University of Dundee

CSC - Functions & Applications of a Novel Stem Cell Signalling Pathway

The Findlay lab employs cutting-edge technologies to unravel Embryonic Stem (ES) cell signalling networks (Williams et al, Cell Rep 2016, Fernandez-Alonso et al, EMBO Rep 2017; Bustos et al, Cell Rep 2018), culminating in our recent discovery of the ERK5 pathway as an exciting new regulator of ES cell pluripotency. In order to uncover functions of ERK5 in ES cells, this project will deploy global quantitative phosphoproteomic, proteomic and transcriptomic (RNA-SEQ) profiling. Novel ERK5 substrates and transcriptional networks will be characterised using biochemical and ES cell biology approaches. A further aim is to investigate functions of ERK5 in human patient-derived iPS cell maintenance and lineage-specific differentiation to neurons, cardiomyocytes etc. This research will be undertaken in the MRC Protein Phosphorylation and Ubiquitylation Unit (MRC-PPU), one of the world’s leading centres studying the role of cell signalling in health and disease. Students will have access to state-of-the-art tools to dissect signalling networks, and opportunity for interaction with three major Pharmaceutical companies that support the MRC-PPU via the Division of Signal Transduction Therapy. The Findlay lab is also affiliated with the Division of Cell and Developmental Biology, which comprises world experts in stem cell research.

Recent work from the lab can be found in the following references: 

  1. Bustos, F.*, Segarra-Fas, A.*, Chaugule, V.K., Brandenburg, L., Branigan, E., Toth, R., Macartney, T., Knebel, A., Hay, R.T., Walden, H. and Findlay, G.M. (2018) Cell Rep. 23(6): 1599-1611
  2. Fernandez-Alonso, R., Davidson, L., Hukelmann, J., Zengerle, M., Prescott, A.R., Lamond, A., Ciulli, A., Sapkota, G.P. and Findlay, G.M. (2017) EMBO Rep. 18(7):1108-1122
  3. Williams, C.A.C., Fernandez-Alonso, R., Wang, J., Gray, N.S. and Findlay, G.M. (2016) Cell Rep. 16(6):1820-8