University of Dundee

Molecular Physiology 2010

Alhopuro, P., Bjorklund, M., Sammalkorpi, H., Turunen, M., Tuupanen, S., Bistrom, M., Niittymaki, I., Lehtonen, H. J., Kivioja, T., Launonen, V., Saharinen, J., Nousiainen, K., Hautaniemi, S., Nuorva, K., Mecklin, J. P., Jarvinen, H., Orntoft, T., Arango, D., Lehtonen, R., Karhu, A., Taipale, J. and Aaltonen, L. A. (2010) Mutations in the circadian gene CLOCK in colorectal cancer. Mol Cancer Res, 8, 952-960.

Davidson, L., Maccario, H., Perera, N. M., Yang, X., Spinelli, L., Tibarewal, P., Glancy, B., Gray, A., Weijer, C. J., Downes, C. P. and Leslie, N. R. (2010) Suppression of cellular proliferation and invasion by the concerted lipid and protein phosphatase activities of PTEN. Oncogene, 29, 687-697.

Ellis, I. R., Jones, S. J., Lindsay, Y., Ohe, G., Schor, A. M., Schor, S. L. and Leslie, N. R. (2010) Migration Stimulating Factor (MSF) promotes fibroblast migration by inhibiting AKT. Cell Signal, 22, 1655-1659.

Fogarty, S. and Hardie, D. G. (2010) Development of protein kinase activators: AMPK as a target in metabolic disorders and cancer. Biochim. Biophys. Acta, 1804, 581-591.

Fogarty, S., Hawley, S. A., Green, K. A., Saner, N., Mustard, K. J. and Hardie, D. G. (2010) Calmodulin-dependent protein kinase kinase-beta activates AMPK without forming a stable complex - synergistic effects of Ca2+ and AMP. Biochem. J., 426, 109-118.

Foukas, L. C., Berenjeno, I. M., Gray, A., Khwaja, A. and Vanhaesebroeck, B. (2010) Activity of any class IA PI3K isoform can sustain cell proliferation and survival. Proc Natl Acad Sci U S A, 107, 11381-11386.

Hale, B. G., Kerry, P. S., Jackson, D., Precious, B. L., Gray, A., Killip, M. J., Randall, R. E. and Russell, R. J. (2010) Structural insights into phosphoinositide 3-kinase activation by the influenza A virus NS1 protein. Proc Natl Acad Sci U S A, 107, 1954-1959.

Hardie, D. G. (2010) Sensing of energy and nutrients by the AMP-activated protein kinase (AMPK). Am. J. Clin. Nutr., in press.

Hardie, D. G. (2010) Energy-sensing by the AMP-activated protein kinase and its effects on muscle metabolism. Proc. Nutr. Soc., in press.

Hardie, D. G. (2010) Transcription. Targeting the core of transcription. Science, 329, 1158-1159.

Hardie, D. G. (2010) Hot stuff: thyroid hormones and AMPK. Cell Res., 20, 1282-1284.

Hawley, S. A., Ross, F. A., Chevtzoff, C., Green, K. A., Evans, A., Fogarty, S., Towler, M. C., Brown, L. J., Ogunbayo, O. A., Evans, A. M. and Hardie, D. G. (2010) Use of cells expressing gamma subunit variants to Identify diverse mechanisms of AMPK activation. Cell Metab., 11, 554-565.

Kiilerich, K., Adser, H., Jakobsen, A. H., Pedersen, P. A., Hardie, D. G., Wojtaszewski, J. F. and Pilegaard, H. (2010) PGC-1alpha increases PDH content but does not change acute PDH regulation in mouse skeletal muscle. Am. J. Physiol. Regul. Integr. Comp. Physiol.

Leslie, N. R., Spinelli, L., Tibarewal, P., Zilidis, G., Weerasinghe, N., Lim, J. C., Maccario, H. and Downes, C. P. (2010) Indirect mechanisms of carcinogenesis via downregulation of PTEN function. Adv Enzyme Regul, 50, 112-118.

Lipina, C., Kendall, H., McPherron, A. C., Taylor, P. M. and Hundal, H. S. Mechanisms involved in the enhancement of mammalian target of rapamycin signalling and hypertrophy in skeletal muscle of myostatin-deficient mice. FEBS Lett, 584, 2403-2408.

Lipina, C., Kendall, H., McPherron, A. C., Taylor, P. M. and Hundal, H. S. (2010) Mechanisms involved in the enhancement of mammalian target of rapamycin signalling and hypertrophy in skeletal muscle of myostatin-deficient mice. FEBS Lett, 584, 2403-2408.

Lipina, C., Stretton, C., Hastings, S., Hundal, J. S., Mackie, K., Irving, A. J. and Hundal, H. S. Regulation of MAP kinase-directed mitogenic and protein kinase B-mediated signaling by cannabinoid receptor type 1 in skeletal muscle cells. Diabetes, 59, 375-385.

Logie, L., Ruiz-Alcaraz, A. J., Schofield, C. J., Hundal, H. S., Feuerstein, G. Z., Brady, J. D., Crowther, D., Tommasi, A. M., Grierson, C. E., Shepherd, B., Morris, A. D., Hansen, M. K., Pearson, E. and Sutherland, C. Generation, validation and humanisation of a novel insulin resistant cell model. Biochem Pharmacol, 80, 1042-1049.

Loubiere, L. S., Vasilopoulou, E., Bulmer, J. N., Taylor, P. M., Stieger, B., Verrey, F., McCabe, C. J., Franklyn, J. A., Kilby, M. D. and Chan, S. Y. (2010) Expression of thyroid hormone transporters in the human placenta and changes associated with intrauterine growth restriction. Placenta, 31, 295-304.

Maccario, H., Perera, N. M., Gray, A., Downes, C. P. and Leslie, N. R. (2010) Ubiquitination of PTEN (phosphatase and tensin homolog) inhibits phosphatase activity and is enhanced by membrane targeting and hyperosmotic stress. J Biol Chem, 285, 12620-12628.

Mankouri, J., Tedbury, P. R., Gretton, S., Hughes, M. E., Griffin, S. D., Dallas, M. L., Green, K. A., Hardie, D. G., Peers, C. and Harris, M. (2010) Enhanced hepatitis C virus genome replication and lipid accumulation mediated by inhibition of AMP-activated protein kinase. Proc. Natl. Acad. Sci. USA, 107, 11549-11554.

McDonald, A., Fogarty, S., Leclerc, I., Hill, E. V., Hardie, D. G. and Rutter, G. A. (2010) Cell-wide analysis of secretory granule dynamics in three dimensions in living pancreatic beta-cells: evidence against a role for AMPK-dependent phosphorylation of KLC1 at Ser(517)/Ser(520) in glucose-stimulated insulin granule movement. Biochem Soc Trans, 38, 205-208.

Mitchell, F. E., Roy, L. A. and Taylor, P. M. (2010) Iodothyronine Interactions with the System L1 Amino Acid Exchanger in 3T3-L1 Adipocytes. Journal of Thyroid Research, 2010, 726098.

Moreno, D., Towler, M. C., Hardie, D. G., Knecht, E. and Sanz, P. (2010) The laforin-malin complex, involved in Lafora disease, promotes the incorporation of K63-linked ubiquitin chains into AMP-activated protein kinase beta subunits. Mol. Biol. Cell, 21, 2578-2588.

Moult, P. R., Cross, A., Santos, S. D., Carvalho, A. L., Lindsay, Y., Connolly, C. N., Irving, A. J., Leslie, N. R. and Harvey, J. (2010) Leptin regulates AMPA receptor trafficking via PTEN inhibition. J Neurosci, 30, 4088-4101.

Paterson, M. J., Dunsmore, C. J., Hurteaux, R., Maltman, B. A., Cotton, G. J. and Gray, A. (2010) A fluorescence lifetime-based assay for serine and threonine kinases that is suitable for high-throughput screening. Anal Biochem, 402, 54-64.

Pozuelo-Rubio, M., Leslie, N. R., Murphy, J. and Mackintosh, C. (2010) Mechanism of Activation of PKB/Akt by the Protein Phosphatase Inhibitor Calyculin A. Cell Biochem Biophys.

Ritchie, J. W. and Taylor, P. M. (2010) Tryptophan and iodothyronine transport interactions in HepG2 human hepatoma cells. Amino Acids, 38, 1361-1367.

Stretton, C., Evans, A. and Hundal, H. S. Cellular depletion of atypical PKC{lambda} is associated with enhanced insulin sensitivity and glucose uptake in L6 rat skeletal muscle cells. Am J Physiol Endocrinol Metab, 299, E402-412.

Timmers, S., de Vogel-van den Bosch, J., Towler, M. C., Schaart, G., Moonen-Kornips, E., Mensink, R. P., Hesselink, M. K., Hardie, D. G. and Schrauwen, P. (2010) Prevention of high-fat diet-induced muscular lipid accumulation in rats by alpha lipoic acid is not mediated by AMPK activation. J. Lipid Res., 51, 352-359.

Zhou, K., Bellenguez, C., Spencer, C. C., Bennett, A. J., Coleman, R. L., Tavendale, R., Hawley, S. A., Donnelly, L. A., Schofield, C., Groves, C. J., Burch, L., Carr, F., Strange, A., Freeman, C., Blackwell, J. M., Bramon, E., Brown, M. A., Casas, J. P., Corvin, A., Craddock, N., Deloukas, P., Dronov, S., Duncanson, A., Edkins, S., Gray, E., Hunt, S., Jankowski, J., Langford, C., Markus, H. S., Mathew, C. G., Plomin, R., Rautanen, A., Sawcer, S. J., Samani, N. J., Trembath, R., Viswanathan, A. C., Wood, N. W., Harries, L. W., Hattersley, A. T., Doney, A. S., Colhoun, H., Morris, A. D., Sutherland, C., Hardie, D. G., Peltonen, L., McCarthy, M. I., Holman, R. R., Palmer, C. N., Donnelly, P. and Pearson, E. R. (2010) Common variants near ATM are associated with glycemic response to metformin in type 2 diabetes. Nat. Genet.