Research

 

Publications

2017

KDM2 family members are regulated by HIF-1 in hypoxia. 2017. Batie M, Druker J, D'Ignazio L, and Rocha S. Cells. 6, 8. PMID: 28304334.

2016

Potent and selective chemical probe of hypoxic signalling downstream of HIF-alpha hydroxylation via VHL. 2016. Frost F, Galdeano C, Soares P, Gadd MS, Grzes KM, Ellis L, Epemolu O, Shimamura S, Bantcheff M, Grandi P, Read KD, Cantrell DA, Rocha S, and Ciulli A. Nat. Comms. PMID: 27811928.

Intricate Macrophage-Colorectal Cancer Cell Communication in Response to Radiation. 2016. Pinto AT, Pinto ML, Velho S, Pinto MT, Cardoso AP, Figueira R, Monteiro A, Marques M, Seruca R, Barbosa MA, Mareel M, Oliveira MJ, and Rocha S. Plos One. PMID: 27513864.

Enhanced SnoMen Vectors facilitate establishment of GFP-HIF-1alpha protein replacement human cell lines. 2016. Ono M, Yamada K, Bensaddek D, Afzal V, Biddlestone J, Ortmann B, Mudie S, Boivin V, Scott MS, Rocha S, and Lamond AI. Plos One. PMID: 27128805.

The chromatin remodeller SNF2H and SNF2L reposition nucleosomes adjacent to CTCF and other transcription factors. 2016. Wiechens N, Singh V, Gkikopoulos T, Schofield P, Rocha S, and Owen-Hughes T. Plos Genetics. PMID: 27019336.

Hypoxia induced NF-kappaB. 2016. D'Ignazio L, and Rocha S. Cells. PMID: 27005664

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd Edition). 2016. Klionsky DJ, et al. Autophagy. PMID: 26799652.

Ionizing radiation modulates human macrophages towards a pro-inflammatory phenotype preserving their pro-invasive and pro-angiogenic capacities. 2016. Pinto AT, Pinto ML, Cradoso AP, Pinto MT, Maia AF, Castro P, Figueira R, Monteiro A, Marques M, Mareel M, Dos Santos SG, Seruca R, Barbosa MA, Rocha S, Oliveira MJ. Sci. Rep. PMID: 26735768.

CDK dependent phosphorylation of PHD1 on Serine 130 alters its substrate preference in cells. 2016. Ortmann B, Bensaddek D, Carvalhal S, Moser S, Mudie S, Griffis ER, Swedlow JR, Lamond AI, and Rocha S. J. Cell Sci. PMID: 26644182.

2015

NF-kappaB and HIF crosstalk in immune responses. 2015. D'Ignazio L, Bandarra D, and Rocha. FEBS J. PMID: 26513405.

Cezanne regulates E2F1-dependent HIF-2alpha expression. 2015. Moniz S, Bandarra D, Biddlestone J, Campbell KJ, Komander D, Bremm A, and Rocha S. J. Cell Sci. PMID: 26148512.

HIF-1alpha, a novel piece in the NF-kappaB puzzle. Bandarra D, and Rocha S. 2015. Inflammation and Cell Signalling. 2(1). DOI.

Dose dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia. Sun Y., George J., and Rocha S. 2015. Plos One. PMID: 25830774.

The role of Hypoxia in Inflammatory disease. Biddlestone J, Bandarra D, and Rocha S. 2015. Int. J. Mol. Med.PMID: 25625467.

HIF-1alpha restricts NF-kappaB-dependent gene expression to control innate immunity signals. Bandarra D, Biddlestone J, Mudie S, Muller HA, and Rocha S. 2015. Dis Model Mech. PMID: 25510503.

2014

PITX1, a specificity determinant in the HIF-1alpha-mediated transcriptional response to hypoxia. Mudie S, Bandarra D, Batie M, Biddlestone J, Moniz S, Ortmann B, Shmakova A, and Rocha S. 2014. Cell Cycle. PMID: 25558831.

Cezanne (OTUD7B) regulates HIF-1alpha homeostasis in a proteasome independent manner. Bremm A, Moniz S, Mader J, Rocha S, and Komander D. 2014. EMBO rep. PMID: 25355043.

Chromatin and oxygen sensing in the context of JmjC histone demethylases. Shmakova A, Batie M, Druker J, and Rocha S. 2014. Sep 15; 462(3): 385-395. Biochem. J. PMID: 25145438

Hypoxia activates IKK-NF-kappaB and the immune system in Drosophila melanogaster. Bandarra D, Biddlestone J, Mudie S, Muller HA, and Rocha S. 2014. Biosci. Rep. PMID: 24993778

siRNA screening to identify Ubiquitin and Ibiquitin-like system regulators of biological pathways in cultured mammalian cells. Bett JS, Ibraim AF, Garg AK, Rocha S, and Hay RT. 2014 May 24; 87: J. Vis Exp. PMID: 24893647

Cell Cycle Progression in Response to Low Oxygen levels. Ortmann B., Druker J, and Rocha S. 2014 May 25 Cell Mol Life Sci. PMID: 24858415

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia. Biddlestone J, Druker J, Shmakova A, Ferguson G, Swedlow JR, and Rocha S. 2014 May 13; 87: J. Vis Exp. PMID: 24895009

Grow2: The HIF system, energy homeostasis and the cell cycle. Moniz S, Biddlestone J, and Rocha S. 2014 May 29 (5): 589-600; Histology and Histopathology. PMID: 24407868

2013

A tale of two transcription factors: NF-kappaB and HIF crosstalk. Bandarra D, and Rocha S. 2013 Jun 1; 1(1):6. OA Molecular and Cell Biology.

PHD1 links Cell-Cycle progression to oxygen sensing through hydroxylation of the centrosomal protein Cep192. Moser S, Bensaddek D, Ortmann B, Maure JF, Mudie S, Blow JJ, Lamond AI, Swedlow JR, and Rocha S. 2013 Aug 26; 26(4): 381-392. Dev. Cell. PMID: 23932902

FMN2 is a novel regulator of the cyclin-dependent kinase inhibitor p21. Yamada K, Ono M, Bensaddek D, Lamond AI, and Rocha S. 2013 Aug 1; 12(15): 2348-2354. Cell Cycle. PMID: 23839046

The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability. Bett JS, Ibrahim AF, Garg AK, Kelly V, Pedrioli P, Rocha S, Hay RT. 2013 Apr 15; 451(2):185-194. Biochemical Journal. PMID: 23398456

Identification and functional characterization of FMN2, a regulator of the cyclin dependent kinase inhibitor p21. Yamada K, Ono M, Perkins ND, Rocha S, Lamond AI. 2013 Mar 7; 49(5):922-933. Molecular Cell. PMID: 23375502

HIF-indenpendent role of prolyl hydroxylases in the cellular response to amino acids. Duran RV, MacKenzie ED, Boulahbek H, Frezza C, Heiserich L, Tardito S., Bussolati O, Rocha S, Hall MN, and Gottlieb E. 2013 Sep 19; 32(38): 4549-4556. Oncogene. PMID: 23085753

TFR1 interacts with the IKK complex and is involved in IKK-NF-kappaB signalling. Kenneth NS, Mudie S, Naron S, and Rocha S. 2013 Jan 1; 449(1): 275-284. Biochemical Journal. PMID:23016877

2012

Proteomic screen reveals Fbw7 as a modulator of the NF-kappaB pathway. Arabi A, Ullah K, Branca RMM, Johansson J, Bandarra D, Haneklaus M, Fu J, Aries I, Nilsson P, Den Boer ML, Pokrovskala K, Grander D, Xiao G, Rocha S, Lehtio J, Sangfelt O. 2012; 3:976. Nat. Comms. PMID: 2286469

FAM60A is a cell cycle-fluctuating regulator of the SIN3-HDAC1 histonde deacetylase complex. Munoz IM, Macartney T, Sanchez-Pulido L, Ponting CP, Rocha S, Rouse J. 2012 Sep 21; 287(39): 32346-32353. J. Biol. Chem. PMID:22865885

Chromatin as an oxygen sensor and an active player in the hypoxia response. Melvin A, Rocha S. Cell. Signal. 2012 Jan; 24(1): 35-43. PMID: 21924352

2011

The chromatin remodeler ISWI regulates the cellular response to hypoxia, role of FIH. Melvin A, Mudie S, Rocha S. Mol. Biol. Cell. 2011 Nov; 22(21): 4171-4181. PMID: 21900490

NF-kappaB controls energy homeostasis and metabolic adaptation by upregulating mitochondria respiration. Mauro C, Leow SC, Anso E, Rocha S, Thotakura AK, Tornatore L, Moretti M, De Smaele E, Beg AA, Tergaonkar V, Chandel NS, Franzoso G. Nat. Cell. Biol. 2011 Aug; 13(10):1272-1279. PMID: 21968997

The role of RelA(p65) Threonine 505 phosphorylation in the regulation of cell growth, survival and migration. Msaki A, Sanchez AM, Koh LF, Barre B, Rocha S, Perkins ND, Johnson RF. Mol. Biol. Cell. 2011 Sep; 22(17):3032-3040. PMID: 21737676

Antagonistic crosstalk between APC and HIF-1alpha. Nathke I, Rocha S. Cell Cycle. 2011 May; 10(10):1545-1547. PMID: 21502812

HIF-1alpha depletion results in SP1-mediated cell cycle disruption and alters the cellular response to chemotherapeutic drugs. Culver C, Melvin A, Mudie S, Rocha S. Cell Cycle. 2011 Apr; 10(8):1249-1260. PMID: 21412054

Further insights into the mechanism of hypoxia-induced NFkappaB. Melvin A, Mudie S, Rocha S. Cell Cycle. 2011 Mar; 10(6):879-882. PMID: 21325892

Evolutionary conserved regulation of HIF-1beta by NF-kappaB. van Uden P, Kenneth NS, Webster R, Muller HA, Mudie S, Rocha S. Plos Genetics. 2011 Jan; 7(1):e1001285.PMID: 21298084

2010

Adenomatous polyposis coli and hypoxia-inducible-factor-1alpha have an antagonistic connection. Newton IP, Kenneth NS, Appleton PL, Nathke I, Rocha S. Mol. Biol. Cell. 2010 Nov; 21(21):3630-3638. PMID: 20844082

Mechanism of Hypoxia Induced NF-kappaB. Culver C, Sundqvist A, Mudie S, Melvin A, Xirodimas D, Rocha S. Mol Cell Biol. Oct; 2010 Oct; 30(20):4901-4921. PMID: 20696840

IKK and NF-kappaB-mediated regulation of Claspin impacts on ATR checkpoint function. Kenneth NS, Mudie S, Rocha S. EMBO J. 2010 Sep 1;29(17):2966-2978. PMID: 20657549

2009

SWI/SNF regulates the cellular response to hypoxia. Kenneth NS, Mudie S, van Uden P, Rocha S. J Biol Chem. 2009 Feb 13;284(7):4123-4131. PMID: 19097995

2008

Kenneth NS, Rocha S. Regulation of gene expression by hypoxia. Biochem J. 2008 Aug 15;414(1):19-29. PMID: 18651837

van Uden P, Kenneth NS, Rocha S. Regulation of hypoxia-inducible factor-1alpha by NF-kappaB. Biochem J. 2008 Jun 15;412(3):477-484. PMID: 18393939

2007

Rocha S. Gene regulation under low oxygen: holding your breath for transcription. Trends Biochem Sci. 2007 Aug;32(8):389-397. Epub 2007 Jul 10. Review. PMID: 17624786

Roche KC, Rocha S, Bracken CP, Perkins ND. Regulation of ATR-dependent pathways by the FHA domain containing protein SNIP1. Oncogene. 2007 Jul 5;26(31):4523-4530. Epub 2007 Jan 29. PMID: 17260016

2006

Schumm K, Rocha S, Caamano J, Perkins ND. Regulation of p53 tumour suppressor target gene expression by the p52 NF-kappaB subunit. EMBO J. 2006 Oct 18;25(20):4820-4832. Epub 2006 Sep 21. PMID: 16990795

Campbell KJ, Witty JM, Rocha S, Perkins ND. Cisplatin mimics ARF tumor suppressor regulation of RelA (p65) nuclear factor-kappaB transactivation. Cancer Res. 2006 Jan 15;66(2):929-935. PMID: 16424027

2005

Rocha S, Perkins ND. ARF the integrator: linking NF-kappaB, p53 and checkpoint kinases. Cell Cycle. 2005 Jun;4(6):756-759. Epub 2005 Jun 13. PMID: 15970704

Rocha S, Garrett MD, Campbell KJ, Schumm K, Perkins ND. Regulation of NF-kappaB and p53 through activation of ATR and Chk1 by the ARF tumour suppressor. EMBO J. 2005 Mar 23;24(6):1157-1169. Epub 2005 Mar 10. PMID: 15775976

2004

Campbell KJ, Rocha S, Perkins ND. Active repression of antiapoptotic gene expression by RelA(p65) NF-kappa B. Mol Cell. 2004 Mar 26;13(6):853-865. PMID: 15053878

2003

Rocha S, Campbell KJ, Roche KC, Perkins ND. The p53-inhibitor pifithrin-alpha inhibits firefly luciferase activity in vivo and in vitro. BMC Mol Biol. 2003 Sep 11;4:9. PMID: 12967348

Rocha S, Campbell KJ, Perkins ND. p53- and Mdm2-independent repression of NF-kappa B transactivation by the ARF tumor suppressor. Mol Cell. 2003 Jul;12(1):15-25. PMID: 12887889

Rocha S, Martin AM, Meek DW, Perkins ND. p53 represses cyclin D1 transcription through down regulation of Bcl-3 and inducing increased association of the p52 NF-kappaB subunit with histone deacetylase 1. Mol Cell Biol. 2003 Jul;23(13):4713-4727. PMID: 12808109