and Sakamoto, K. (2014) The LKB1-salt-inducible kinase pathway functions as a key gluconeogenic suppressor in the liver. Nat Commun. 5, 4535
doi. 10.1038/ncomms5535,
Pubmed. 4143937

Pennell, S., Declais, A. C., Li, J., Haire, L. F., Berg, W., Saldanha, J. W., Taylor, I. A., Rouse, J., Lilley, D. M. and Smerdon, S. J. (2014) FAN1 Activity on Asymmetric Repair Intermediates Is Mediated by an Atypical Monomeric Virus-type Replication-Repair Nuclease Domain. Cell Rep. 8, 84-93
doi. 10.1016/j.celrep.2014.06.001,
Pubmed. 4103454

Pereira, E., Youssef, A., El-Ghozzi, A., Avignant, D., Bain, J., Prudhomme, M., Anizon, F. and Moreau, P. (2014) Synthesis of dipyrrolo[3,4-a:3,4-c]carbazoles: new kinase inhibitors. Tetrahedron Lett. 55, 834–837

Poncet, N., Mitchell, F. E., Ibrahim, A. F., McGuire, V. A., English, G., Arthur, J. S., Shi, Y. B. and Taylor, P. M. (2014) The catalytic subunit of the system L1 amino acid transporter (slc7a5) facilitates nutrient signalling in mouse skeletal muscle. PLoS One. 9, e89547
doi. 10.1371/journal.pone.0089547,
Pubmed. 3935884

Ritorto, M. S., Ewan, R., Perez-Oliva, A. B., Knebel, A., Buhrlage, S. J., Wightman, M., Kelly, S. M., Wood, N. T., Virdee, S., Gray, N. S., Morrice, N. A., Alessi, D. R. and Trost, M. (2014) Screening of DUB activity and specificity by MALDI-TOF mass spectrometry. Nat Commun. 5, 4763
doi. 10.1038/ncomms5763,
Pubmed. 4147353

Schonhuber, N., Seidler, B., Schuck, K., Veltkamp, C., Schachtler, C., Zukowska, M., Eser, S., Feyerabend, T. B., Paul, M. C., Eser, P., Klein, S., Lowy, A. M., Banerjee, R., Yang, F., Lee, C. L., Moding, E. J., Kirsch, D. G., Scheideler, A., Alessi, D. R., Varela, I., Bradley, A., Kind, A., Schnieke, A. E., Rodewald, H. R., Rad, R., Schmid, R. M., Schneider, G. and Saur, D. (2014) A next-generation dual-recombinase system for time- and host-specific targeting of pancreatic cancer. Nat. Med. 20, 1340-1347
doi. 10.1038/nm.3646,
Schumacher, F. R., Sorrell, F. J., Alessi, D. R., Bullock, A. N. and Kurz, T. (2014) Structural and biochemical characterization of the KLHL3-WNK kinase interaction important in blood pressure regulation. Biochem. J. 460, 237-246
doi. 10.1042/BJ20140153,
Pubmed. 4019986

Simon, A. C., Zhou, J. C., Perera, R. L., van Deursen, F., Evrin, C., Ivanova, M. E., Kilkenny, M. L., Renault, L., Kjaer, S., Matak-Vinkovic, D., Labib, K., Costa, A. and Pellegrini, L. (2014) A Ctf4 trimer couples the CMG helicase to DNA polymerase alpha in the eukaryotic replisome. Nature. 510, 293-297
doi. 10.1038/nature13234,
Pubmed. 4059944

Spratt, D. E., Walden, H. and Shaw, G. S. (2014) RBR E3 ubiquitin ligases: new structures, new insights, new questions. Biochem. J. 458, 421-437
doi. 10.1042/BJ20140006,
Pubmed. 3940038

Tape, C. J., Worboys, J. D., Sinclair, J., Gourlay, R., Vogt, J., McMahon, K. M., Trost, M., Lauffenburger, D. A., Lamont, D. J. and Jorgensen, C. (2014) Reproducible automated phosphopeptide enrichment using magnetic TiO2 and Ti-IMAC. Anal. Chem. 86, 10296-10302
doi. 10.1021/ac5025842,
Pubmed. 4206527

Timmermans, A. D., Balteau, M., Gelinas, R., Renguet, E., Ginion, A., de Meester, C., Sakamoto, K., Balligand, J. L., Bontemps, F., Vanoverschelde, J. L., Horman, S., Beauloye, C. and Bertrand, L. (2014) A-769662 potentiates the effect of other AMP-activated protein kinase activators on cardiac glucose uptake. Am. J. Physiol. Heart Circ. Physiol. 306, H1619-1630
doi. 10.1152/ajpheart.00965.2013,

Tinti, M., Dissanayake, K., Synowsky, S., Albergante, L. and Mackintosh, C. (2014) Identification of 2R-ohnologue gene families displaying the same mutation-load skew in multiple cancers. Open Biol. 4, 140029
doi. 10.1098/rsob.140029,
Pubmed. 4042849

Tinti, M., Madeira, F., Murugesan, G., Hoxhaj, G., Toth, R. and Mackintosh, C. (2014) ANIA: ANnotation and Integrated Analysis of the 14-3-3 interactome. Database (Oxford). 2014, bat085
doi. 10.1093/database/bat085,
Pubmed. 3914767

van der Wijst, J., Blanchard, M. G., Woodroof, H. I., Macartney, T. J., Gourlay, R., Hoenderop, J. G., Bindels, R. J. and Alessi, D. R. (2014) Kinase and channel activity of TRPM6 are co-ordinated by a dimerization motif and pocket interaction. Biochem. J. 460, 165-175
doi. 10.1042/BJ20131639,
Pubmed. 4019984

Vogt, J., Dingwell, K. S., Herhaus, L., Gourlay, R., Macartney, T., Campbell, D., Smith, J. C. and Sapkota, G. P. (2014) Protein associated with SMAD1 (PAWS1/FAM83G) is a substrate for type I bone morphogenetic protein receptors and modulates bone morphogenetic protein signalling. Open Biol. 4, 130210
doi. 10.1098/rsob.130210,
Pubmed. 3938053

Walden, H. and Deans, A. J. (2014) The fanconi anemia DNA repair pathway: structural and functional insights into a complex disorder. Annu Rev Biophys. 43, 257-278
doi. 10.1146/annurev-biophys-051013-022737,

Zeqiraj, E., Tang, X., Hunter, R. W., Garcia-Rocha, M., Judd, A., Deak, M., von Wilamowitz-Moellendorff, A., Kurinov, I., Guinovart, J. J., Tyers, M., Sakamoto, K. and Sicheri, F. (2014) Structural basis for the recruitment of glycogen synthase by glycogenin. Proc. Natl. Acad. Sci. U. S. A. 111, E2831-2840
doi. 10.1073/pnas.1402926111,
Pubmed. 4104858

Zhang, J., Clark, K., Lawrence, T., Peggie, M. W. and Cohen, P. (2014) An unexpected twist to the activation of IKKbeta: TAK1 primes IKKbeta for activation by autophosphorylation. Biochem. J. 461, 531-537
doi. 10.1042/BJ20140444,
Pubmed. 4206954