University of Dundee

Dr Edgar Huitema

Mechanisms of virulence acquisition during Phytophthora-host associations
Position: 
Senior Lecturer
Affiliation: 
Address: 
University of Dundee at JHI, Errol Road, Invergowrie, Dundee
Full Telephone: 
+44 (0) 1382 568921, int ext
Email: 

Research

Investigation of Phytophthora-host associations. Left panel: N. benthamiana leaf inoculated with P. capsici zoospores. P. capsici infection features an eary biotrophic stage, tissue darkening and necrosis, tissue maceration and sporulation. Right panel: Ectopic expression of pathogen genes in tobacco cells induces cell death. Secreted pathogen proteins target and modify host cellular components. Ectopic expression of such proteins often leads to phenotypic manifestations such as cell death, reflecting functional roles towards virulence.

For more information about the Huitema Lab, please visit our Group webpage.

Publications

2013

  1. Stam, R., Howden, A. J. M., Delgado-Cerezo, M., Amaro, T. M. M. M., Motion, G. B., Pham, J. and Huitema, E. Characterisation of cell death inducing Phytophthora capsici CRN effectors suggests diverse activities in the host nucleus. Frontiers in Plant-Microbe Interaction (in press)
  2. Stam, R., Jupe, J., Howden, A. J. M., Morris, J. A., Boevink, P. C., Hedley, P. E. & Huitema, E. (2013). Identification and Characterisation CRN Effectors in Phytophthora capsici Shows Modularity and Functional Diversity. In : PLoS ONE. 8, 3, 13 p., e59517
  3. Jupe, J., Stam, R., Howden, A. J. M., Morris, J. A., Zhang, R., Hedley, P. E. & Huitema, E. (2013). Phytophthora capsici-tomato interaction features dramatic shifts in gene expression associated with a hemi-biotrophic lifestyle In : Genome Biology. 14, 6, R63

2012

  1. Howden, A.J. & Huitema, E. (2012). Effector-triggered post-translational modifications and their role in suppression of plant immunity. Front Plant Sci. 2012;3:160. Epub 2012 Jul 16. PubMed PMID: 22811685
  2. Lamour, K. H  et al., 2012. Genome Sequencing and Mapping Reveal Loss of Heterozygosity as a Mechanism for Rapid Adaptation in the Vegetable Pathogen Phytophthora capsici. Molecular Plant - Microbe Interactions. 25 1350-1360.
  3. Bozkurt, T.O., Schornack, S., Win, J., Shindo, T., Ilyas, M., Oliva, R., Cano, L.M., Jones, A.M.E., Huitema, E., van der Hoorn, R.A.L. & Kamoun, S. (2011) Phytophthora Infestans effector AVRblb2 prevents secretion of a plant immune protease at the haustorial interface. Proc Natl Acad Sci USA 108, 20832-20837

2011

  1. Bozkurt, T.O., Schornack, S., Win, J., Shindo, T., Ilyas, M., Oliva, R., Cano, L.M., Jones, A.M.E., Huitema, E., van der Hoorn, R.A.L. & Kamoun, S. (2011) Phytophthora Infestans effector AVRblb2 prevents secretion of a plant immune protease at the haustorial interface. Proc Natl Acad Sci USA 108, 20832-20837
  2. Lamour K.H., Stam R., Jupe J. and Huitema E. (2011). The Oomycete Broad Host range pathogen Phytophthora capsici. Molecular Plant Pathology, DOI: 10.1111/j.1364-3703.2011.00754.x.

2010

  1. Levesque AC, Brouwer H, Cano LM, Hamilton JP, Holt C, Huitema E, Raffaele S, Robideau GP, Thines M, Win J, et al. (2010). Genome sequence of the necrotrophic plant pathogen, Pythium ultimum, reveals original pathogenicity mechanisms and effector repertoire. Genome Biology 11:R73.
  2. Schornack S., Van Damme, M., Bozkurt, T.O., Cano, L.M., Smoker, M., Thines, M., Gaulin, E., Kamoun, S. and Huitema, E. (2010). Ancient class of translocated oomycete effectors targets the host nucleus. Proc Natl Acad Sci USA (Epub Sept 16, 2010, doi: 10.1073/pnas. 1008491107
  3. Oliva R., Win J., Raffaele S., Boutemy L., Bozkurt T.O., Chaparro-Garcia A., Segretin M.E., Stam R., Schornack S., Cano L.M., van Damme M., Huitema E., Thines M., Banfield M.J. and Kamoun S. 2010. Recent developments in effector biology of filamentous plant pathogens. Cell Microbiol. 2010 Mar 31.
  4. Green S., Studholme, D.J., Laue B.J., Dorati F., Lovell H., Arnold D., Cottrell J.E., Bridgett S., Blaxter M., Huitema, E., Thwaites R., Sharp P.M., Jackson R.W. and Kamoun S. 2010. Comparative Genome Analysis Provides Insights into the Evolution and Adaptation of Pseudomonas syringae pv. aesculi on Aesculus hippocastanum. PLoS ONE 5(4): e10224. doi:10.1371
  5. Hughes HV, Huitema E, Pritchard S, Keiler KC, Brun YV and Viollier PH. 2010 Protein localization and dynamics within a bacterial organelle. Proc Natl Acad Sci U S A. 107(12):5599-604. Epub 2010 Mar 8

2009

  1. Schornack S., Fuchs R., Huitema E. , Rothbauer U., Lipka V., and Kamoun S. 2009. Protein mislocalization in plant cells using a GFP-binding chromobody. The Plant Journal Epub, July
  2. Haas, B.J., Kamoun, S., Zody, M.C., Jiang, R.H.Y., Handsaker, R.E., Cano, L.M., Grabherr, M., Kodira, C.D., Raffaele, S., Torto-Alalibo, T., Bozkurt, T.O., Ah-Fong, A.M.V., Alvarado, L., Anderson, V.L., Armstrong, M.R., Avrova, A., Baxter, L., Beynon, J., Boevink, P.C., Bollmann, S.R., Bos, J.I.B., Bulone, V., Cai, G., Cakir, C., Carrington, J.C., Chawner, M., Conti, L., Costanzo, S., Ewan, R., Fahlgren, N., Fischbach, M.A., Fugelstad, J., Gilroy, E.M., Gnerre, S., Green, P.J., Grenville-Briggs, L.J., Griffith, J., Grunwald, N.J., Horn, K., Horner, N.R., Hu, C.-H., Huitema, E., Jeong, D.-H., Jones, A.M.E., Jones, J.D.G., Jones, R.W., Karlsson, E.K., Kunjeti, S.G., Lamour, K., Liu, Z., Ma, L., MacLean, D., Chibucos, M.C., McDonald, H., McWalters, J., Meijer, H.J.G., Morgan, W., Morris, P.F., Munro, C.A., O'Neill, K., Ospina-Giraldo, M., Pinzon, A., Pritchard, L., Ramsahoye, B., Ren, Q., Restrepo, S., Roy, S., Sadanandom, A., Savidor, A., Schornack, S., Schwartz, D.C., Schumann, U.D., Schwessinger, B., Seyer, L., Sharpe, T., Silvar, C., Song, J., Studholme, D.J., Sykes, S., Thines, M., van de Vondervoort, P.J.I., Phuntumart, V., Wawra, S., Weide, R., Win, J., Young, C., Zhou, S., Fry, W., Meyers, B.C., van West, P., Ristaino, J., Govers, F., Birch, P.R.J., Whisson, S.C., Judelson, H.S., and Nusbaum, C. 2009. Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans. Nature 461:393-398..
  3. Schornack S., Huitema E., Cano L. M., Bozkurt T. O., Oliva R., van Damme M., Schwizer S., Rafaelle S., Chaparro-Garcia A., Farrer R., Segretin M. A., Bos J. I. B., Haas B. J., Zody M. C., Nusbaum C., Win J., Thines M. and Kamoun S. 2009. Ten things to know about oomycete effectors. Molecular Plant Pathology, 10(6): 795-803
  4. Huitema, E., Smoker M. and Kamoun, S. 2009. A straightforward protocol for electro-transformation of Phytophthora capsici zoospores. Chapter in: Methods in Molecular Biology, The Plant Immune Response In press
  5. Van Damme, M., Schornack, S., Cano L. M., Huitema, E. and Kamoun, S. 2009 Interactions Between Phytophthora Infestans And Solanum. Chapter in: Oomycete Genetics and Genomics: Diversity, Interactions and Research Tools In press.

2007

  1. Huitema, E. and Viollier, P.H. 2007. Break on through to the other side: outer membrane penetration of the nascent flagellum by a stop-polymerization mechanism. Genes & Development 21: 2253-2257
  2. Kanneganti, T., Huitema, E. and Kamoun, S. 2007. In planta expression of oomycete and fungal genes. Book chapter in: Methods in Molecular Biology 354: 35-43

2006

  1. Bos, J.I.B., Kanneganti, T. D., Young, C., Cakir, C., Huitema, E., Win, J., Armstrong, M.R., Birch, P.R.J. and Kamoun, S. 2006. The C-terminal half of Phytophthora infestans RXLR effector AVR3a is sufficient to trigger R3a-mediated hypersensitivity and suppress INF1-induced cell death in Nicotiana benthamiana. Plant Journal 48:165-176.
  2. Huitema E. and Viollier, P.H. 2006. A Myxobacterial S-motility protein Dances with poles. Trends in Microbiology 14(6): 247-248
  3. Huitema, E., Pritchard, S., Matteson, D., Kumar Radhakrishnan, S. and Viollier, P.H. 2006. Bacterial Birth Scar Proteins Mark Future Flagellum Assembly Site. Cell 124: 1025-1037.
  4. Kanneganti, T. D.*, Huitema E.*, Cakir, C. and Kamoun S. 2006. Synergistic interactions of the plant cell death pathways induced by Phytophthora infestans Nep1-like protein PiNPP1.1 and NF1 elicitin. Molecular Plant-Microbe Interactions 19(8): 854-863. (* TD and EH contributed equally)

2005

  1. Huitema E., Vleeshouwers V.G.A.A., Cakir C., Kamoun S., and Govers F. 2005. Differences in intensity and specificity of hypersensitive response induction in Nicotiana by INF1, INF2A and INF2B of Phytophthora infestans. Molecular Plant-Microbe Interactions 18(3): 183-193.
  2. Randall T. A., Dwyer R. A., Huitema E., Beyer K., Cvitanich C., Kelkar H., Ah Fong A., Gates K., Roberts S.,Yatzkan E., Gaffney T., Law M., Testa A.,Torto T., Zhang M., Zheng L., Mueller E., Windass J., Binder A., Birch P. R. J. , Gisi U., Govers F., Gow N., Mauch F., van West P., Waugh M., Yu J., Boller T., Kamoun S., Lam S. T., and Judelson H. S. 2005. Large-scale gene discovery in the oomycete Phytophtora infestans reveals likely components of phytopathogenicity shared with true fungi. Molecular Plant-Microbe Interactions 18(3): 229-243.

2004

  1. Tian, M., Huitema, E., da Cunha, L., Torto-Alalibo, T., and Kamoun, S. 2004. A Kazal-like extracellular serine protease inhibitor from Phytophthora infestans targets the tomato pathogenesis-related protease P69B. Journal of Biological Chemistry 279: 26370-26377.
  2. Huitema, E., Bos, J. I. B., Tian, M., Win, J., Waugh, M. E. and Kamoun, S. 2004. Linking sequence to phenotype in Phytophthora-plant interactions. Trends in Microbiology, 12:193-200.

2003

  1. Huitema, E., Vleeshouwers, V. G. A. A., Francis, D. M., and Kamoun, S. 2003. Active defence responses associated with non-host resistance of Arabidopsis thaliana to the oomycete pathogen Phytophthora infestans. Molecular Plant Pathology 4: 487-500.
  2. Torto, T., Li, S., Styer, A., Huitema, E., Testa, A., Gow, N.A.R., van West, P., and Kamoun, S. 2003. EST mining and functional expression assays identify extracellular effector proteins from Phytophthora. Genome Research 13:1675-1685.
  3. Qutob, D.*, Huitema, E., Gijzen, M., and Kamoun, S. 2003. Variation in structure and activity among elicitins from Phytophthora sojae. Molecular Plant Pathology 4: 119-124 .
  4. Kamoun, S., Hamada, W., and Huitema, E. 2003. Agrosuppression: a bioassay for the hypersensitive response suited to high-throughput screening. Molecular Plant-Microbe Interactions 16: 7-13.
  5. Huitema, E., Torto, T.A., Styer, A., and Kamoun, S. 2003. Combined ESTs from plant-microbe interactions: Using GC counting to determine the species of origin. Chapter in: Functional genomics: Methods and Protocols 79-83.

2002

  1. Torto, T.A., Testa, A., Styer, A., Morgan, W.R., Kinney, D., Huitema, E., Hamada, W., Dong, S., and Kamoun, S. 2002. Applying the sequence-to-phenotype functional genomics paradigm to Phytophthora. In: Biology of Plant-Microbe Interactions 3: 331-335.
  2. Kamoun, S., Dong, S., Hamada, W., Huitema, E., Kinney, D., Morgan, W.R., Styer, A., Testa, A., and Torto, T. 2002. From sequence to phenotype: Functional genomics of Phytophthora. Canadian Journal of Plant Pathology 24: 6-9

2000

  1. Kamoun, S., Dong, S., Huitema, E., Torto, G.A., van West, P., Vleeshouwers, V.G.A.A. and Govers, F. 2000. Dissection of nonhost resistance of Nicotiana to Phytophthora infestans. In: Proceedings of the 9th International Congress for Molecular Plant-Microbe Interactions 180-185.