Tom Owen-Hughes Group


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1. SWI/SNF Sliding

swislide file icon

These files summarise our SWI/SNF sliding experiements as presented at several meetings during spring-summer 1999 and published in our recent Nature paper.

view pdf file

swislide.pdf (207kb)

A 3 page PDF file adapted from posters shown at four meetings and conferences.

  • You will need the free Acrobat Reader (v3 or above) to view this file.

2. Animation of trans displacement

dinucleosome formation

This animation demonstrate a hypothesis for how trans displacemnent of histone octamers on DNA could occur. It accounts for the dinucleosomal intermediate previously observed by several groups,as diagrammed above.

view animation

transdpl.gif

Clicking on this .gif file will display the animated gif file in a separate window.

  • You can view this directly in your web browser window without extra software.

3. Quicktime movies of sliding

These movies were made to clarify the two most probable mechanisms by which nucleosome sliding can occur. We are working on versions to illustrate the way in which the motions could be generated.

view movie

twist.mov
bulging.mov bulging.avi
twist.avi

Clicking on these .mov files should cause a new browser window to launch and, after some buffering time, the Quicktime or AVI movie to play within it. The AVI versions are higher resolution versions.

  • You will need the free Quicktime Player version 3 or above (preferably v4) correctly installed as a helper with your web browser.
download compressed movie

worming.zip (54kb)
worming.mov.sit.hqx (77kb)
bulging.zip (4.2 Mb)
bulging.mov.sit.hqx (5.9Mb)

These are compressed versions of the same files as above for independent download to view on your local computer. Alternatively, save one of the uncompressed links above directly to your local drive.

  • You will need the free Quicktime Player version 3 or above (preferably v4) installed.
  • For decompression we recommend StuffIt Expander (Mac) for .sit.hqx archives, and WinZip (Windows) for Zip archives.

Bulge transiting nucleosome