University of Dundee

MRC Protein Phosphorylation Unit 2010

Akue-Gedu, R., Nauton, L., Thery, V., Bain, J., Cohen, P., Anizon, F. and Moreau, P. (2010) Synthesis, Pim kinase inhibitory potencies and in vitro antiproliferative activities of diversely substituted pyrrolo[2,3-a]carbazoles. Bioorg Med Chem, 18, 6865-6873.

Blomster, H. A., Imanishi, S. Y., Siimes, J., Kastu, J., Morrice, N. A., Eriksson, J. E. and Sistonen, L. (2010) In vivo identification of sumoylation sites by a signature tag and cysteine-targeted affinity purification. J Biol Chem, 285, 19324-19329.

Bouskila, M., Hunter, R. W., Ibrahim, A. F., Delattre, L., Peggie, M., van Diepen, J. A., Voshol, P. J., Jensen, J. and Sakamoto, K. (2010) Allosteric regulation of glycogen synthase controls glycogen synthesis in muscle. Cell Metab, 12, 456-466.

Cohen, P. (2010) Guidelines for the effective use of chemical inhibitors of protein function to understand their roles in cell regulation. Biochem J, 425, 53-54.

Cohen, P. and Tcherpakov, M. (2010) Will the ubiquitin system furnish as many drug targets as protein kinases? Cell, 143, 686-693.

Cohen, P. T. W. (2010) Sorting the protein phosphatases: okadaic acid led the way. The Biochemist, 32, 22-27. (Read online)

Darragh, J., Ananieva, O., Courtney, A., Elcombe, S. and Arthur, J. S. (2010) MSK1 regulates the transcription of IL-1ra in response to TLR activation in macrophages. Biochem J, 425, 595-602.

Dunne, A., Carpenter, S., Brikos, C., Gray, P., Strelow, A., Wesche, H., Morrice, N. and O'Neill, L. A. (2010) IRAK1 and IRAK4 promote phosphorylation, ubiquitination, and degradation of MyD88 adaptor-like (Mal). J Biol Chem, 285, 18276-18282.

Duro, E., Lundin, C., Ask, K., Sanchez-Pulido, L., MacArtney, T. J., Toth, R., Ponting, C. P., Groth, A., Helleday, T. and Rouse, J. (2010) Identification of the MMS22L-TONSL complex that promotes homologous recombination. Mol Cell, 40, 632-644.

Dzamko, N., Deak, M., Hentati, F., Reith, A. D., Prescott, A. R., Alessi, D. R. and Nichols, R. J. (2010) Inhibition of LRRK2 kinase activity leads to dephosphorylation of Ser(910)/Ser(935), disruption of 14-3-3 binding and altered cytoplasmic localization. Biochem J, 430, 405-413.

Finlay, D. K., Kelly, A. P., Clarke, R., Sinclair, L. V., Deak, M., Alessi, D. R. and Cantrell, D. A. (2010) Temporal differences in the dependency on phosphoinositide-dependent kinase 1 distinguish the development of invariant Valpha14 NKT cells and conventional T cells. J Immunol, 185, 5973-5982.

Foretz, M., Hebrard, S., Leclerc, J., Zarrinpashneh, E., Soty, M., Mithieux, G., Sakamoto, K., Andreelli, F. and Viollet, B. (2010) Metformin inhibits hepatic gluconeogenesis in mice independently of the LKB1/AMPK pathway via a decrease in hepatic energy state. J Clin Invest, 120, 2355-2369.

Greenblatt, M. B., Shim, J. H., Zou, W., Sitara, D., Schweitzer, M., Hu, D., Lotinun, S., Sano, Y., Baron, R., Park, J. M., Arthur, S., Xie, M., Schneider, M. D., Zhai, B., Gygi, S., Davis, R. and Glimcher, L. H. (2010) The p38 MAPK pathway is essential for skeletogenesis and bone homeostasis in mice. J Clin Invest, 120, 2457-2473.

Inesta-Vaquera, F. A., Campbell, D. G., Arthur, J. S. and Cuenda, A. (2010) ERK5 pathway regulates the phosphorylation of tumour suppressor hDlg during mitosis. Biochem Biophys Res Commun, 399, 84-90.

Inesta-Vaquera, F. A., Campbell, D. G., Tournier, C., Gomez, N., Lizcano, J. M. and Cuenda, A. (2010) Alternative ERK5 regulation by phosphorylation during the cell cycle. Cell Signal, 22, 1829-1837.

Johnson, C., Crowther, S., Stafford, M. J., Campbell, D. G., Toth, R. and Mackintosh, C. (2010) Bioinformatic and experimental survey of 14-3-3-binding sites. Biochem J, 427, 69-78.

Lai, Y. C., Zarrinpashneh, E. and Jensen, J. (2010) Additive effect of contraction and insulin on glucose uptake and glycogen synthase in muscle with different glycogen contents. J Appl Physiol, 108, 1106-1115.

Lewis, A. E., Sommer, L., Arntzen, M. O., Strahm, Y., Morrice, N. A., Divecha, N. and D'Santos, C. S. (2010) Identification of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins by neomycin extraction. Mol Cell Proteomics.

Mackay, C., Declais, A. C., Lundin, C., Agostinho, A., Deans, A. J., Macartney, T. J., Hofmann, K., Gartner, A., West, S. C., Helleday, T., Lilley, D. M. and Rouse, J. (2010) Identification of KIAA1018/FAN1, a DNA Repair Nuclease Recruited to DNA Damage by Monoubiquitinated FANCD2. Cell, 142, 65-76.

Martin, D. M., Nett, I. R., Vandermoere, F., Barber, J. D., Morrice, N. A. and Ferguson, M. A. (2010) Prophossi: automating expert validation of phosphopeptide-spectrum matches from tandem mass spectrometry. Bioinformatics, 26, 2153-2159.

Martin, D. M., Nett, I. R., Vandermoere, F., Barber, J. D., Morrice, N. A. and Ferguson, M. A. (2010) Prophossi: automating expert validation of phosphopeptide-spectrum matches from tandem mass spectrometry. Bioinformatics, 26, 2153-2159.

Middelbeek, J., Clark, K., Venselaar, H., Huynen, M. A. and van Leeuwen, F. N. (2010) The alpha-kinase family: an exceptional branch on the protein kinase tree. Cell Mol Life Sci, 67, 875-890.

Monk, C. E., Hutvagner, G. and Arthur, J. S. (2010) Regulation of miRNA transcription in macrophages in response to Candida albicans. PLoS One, 5, e13669.

Najafov, A. and Alessi, D. R. (2010) Uncoupling the Warburg effect from cancer. Proc Natl Acad Sci U S A, 107, 19135-19136.

Najafov, A., Sommer, E. M., Axten, J. M., Deyoung, M. P. and Alessi, D. R. (2010) Characterization of GSK2334470, a novel and highly specific inhibitor of PDK1. Biochem J, 433, 357-369.

Nichols, R. J., Dzamko, N., Morrice, N. A., Campbell, D. G., Deak, M., Ordureau, A., Macartney, T., Tong, Y., Shen, J., Prescott, A. R. and Alessi, D. R. (2010) 14-3-3 binding to LRRK2 is disrupted by multiple Parkinson's disease-associated mutations and regulates cytoplasmic localization. Biochem J, 430, 393-404.

Pearce, L. R., Alton, G. R., Richter, D. T., Kath, J. C., Lingardo, L., Chapman, J., Hwang, C. and Alessi, D. R. (2010) Characterization of PF-4708671, a novel and highly specific inhibitor of p70 ribosomal S6 kinase (S6K1). Biochem J, 431, 245-255.

Pearce, L. R., Komander, D. and Alessi, D. R. (2010) The nuts and bolts of AGC protein kinases. Nat Rev Mol Cell Biol, 11, 9-22.

Peirce, M. J., Brook, M., Morrice, N., Snelgrove, R., Begum, S., Lanfrancotti, A., Notley, C., Hussell, T., Cope, A. P. and Wait, R. (2010) Themis2/ICB1 is a signaling scaffold that selectively regulates macrophage Toll-like receptor signaling and cytokine production. PLoS One, 5, e11465.

Peirce, M. J., Brook, M., Morrice, N., Snelgrove, R., Begum, S., Lanfrancotti, A., Notley, C., Hussell, T., Cope, A. P. and Wait, R. (2010) Themis2/ICB1 is a signaling scaffold that selectively regulates macrophage Toll-like receptor signaling and cytokine production. PLoS One, 5, e11465.

Pozo-Guisado, E., Campbell, D. G., Deak, M., Alvarez-Barrientos, A., Morrice, N. A., Alvarez, I. S., Alessi, D. R. and Martin-Romero, F. J. (2010) Phosphorylation of STIM1 at ERK1/2 target sites modulates store-operated calcium entry. J Cell Sci, 123, 3084-3093.

Pozuelo-Rubio, M., Leslie, N. R., Murphy, J. and Mackintosh, C. (2010) Mechanism of activation of PKB/Akt by the protein phosphatase inhibitor Calyculin A. Cell Biochem Biophys, 58, 147-156.

Rafiqi, F. H., Zuber, A. M., Glover, M., Richardson, C., Fleming, S., Jovanovic, S., Jovanovic, A., O'Shaughnessy, K. M. and Alessi, D. R. (2010) Role of the WNK-activated SPAK kinase in regulating blood pressure. EMBO Mol Med, 2, 63-75.

Reiche, J., Theilig, F., Rafiqi, F. H., Carlo, A. S., Militz, D., Mutig, K., Todiras, M., Christensen, E. I., Ellison, D. H., Bader, M., Nykjaer, A., Bachmann, S., Alessi, D. and Willnow, T. E. (2010) SORLA/SORL1 functionally interacts with SPAK to control renal activation of Na(+)-K(+)-Cl(-) cotransporter 2. Mol Cell Biol, 30, 3027-3037.

Remenyi, J., Hunter, C. J., Cole, C., Ando, H., Impey, S., Monk, C. E., Martin, K. J., Barton, G. J., Hutvagner, G. and Arthur, J. S. (2010) Regulation of the miR-212/132 locus by MSK1 and CREB in response to neurotrophins. Biochem J, 428, 281-291.

Sabio, G., Cerezo-Guisado, M. I., Del Reino, P., Inesta-Vaquera, F. A., Rousseau, S., Arthur, J. S., Campbell, D. G., Centeno, F. and Cuenda, A. (2010) p38gamma regulates interaction of nuclear PSF and RNA with the tumour-suppressor hDlg in response to osmotic shock. J Cell Sci, 123, 2596-2604.

Sanchez Canedo, C., Demeulder, B., Ginion, A., Bayascas, J. R., Balligand, J. L., Alessi, D. R., Vanoverschelde, J. L., Beauloye, C., Hue, L. and Bertrand, L. (2010) Activation of the cardiac mTOR/p70(S6K) pathway by leucine requires PDK1 and correlates with PRAS40 phosphorylation. Am J Physiol Endocrinol Metab, 298, E761-769.

Sicard, P., Clark, J. E., Jacquet, S., Mohammadi, S., Arthur, J. S., O'Keefe, S. J. and Marber, M. S. (2010) The activation of p38 alpha, and not p38 beta, mitogen-activated protein kinase is required for ischemic preconditioning. J Mol Cell Cardiol, 48, 1324-1328.

Soutar, M. P., Kim, W. Y., Williamson, R., Peggie, M., Hastie, C. J., McLauchlan, H., Snider, W. D., Gordon-Weeks, P. R. and Sutherland, C. (2010) Evidence that glycogen synthase kinase-3 isoforms have distinct substrate preference in the brain. J Neurochem, 115, 974-983.

Toh, G. W., Sugawara, N., Dong, J., Toth, R., Lee, S. E., Haber, J. E. and Rouse, J. (2010) Mec1/Tel1-dependent phosphorylation of Slx4 stimulates Rad1-Rad10-dependent cleavage of non-homologous DNA tails. DNA Repair (Amst), 9, 718-726.

Wang, L., Gordon, R. A., Huynh, L., Su, X., Park Min, K. H., Han, J., Arthur, J. S., Kalliolias, G. D. and Ivashkiv, L. B. (2010) Indirect inhibition of Toll-like receptor and type I interferon responses by ITAM-coupled receptors and integrins. Immunity, 32, 518-530.

Wullschleger, S., Sakamoto, K., Johnstone, L., Duce, S., Fleming, S. and Alessi, D. R. (2010) How moderate changes in Akt T-loop phosphorylation impact on tumorigenesis and insulin resistance. Dis Model Mech, 4, 95-103.

Yan, L., Mieulet, V., Burgess, D., Findlay, G. M., Sully, K., Procter, J., Goris, J., Janssens, V., Morrice, N. A. and Lamb, R. F. (2010) PP2A T61 epsilon is an inhibitor of MAP4K3 in nutrient signaling to mTOR. Mol Cell, 37, 633-642.

Zagorska, A., Deak, M., Campbell, D. G., Banerjee, S., Hirano, M., Aizawa, S., Prescott, A. R. and Alessi, D. R. (2010) New roles for the LKB1-NUAK pathway in controlling myosin phosphatase complexes and cell adhesion. Sci Signal, 3, ra25.