Menu

Karim Labib's Lab

Home

Karim Labib

Research

Current Projects
Public Engagement

Publications

The Group

Present
Past

Where are we?

MRC PPU_School of Life Sciences
Dundee
Within 5 miles of Dundee
St. Andrews
Exploring Scotland

Contact

Publications (2001 onwards)

View all Karim's publications via PubMed

2020 Deegan, T.D., Mukherjee, P.P., Fujisawa, R., Polo Rivera, C., Labib, K.P.M. (2020) CMG disassembly is controlled by replication fork DNA, replisome components and a ubiquitin threshold. eLife, 9:e60371. doi: 10.7554/eLife.60371. 2019 Sonneville, R., Bhowmick, R. Hoffmann, S., Mailand, N., Hickson, I.D., Labib, K. (2019) TRAIP drives replisome disassembly and mitotic DNA repair synthesis at sites of incomplete DNA replication. eLife, 8:e48686. Doi: 10.7554/eLife.48686. Mukherjee, P.P. and Labib, K.P.M. (2019) In Vitro Reconstitution Defines the Minimal Requirements for Cdc48-dependent Disassembly of the CMG helicase in Budding Yeast. Cell Rep., 28, 2777-2783. Deegan, T.D., Baxter, J., Ortiz-Bazan, M.A., Yeeles, T.P., Labib, K.P.M. (2019) Pif1-family helicases support fork convergence during DNA replication termination in eukaryotes. Mol. Cell, 18, 231-244. Deng L, Wu RA, Sonneville R, Kochenova OV, Labib K, Pellman D, Walter JC. (2019) Mitotic CDK Promotes Replisome Disassembly, Fork Breakage, and Complex DNA Rearrangements. Mol Cell, 73, 915-929. 2018 Evrin C, Maman JD, Diamante A, Pellegrini L, Labib K. (2018) Histone H2A-H2B binding by Pol α in the eukaryotic replisome contributes to the maintenance of repressive chromatin. EMBO J., 37, pii: e99021. doi: 10.15252/embj.201899021 Gan, H., Serra-Cardona, A., Hua, X, Zhou, H., Labib, K., Yu, C., Zhang, Z. (2018) The Mcm2-Ctf4-Polα axis facilitates parental histone H3-H4 transfer to lagging strands. Mol. Cell, 72, 140-151. Hong Y, Velkova M, Silva N, Jagut M, Scheidt V, Labib K, Jantsch V, Gartner A. (2018) The conserved LEM-3/Ankle1 nuclease is involved in the combinatorial regulation of meiotic recombination repair and chromosome segregation in Caenorhabditis elegans. PLoS Genet. 14(6):e1007453. doi: 10.1371/journal.pgen.1007453. Hong, Y., Sonneville, R., Wang, B., Scheidt, V., Meier, B., Woglar, A., Demetriou, S., Labib, K., Jantsch, V., and Gartner, A. (2018) LEM-3 is a midbody-tethered nuclease that resolves chromatin bridges during late mitosis. Nat. Commun. 9, 728. doi: 10.1038/s41467-018-03135-w. 2017 Wu Y., Villa F., Maman J., Dobnikar L., Lau Y.H., Simon A.C., Labib K., Spring D.R. and Pellegrini L. (2017) Targeting the genome stability hub Ctf4 by stapled peptide design. Angew Chem Int Ed Engl. doi: 10.1002/anie.201705611. Sonneville R, Moreno SP, Knebel A, Johnson C, Hastie CJ, Gartner A, Gambus A, Labib K. (2017) CUL-2LRR-1 and UBXN-3 drive replisome disassembly during DNA replication termination and mitosis. Nat Cell Biol. 19, 468-479. Maric M, Mukherjee P, Tatham MH, Hay R, Labib K. (2017) Ufd1-Npl4 Recruit Cdc48 for Disassembly of Ubiquitylated CMG Helicase at the End of Chromosome Replication. Cell Rep. 18, 3033-3042. 2016 Villa, F., Simon, A. C., Ortiz-Bazan, M. A., Kilkenny, M. L., Wirthensohn, D., Wightman, M., Matak-Vinkovic, D., Pellegrini, L. and Labib, K. (2016) Ctf4 is a hub in the eukaryotic replisome that links multiple CIP-box proteins to the CMG helicase. Mol. Cell, 63, 1-12. Abdul Rehman, S. A., Kristariyanto, Y. A., Choi, S-Y., Nkosi, P. J., Weidlich, S., Labib, K., Hofmann, K. and Kulathu, Y. (2016) MINDY-1 Is a Member of an Evolutionarily Conserved and Structurally Distinct New Family of Deubiquitinating Enzymes. Mol. Cell, 63, 146-155. Bell, S. P. and Labib, K. (2016) Chromosome Duplication in Saccharomyces cerevisiae. Genetics, 203, 1027-1067.. Buser, R., Kellner, V., Melnik, A., Wilson-Zbinden, C., Schellhaas, R., Kastner, L., Piwko, W., Dees, M., Picotti, P., Maric, M., Labib, K., Luke, B. and Peter, M. (2016) The replisome-coupled E3 ubiquitin ligase Rtt101Mms22 counteracts Mrc1 function to tolerate genotoxic stress. PLOS Genetics, DOI:10.1371/journal.pgen.1005843. 2015 Maculins, T., Nkosi, P. J., Nishikawa, H. and Labib, K. (2015). Tethering of SCF to the Replisome Promotes Efficient Ubiquitylation and Disassembly of the CMG Helicase. Curr. Biol., 25, 2254-2259. Garcia-Rodriguez, L. J., De Piccoli, G., Marchesi, V., Jones, R. C., Edmondson, R. D. and Labib, K. (2015). A conserved Pol epsilon binding module in Ctf18-RFC is required for S-phase checkpoint activation downstream of Mec1. Nucleic Acids Res., 43, 8830-8838. Sonneville, R., Craig, G., Labib, K., Gartner, A. and Blow, J. J. (2015). Both Chromosome Decondensation and Condensation Are Dependent on DNA Replication in C. elegans Embryos. Cell Rep. 12, 405-417. 2014 Maric, M., Maculins, T., De Piccoli, G. and Labib, K. (2014). Cdc48 and a ubiquitin ligase drive disassembly of the CMG helicase at the end of DNA replication. Science, 346 (6208):1253596. Doi: 10.1126/science.1253596. Simon, A. C., Zhou, J. C., Perera, R. L., van Deursen, F., Evrin, C., Ivanova, M. E., Kilkenny, M. L., Renault, L., Kjaer, S., Matak-Vinkovic, D., Labib, K., Costa, A. and Pellegrini, L. (2014). A Ctf4 trimer couples the CMG helicase to DNA polymerase alpha in the eukaryotic replisome. Nature, 510, pp. 293-297. 2013 Foltman, M., Evrin, C., De Piccoli, G., Jones, R.C., Edmondson, R.D., Katou, Y., Nakato, R., Shirahige, K. and Labib, K. (2013) Eukaryotic replisome components cooperate to process histones during chromosome replication. Cell Reports, 3, 892-904. Sengupta, S., van Deursen, F., De Piccoli, G. and Labib, K. (2013) Dpb2 integrates the leading strand DNA polymerase into the eukaryotic replisome. Curr. Biol., 23, 543-552. Nkosi, P., Targosz, B.-S., Labib, K., Sanchez-Diaz, A. (2013) Hof1 and Rvs167 have redundant roles in actomyosin ring function during cytokinesis in budding yeast. PLOS ONE, 8, e57846. doi: 10.1371/journal.pone.0057846. 2012 De Piccoli, G., Katou, Y., Itoh, T., Nakato, R., Shirahige, K., and Labib, K. (2012) Replisome Stability at Defective DNA Replication Forks Is Independent of S Phase Checkpoint Kinases. Mol. Cell, 45, 696-704. Devrekanli, A., Foltman, M., Roncero, C., Sanchez-Diaz, A. and Labib, K. (2012) Inn1 and Cyk3 regulate chitin synthase during cytokinesis in budding yeasts. J. Cell Sci., 125, 5453-5466. Sanchez-Diaz, A., Nkosi, P., Murray, S. and Labib, K. (2012) The Mitotic Exit Network and Cdc14 phosphatase initiate cytokinesis by counteracting CDK phosphorylations and blocking polarised growth. EMBO J., 31, 3620-3634. Kilkenny, M.L., De Piccoli, G., Perera, R.L., Labib, K.* and Pellegrini, L.* (2012) A conserved motif in the C-terminal tail of DNA polymerase α tethers primase to the eukaryotic replisome. J. Biol. Chem., 287, 23740-23747. *co-corresponding authors van Deursen F, Sengupta S, De Piccoli G, Sanchez-Diaz A, Labib K. (2012) Mcm10 associates with the loaded DNA helicase at replication origins and defines a novel step in its activation. EMBO J., 31, 2195-2206. 2011 Labib, K. (2011) Building a double hexamer of DNA helicase at eukaryotic replication origins. EMBO J., 30, 4853-4855. Labib, K. and De Piccoli, G. (2011) Surviving chromosome replication: the many roles of the S-phase checkpoint pathway. Phil. Trans. Roy. Soc. B, 366, 3554-3561. 2010 Labib, K. (2010) How do Cdc7 and cyclin dependent kinases trigger the initiation of chromosome replication in eukaryotic cells? Genes Dev., 24, 1208-1219. 2009 Morohashi, H., Maculins, T., Labib, K. (2009) The amino terminal TPR domain of Dia2 connects SCFDia2 to the Replisome Progression Complex. Curr. Biol., 19, 1943-1949. Gambus, A., van Deursen, F., Polychronopoulos D, Foltman M, Jones RC, Edmondson RD, Calzada A, Labib K. (2009) A key role for Ctf4 in coupling the MCM2-7 helicase to DNA polymerase alpha within the eukaryotic replisome. EMBO J., 28, 2992-3004. 2008 Sanchez-Diaz A, Marchesi V, Murray S, Jones R, Pereira G, Edmondson R, Allen T, Labib K. (2008) Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast. Nat. Cell Biol., 10, 395-406. De la Cueva-Mendez, G. and Labib, K. (2008) New insights into the chromosome cycle. EMBO Rep., 9, 1177-81. Labib, K. (2008) Labib, K. (2008) Making connections at DNA replication forks: Mrc1 takes the lead. Mol. Cell, 32 ,166-8. 2007 Hodgson, B., Calzada, A. and Labib, K. (2007) Mrc1 and Tof1 regulate DNA replication forks in different ways during normal S phase. Mol. Biol. Cell (18), 3894-3902. Labib, K., and Gambus, A. (2007) A key role for the GINS complex at DNA replication forks. Trends Cell Biol. 17, 271-278. Labib, K. (2007) Highlights of the 23rd Genes and Cancer meeting University of Warwick 11-13 December 2006. Oncogene, 26, 2675-2677 Labib, K. and Hodgson, B. (2007) Replication fork barriers: pausing for a break or stalling for time? EMBO Rep., 8, 346-353. 2006 Kanemaki, M. and Labib, K. (2006) Distinct roles for Sld3 and GINS during establishment and progression of eukaryotic DNA replication forks. EMBO J., 25, 1753-1763. Gambus, A., Jones, R.C., Sanchez-Diaz, A., Kanemaki, M., van Deursen, F., Edmondson, R.D., and Labib, K. (2006) GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forks. Nat. Cell Biol., 8, 358-366. Schwob, E. and Labib, K. (2006) Regulating Initiation events in Yeasts. In DNA replication and human disease (ed. M.L. DePamphilis), pp. 295-312. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York . 2005 Calzada, A., Hodgson, B., Kanemaki, M., Bueno, A. and Labib, K. (2005). Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication fork. Genes Dev., 19, 1905-1919. 2004 A. Sanchez-Diaz, M. Kanemaki, V. Marchesi, K. Labib (2004). Rapid Depletion of Budding Yeast Proteins by Fusion to a Heat-Inducible Degron. Sci. STKE 2004, pl8. 2003 Kanemaki, M, Sanchez-Diaz, A., Gambus, A. and Labib, K. (2003) Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo. Nature, 423, 720-724. 2002 Diffley, J.F.X. and Labib, K. (2002) The Chromosome Replication Cycle. J.Cell Sci. 115, 869-872.

View all Karim's publications via PubMed

2020 Deegan, T.D., Mukherjee, P.P., Fujisawa, R., Polo Rivera, C., Labib, K.P.M. (2020) CMG disassembly is controlled by replication fork DNA, replisome components and a ubiquitin threshold. eLife, 9:e60371. doi: 10.7554/eLife.60371. 2019 Sonneville, R., Bhowmick, R. Hoffmann, S., Mailand, N., Hickson, I.D., Labib, K. (2019) TRAIP drives replisome disassembly and mitotic DNA repair synthesis at sites of incomplete DNA replication. eLife, 8:e48686. Doi: 10.7554/eLife.48686. Mukherjee, P.P. and Labib, K.P.M. (2019) In Vitro Reconstitution Defines the Minimal Requirements for Cdc48-dependent Disassembly of the CMG helicase in Budding Yeast. Cell Rep., 28, 2777-2783. Deegan, T.D., Baxter, J., Ortiz-Bazan, M.A., Yeeles, T.P., Labib, K.P.M. (2019) Pif1-family helicases support fork convergence during DNA replication termination in eukaryotes. Mol. Cell, 18, 231-244. Deng L, Wu RA, Sonneville R, Kochenova OV, Labib K, Pellman D, Walter JC. (2019) Mitotic CDK Promotes Replisome Disassembly, Fork Breakage, and Complex DNA Rearrangements. Mol Cell, 73, 915-929. 2018 Evrin C, Maman JD, Diamante A, Pellegrini L, Labib K. (2018) Histone H2A-H2B binding by Pol α in the eukaryotic replisome contributes to the maintenance of repressive chromatin. EMBO J., 37, pii: e99021. doi: 10.15252/embj.201899021 Gan, H., Serra-Cardona, A., Hua, X, Zhou, H., Labib, K., Yu, C., Zhang, Z. (2018) The Mcm2-Ctf4-Polα axis facilitates parental histone H3-H4 transfer to lagging strands. Mol. Cell, 72, 140-151. Hong Y, Velkova M, Silva N, Jagut M, Scheidt V, Labib K, Jantsch V, Gartner A. (2018) The conserved LEM-3/Ankle1 nuclease is involved in the combinatorial regulation of meiotic recombination repair and chromosome segregation in Caenorhabditis elegans. PLoS Genet. 14(6):e1007453. doi: 10.1371/journal.pgen.1007453. Hong, Y., Sonneville, R., Wang, B., Scheidt, V., Meier, B., Woglar, A., Demetriou, S., Labib, K., Jantsch, V., and Gartner, A. (2018) LEM-3 is a midbody-tethered nuclease that resolves chromatin bridges during late mitosis. Nat. Commun. 9, 728. doi: 10.1038/s41467-018-03135-w. 2017 Wu Y., Villa F., Maman J., Dobnikar L., Lau Y.H., Simon A.C., Labib K., Spring D.R. and Pellegrini L. (2017) Targeting the genome stability hub Ctf4 by stapled peptide design. Angew Chem Int Ed Engl. doi: 10.1002/anie.201705611. Sonneville R, Moreno SP, Knebel A, Johnson C, Hastie CJ, Gartner A, Gambus A, Labib K. (2017) CUL-2LRR-1 and UBXN-3 drive replisome disassembly during DNA replication termination and mitosis. Nat Cell Biol. 19, 468-479. Maric M, Mukherjee P, Tatham MH, Hay R, Labib K. (2017) Ufd1-Npl4 Recruit Cdc48 for Disassembly of Ubiquitylated CMG Helicase at the End of Chromosome Replication. Cell Rep. 18, 3033-3042. 2016 Villa, F., Simon, A. C., Ortiz-Bazan, M. A., Kilkenny, M. L., Wirthensohn, D., Wightman, M., Matak-Vinkovic, D., Pellegrini, L. and Labib, K. (2016) Ctf4 is a hub in the eukaryotic replisome that links multiple CIP-box proteins to the CMG helicase. Mol. Cell, 63, 1-12. Abdul Rehman, S. A., Kristariyanto, Y. A., Choi, S-Y., Nkosi, P. J., Weidlich, S., Labib, K., Hofmann, K. and Kulathu, Y. (2016) MINDY-1 Is a Member of an Evolutionarily Conserved and Structurally Distinct New Family of Deubiquitinating Enzymes. Mol. Cell, 63, 146-155. Bell, S. P. and Labib, K. (2016) Chromosome Duplication in Saccharomyces cerevisiae. Genetics, 203, 1027-1067.. Buser, R., Kellner, V., Melnik, A., Wilson-Zbinden, C., Schellhaas, R., Kastner, L., Piwko, W., Dees, M., Picotti, P., Maric, M., Labib, K., Luke, B. and Peter, M. (2016) The replisome-coupled E3 ubiquitin ligase Rtt101Mms22 counteracts Mrc1 function to tolerate genotoxic stress. PLOS Genetics, DOI:10.1371/journal.pgen.1005843. 2015 Maculins, T., Nkosi, P. J., Nishikawa, H. and Labib, K. (2015). Tethering of SCF to the Replisome Promotes Efficient Ubiquitylation and Disassembly of the CMG Helicase. Curr. Biol., 25, 2254-2259. Garcia-Rodriguez, L. J., De Piccoli, G., Marchesi, V., Jones, R. C., Edmondson, R. D. and Labib, K. (2015). A conserved Pol epsilon binding module in Ctf18-RFC is required for S-phase checkpoint activation downstream of Mec1. Nucleic Acids Res., 43, 8830-8838. Sonneville, R., Craig, G., Labib, K., Gartner, A. and Blow, J. J. (2015). Both Chromosome Decondensation and Condensation Are Dependent on DNA Replication in C. elegans Embryos. Cell Rep. 12, 405-417. 2014 Maric, M., Maculins, T., De Piccoli, G. and Labib, K. (2014). Cdc48 and a ubiquitin ligase drive disassembly of the CMG helicase at the end of DNA replication. Science, 346 (6208):1253596. Doi: 10.1126/science.1253596. Simon, A. C., Zhou, J. C., Perera, R. L., van Deursen, F., Evrin, C., Ivanova, M. E., Kilkenny, M. L., Renault, L., Kjaer, S., Matak-Vinkovic, D., Labib, K., Costa, A. and Pellegrini, L. (2014). A Ctf4 trimer couples the CMG helicase to DNA polymerase alpha in the eukaryotic replisome. Nature, 510, pp. 293-297. 2013 Foltman, M., Evrin, C., De Piccoli, G., Jones, R.C., Edmondson, R.D., Katou, Y., Nakato, R., Shirahige, K. and Labib, K. (2013) Eukaryotic replisome components cooperate to process histones during chromosome replication. Cell Reports, 3, 892-904. Sengupta, S., van Deursen, F., De Piccoli, G. and Labib, K. (2013) Dpb2 integrates the leading strand DNA polymerase into the eukaryotic replisome. Curr. Biol., 23, 543-552. Nkosi, P., Targosz, B.-S., Labib, K., Sanchez-Diaz, A. (2013) Hof1 and Rvs167 have redundant roles in actomyosin ring function during cytokinesis in budding yeast. PLOS ONE, 8, e57846. doi: 10.1371/journal.pone.0057846. 2012 De Piccoli, G., Katou, Y., Itoh, T., Nakato, R., Shirahige, K., and Labib, K. (2012) Replisome Stability at Defective DNA Replication Forks Is Independent of S Phase Checkpoint Kinases. Mol. Cell, 45, 696-704. Devrekanli, A., Foltman, M., Roncero, C., Sanchez-Diaz, A. and Labib, K. (2012) Inn1 and Cyk3 regulate chitin synthase during cytokinesis in budding yeasts. J. Cell Sci., 125, 5453-5466. Sanchez-Diaz, A., Nkosi, P., Murray, S. and Labib, K. (2012) The Mitotic Exit Network and Cdc14 phosphatase initiate cytokinesis by counteracting CDK phosphorylations and blocking polarised growth. EMBO J., 31, 3620-3634. Kilkenny, M.L., De Piccoli, G., Perera, R.L., Labib, K.* and Pellegrini, L.* (2012) A conserved motif in the C-terminal tail of DNA polymerase α tethers primase to the eukaryotic replisome. J. Biol. Chem., 287, 23740-23747. *co-corresponding authors van Deursen F, Sengupta S, De Piccoli G, Sanchez-Diaz A, Labib K. (2012) Mcm10 associates with the loaded DNA helicase at replication origins and defines a novel step in its activation. EMBO J., 31, 2195-2206. 2011 Labib, K. (2011) Building a double hexamer of DNA helicase at eukaryotic replication origins. EMBO J., 30, 4853-4855. Labib, K. and De Piccoli, G. (2011) Surviving chromosome replication: the many roles of the S-phase checkpoint pathway. Phil. Trans. Roy. Soc. B, 366, 3554-3561. 2010 Labib, K. (2010) How do Cdc7 and cyclin dependent kinases trigger the initiation of chromosome replication in eukaryotic cells? Genes Dev., 24, 1208-1219. 2009 Morohashi, H., Maculins, T., Labib, K. (2009) The amino terminal TPR domain of Dia2 connects SCFDia2 to the Replisome Progression Complex. Curr. Biol., 19, 1943-1949. Gambus, A., van Deursen, F., Polychronopoulos D, Foltman M, Jones RC, Edmondson RD, Calzada A, Labib K. (2009) A key role for Ctf4 in coupling the MCM2-7 helicase to DNA polymerase alpha within the eukaryotic replisome. EMBO J., 28, 2992-3004. 2008 Sanchez-Diaz A, Marchesi V, Murray S, Jones R, Pereira G, Edmondson R, Allen T, Labib K. (2008) Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast. Nat. Cell Biol., 10, 395-406. De la Cueva-Mendez, G. and Labib, K. (2008) New insights into the chromosome cycle. EMBO Rep., 9, 1177-81. Labib, K. (2008) Labib, K. (2008) Making connections at DNA replication forks: Mrc1 takes the lead. Mol. Cell, 32 ,166-8. 2007 Hodgson, B., Calzada, A. and Labib, K. (2007) Mrc1 and Tof1 regulate DNA replication forks in different ways during normal S phase. Mol. Biol. Cell (18), 3894-3902. Labib, K., and Gambus, A. (2007) A key role for the GINS complex at DNA replication forks. Trends Cell Biol. 17, 271-278. Labib, K. (2007) Highlights of the 23rd Genes and Cancer meeting University of Warwick 11-13 December 2006. Oncogene, 26, 2675-2677 Labib, K. and Hodgson, B. (2007) Replication fork barriers: pausing for a break or stalling for time? EMBO Rep., 8, 346-353. 2006 Kanemaki, M. and Labib, K. (2006) Distinct roles for Sld3 and GINS during establishment and progression of eukaryotic DNA replication forks. EMBO J., 25, 1753-1763. Gambus, A., Jones, R.C., Sanchez-Diaz, A., Kanemaki, M., van Deursen, F., Edmondson, R.D., and Labib, K. (2006) GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forks. Nat. Cell Biol., 8, 358-366. Schwob, E. and Labib, K. (2006) Regulating Initiation events in Yeasts. In DNA replication and human disease (ed. M.L. DePamphilis), pp. 295-312. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York . 2005 Calzada, A., Hodgson, B., Kanemaki, M., Bueno, A. and Labib, K. (2005). Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication fork. Genes Dev., 19, 1905-1919. 2004 A. Sanchez-Diaz, M. Kanemaki, V. Marchesi, K. Labib (2004). Rapid Depletion of Budding Yeast Proteins by Fusion to a Heat-Inducible Degron. Sci. STKE 2004, pl8. 2003 Kanemaki, M, Sanchez-Diaz, A., Gambus, A. and Labib, K. (2003) Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo. Nature, 423, 720-724. 2002 Diffley, J.F.X. and Labib, K. (2002) The Chromosome Replication Cycle. J.Cell Sci. 115, 869-872.

Home

Karim Labib

Research

Publications

The Group

Where are we?

Contact

View all Karim's publications via PubMed

2020 Deegan, T.D., Mukherjee, P.P., Fujisawa, R., Polo Rivera, C., Labib, K.P.M. (2020) CMG disassembly is controlled by replication fork DNA, replisome components and a ubiquitin threshold. eLife, 9:e60371. doi: 10.7554/eLife.60371. 2019 Sonneville, R., Bhowmick, R. Hoffmann, S., Mailand, N., Hickson, I.D., Labib, K. (2019) TRAIP drives replisome disassembly and mitotic DNA repair synthesis at sites of incomplete DNA replication. eLife, 8:e48686. Doi: 10.7554/eLife.48686. Mukherjee, P.P. and Labib, K.P.M. (2019) In Vitro Reconstitution Defines the Minimal Requirements for Cdc48-dependent Disassembly of the CMG helicase in Budding Yeast. Cell Rep., 28, 2777-2783. Deegan, T.D., Baxter, J., Ortiz-Bazan, M.A., Yeeles, T.P., Labib, K.P.M. (2019) Pif1-family helicases support fork convergence during DNA replication termination in eukaryotes. Mol. Cell, 18, 231-244. Deng L, Wu RA, Sonneville R, Kochenova OV, Labib K, Pellman D, Walter JC. (2019) Mitotic CDK Promotes Replisome Disassembly, Fork Breakage, and Complex DNA Rearrangements. Mol Cell, 73, 915-929. 2018 Evrin C, Maman JD, Diamante A, Pellegrini L, Labib K. (2018) Histone H2A-H2B binding by Pol α in the eukaryotic replisome contributes to the maintenance of repressive chromatin. EMBO J., 37, pii: e99021. doi: 10.15252/embj.201899021 Gan, H., Serra-Cardona, A., Hua, X, Zhou, H., Labib, K., Yu, C., Zhang, Z. (2018) The Mcm2-Ctf4-Polα axis facilitates parental histone H3-H4 transfer to lagging strands. Mol. Cell, 72, 140-151. Hong Y, Velkova M, Silva N, Jagut M, Scheidt V, Labib K, Jantsch V, Gartner A. (2018) The conserved LEM-3/Ankle1 nuclease is involved in the combinatorial regulation of meiotic recombination repair and chromosome segregation in Caenorhabditis elegans. PLoS Genet. 14(6):e1007453. doi: 10.1371/journal.pgen.1007453. Hong, Y., Sonneville, R., Wang, B., Scheidt, V., Meier, B., Woglar, A., Demetriou, S., Labib, K., Jantsch, V., and Gartner, A. (2018) LEM-3 is a midbody-tethered nuclease that resolves chromatin bridges during late mitosis. Nat. Commun. 9, 728. doi: 10.1038/s41467-018-03135-w. 2017 Wu Y., Villa F., Maman J., Dobnikar L., Lau Y.H., Simon A.C., Labib K., Spring D.R. and Pellegrini L. (2017) Targeting the genome stability hub Ctf4 by stapled peptide design. Angew Chem Int Ed Engl. doi: 10.1002/anie.201705611. Sonneville R, Moreno SP, Knebel A, Johnson C, Hastie CJ, Gartner A, Gambus A, Labib K. (2017) CUL-2LRR-1 and UBXN-3 drive replisome disassembly during DNA replication termination and mitosis. Nat Cell Biol. 19, 468-479. Maric M, Mukherjee P, Tatham MH, Hay R, Labib K. (2017) Ufd1-Npl4 Recruit Cdc48 for Disassembly of Ubiquitylated CMG Helicase at the End of Chromosome Replication. Cell Rep. 18, 3033-3042. 2016 Villa, F., Simon, A. C., Ortiz-Bazan, M. A., Kilkenny, M. L., Wirthensohn, D., Wightman, M., Matak-Vinkovic, D., Pellegrini, L. and Labib, K. (2016) Ctf4 is a hub in the eukaryotic replisome that links multiple CIP-box proteins to the CMG helicase. Mol. Cell, 63, 1-12. Abdul Rehman, S. A., Kristariyanto, Y. A., Choi, S-Y., Nkosi, P. J., Weidlich, S., Labib, K., Hofmann, K. and Kulathu, Y. (2016) MINDY-1 Is a Member of an Evolutionarily Conserved and Structurally Distinct New Family of Deubiquitinating Enzymes. Mol. Cell, 63, 146-155. Bell, S. P. and Labib, K. (2016) Chromosome Duplication in Saccharomyces cerevisiae. Genetics, 203, 1027-1067.. Buser, R., Kellner, V., Melnik, A., Wilson-Zbinden, C., Schellhaas, R., Kastner, L., Piwko, W., Dees, M., Picotti, P., Maric, M., Labib, K., Luke, B. and Peter, M. (2016) The replisome-coupled E3 ubiquitin ligase Rtt101Mms22 counteracts Mrc1 function to tolerate genotoxic stress. PLOS Genetics, DOI:10.1371/journal.pgen.1005843. 2015 Maculins, T., Nkosi, P. J., Nishikawa, H. and Labib, K. (2015). Tethering of SCF to the Replisome Promotes Efficient Ubiquitylation and Disassembly of the CMG Helicase. Curr. Biol., 25, 2254-2259. Garcia-Rodriguez, L. J., De Piccoli, G., Marchesi, V., Jones, R. C., Edmondson, R. D. and Labib, K. (2015). A conserved Pol epsilon binding module in Ctf18-RFC is required for S-phase checkpoint activation downstream of Mec1. Nucleic Acids Res., 43, 8830-8838. Sonneville, R., Craig, G., Labib, K., Gartner, A. and Blow, J. J. (2015). Both Chromosome Decondensation and Condensation Are Dependent on DNA Replication in C. elegans Embryos. Cell Rep. 12, 405-417. 2014 Maric, M., Maculins, T., De Piccoli, G. and Labib, K. (2014). Cdc48 and a ubiquitin ligase drive disassembly of the CMG helicase at the end of DNA replication. Science, 346 (6208):1253596. Doi: 10.1126/science.1253596. Simon, A. C., Zhou, J. C., Perera, R. L., van Deursen, F., Evrin, C., Ivanova, M. E., Kilkenny, M. L., Renault, L., Kjaer, S., Matak-Vinkovic, D., Labib, K., Costa, A. and Pellegrini, L. (2014). A Ctf4 trimer couples the CMG helicase to DNA polymerase alpha in the eukaryotic replisome. Nature, 510, pp. 293-297. 2013 Foltman, M., Evrin, C., De Piccoli, G., Jones, R.C., Edmondson, R.D., Katou, Y., Nakato, R., Shirahige, K. and Labib, K. (2013) Eukaryotic replisome components cooperate to process histones during chromosome replication. Cell Reports, 3, 892-904. Sengupta, S., van Deursen, F., De Piccoli, G. and Labib, K. (2013) Dpb2 integrates the leading strand DNA polymerase into the eukaryotic replisome. Curr. Biol., 23, 543-552. Nkosi, P., Targosz, B.-S., Labib, K., Sanchez-Diaz, A. (2013) Hof1 and Rvs167 have redundant roles in actomyosin ring function during cytokinesis in budding yeast. PLOS ONE, 8, e57846. doi: 10.1371/journal.pone.0057846. 2012 De Piccoli, G., Katou, Y., Itoh, T., Nakato, R., Shirahige, K., and Labib, K. (2012) Replisome Stability at Defective DNA Replication Forks Is Independent of S Phase Checkpoint Kinases. Mol. Cell, 45, 696-704. Devrekanli, A., Foltman, M., Roncero, C., Sanchez-Diaz, A. and Labib, K. (2012) Inn1 and Cyk3 regulate chitin synthase during cytokinesis in budding yeasts. J. Cell Sci., 125, 5453-5466. Sanchez-Diaz, A., Nkosi, P., Murray, S. and Labib, K. (2012) The Mitotic Exit Network and Cdc14 phosphatase initiate cytokinesis by counteracting CDK phosphorylations and blocking polarised growth. EMBO J., 31, 3620-3634. Kilkenny, M.L., De Piccoli, G., Perera, R.L., Labib, K.* and Pellegrini, L.* (2012) A conserved motif in the C-terminal tail of DNA polymerase α tethers primase to the eukaryotic replisome. J. Biol. Chem., 287, 23740-23747. *co-corresponding authors van Deursen F, Sengupta S, De Piccoli G, Sanchez-Diaz A, Labib K. (2012) Mcm10 associates with the loaded DNA helicase at replication origins and defines a novel step in its activation. EMBO J., 31, 2195-2206. 2011 Labib, K. (2011) Building a double hexamer of DNA helicase at eukaryotic replication origins. EMBO J., 30, 4853-4855. Labib, K. and De Piccoli, G. (2011) Surviving chromosome replication: the many roles of the S-phase checkpoint pathway. Phil. Trans. Roy. Soc. B, 366, 3554-3561. 2010 Labib, K. (2010) How do Cdc7 and cyclin dependent kinases trigger the initiation of chromosome replication in eukaryotic cells? Genes Dev., 24, 1208-1219. 2009 Morohashi, H., Maculins, T., Labib, K. (2009) The amino terminal TPR domain of Dia2 connects SCFDia2 to the Replisome Progression Complex. Curr. Biol., 19, 1943-1949. Gambus, A., van Deursen, F., Polychronopoulos D, Foltman M, Jones RC, Edmondson RD, Calzada A, Labib K. (2009) A key role for Ctf4 in coupling the MCM2-7 helicase to DNA polymerase alpha within the eukaryotic replisome. EMBO J., 28, 2992-3004. 2008 Sanchez-Diaz A, Marchesi V, Murray S, Jones R, Pereira G, Edmondson R, Allen T, Labib K. (2008) Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast. Nat. Cell Biol., 10, 395-406. De la Cueva-Mendez, G. and Labib, K. (2008) New insights into the chromosome cycle. EMBO Rep., 9, 1177-81. Labib, K. (2008) Labib, K. (2008) Making connections at DNA replication forks: Mrc1 takes the lead. Mol. Cell, 32 ,166-8. 2007 Hodgson, B., Calzada, A. and Labib, K. (2007) Mrc1 and Tof1 regulate DNA replication forks in different ways during normal S phase. Mol. Biol. Cell (18), 3894-3902. Labib, K., and Gambus, A. (2007) A key role for the GINS complex at DNA replication forks. Trends Cell Biol. 17, 271-278. Labib, K. (2007) Highlights of the 23rd Genes and Cancer meeting University of Warwick 11-13 December 2006. Oncogene, 26, 2675-2677 Labib, K. and Hodgson, B. (2007) Replication fork barriers: pausing for a break or stalling for time? EMBO Rep., 8, 346-353. 2006 Kanemaki, M. and Labib, K. (2006) Distinct roles for Sld3 and GINS during establishment and progression of eukaryotic DNA replication forks. EMBO J., 25, 1753-1763. Gambus, A., Jones, R.C., Sanchez-Diaz, A., Kanemaki, M., van Deursen, F., Edmondson, R.D., and Labib, K. (2006) GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forks. Nat. Cell Biol., 8, 358-366. Schwob, E. and Labib, K. (2006) Regulating Initiation events in Yeasts. In DNA replication and human disease (ed. M.L. DePamphilis), pp. 295-312. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York . 2005 Calzada, A., Hodgson, B., Kanemaki, M., Bueno, A. and Labib, K. (2005). Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication fork. Genes Dev., 19, 1905-1919. 2004 A. Sanchez-Diaz, M. Kanemaki, V. Marchesi, K. Labib (2004). Rapid Depletion of Budding Yeast Proteins by Fusion to a Heat-Inducible Degron. Sci. STKE 2004, pl8. 2003 Kanemaki, M, Sanchez-Diaz, A., Gambus, A. and Labib, K. (2003) Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo. Nature, 423, 720-724. 2002 Diffley, J.F.X. and Labib, K. (2002) The Chromosome Replication Cycle. J.Cell Sci. 115, 869-872.

View all Karim's publications via PubMed

2020 Deegan, T.D., Mukherjee, P.P., Fujisawa, R., Polo Rivera, C., Labib, K.P.M. (2020) CMG disassembly is controlled by replication fork DNA, replisome components and a ubiquitin threshold. eLife, 9:e60371. doi: 10.7554/eLife.60371. 2019 Sonneville, R., Bhowmick, R. Hoffmann, S., Mailand, N., Hickson, I.D., Labib, K. (2019) TRAIP drives replisome disassembly and mitotic DNA repair synthesis at sites of incomplete DNA replication. eLife, 8:e48686. Doi: 10.7554/eLife.48686. Mukherjee, P.P. and Labib, K.P.M. (2019) In Vitro Reconstitution Defines the Minimal Requirements for Cdc48-dependent Disassembly of the CMG helicase in Budding Yeast. Cell Rep., 28, 2777-2783. Deegan, T.D., Baxter, J., Ortiz-Bazan, M.A., Yeeles, T.P., Labib, K.P.M. (2019) Pif1-family helicases support fork convergence during DNA replication termination in eukaryotes. Mol. Cell, 18, 231-244. Deng L, Wu RA, Sonneville R, Kochenova OV, Labib K, Pellman D, Walter JC. (2019) Mitotic CDK Promotes Replisome Disassembly, Fork Breakage, and Complex DNA Rearrangements. Mol Cell, 73, 915-929. 2018 Evrin C, Maman JD, Diamante A, Pellegrini L, Labib K. (2018) Histone H2A-H2B binding by Pol α in the eukaryotic replisome contributes to the maintenance of repressive chromatin. EMBO J., 37, pii: e99021. doi: 10.15252/embj.201899021 Gan, H., Serra-Cardona, A., Hua, X, Zhou, H., Labib, K., Yu, C., Zhang, Z. (2018) The Mcm2-Ctf4-Polα axis facilitates parental histone H3-H4 transfer to lagging strands. Mol. Cell, 72, 140-151. Hong Y, Velkova M, Silva N, Jagut M, Scheidt V, Labib K, Jantsch V, Gartner A. (2018) The conserved LEM-3/Ankle1 nuclease is involved in the combinatorial regulation of meiotic recombination repair and chromosome segregation in Caenorhabditis elegans. PLoS Genet. 14(6):e1007453. doi: 10.1371/journal.pgen.1007453. Hong, Y., Sonneville, R., Wang, B., Scheidt, V., Meier, B., Woglar, A., Demetriou, S., Labib, K., Jantsch, V., and Gartner, A. (2018) LEM-3 is a midbody-tethered nuclease that resolves chromatin bridges during late mitosis. Nat. Commun. 9, 728. doi: 10.1038/s41467-018-03135-w. 2017 Wu Y., Villa F., Maman J., Dobnikar L., Lau Y.H., Simon A.C., Labib K., Spring D.R. and Pellegrini L. (2017) Targeting the genome stability hub Ctf4 by stapled peptide design. Angew Chem Int Ed Engl. doi: 10.1002/anie.201705611. Sonneville R, Moreno SP, Knebel A, Johnson C, Hastie CJ, Gartner A, Gambus A, Labib K. (2017) CUL-2LRR-1 and UBXN-3 drive replisome disassembly during DNA replication termination and mitosis. Nat Cell Biol. 19, 468-479. Maric M, Mukherjee P, Tatham MH, Hay R, Labib K. (2017) Ufd1-Npl4 Recruit Cdc48 for Disassembly of Ubiquitylated CMG Helicase at the End of Chromosome Replication. Cell Rep. 18, 3033-3042. 2016 Villa, F., Simon, A. C., Ortiz-Bazan, M. A., Kilkenny, M. L., Wirthensohn, D., Wightman, M., Matak-Vinkovic, D., Pellegrini, L. and Labib, K. (2016) Ctf4 is a hub in the eukaryotic replisome that links multiple CIP-box proteins to the CMG helicase. Mol. Cell, 63, 1-12. Abdul Rehman, S. A., Kristariyanto, Y. A., Choi, S-Y., Nkosi, P. J., Weidlich, S., Labib, K., Hofmann, K. and Kulathu, Y. (2016) MINDY-1 Is a Member of an Evolutionarily Conserved and Structurally Distinct New Family of Deubiquitinating Enzymes. Mol. Cell, 63, 146-155. Bell, S. P. and Labib, K. (2016) Chromosome Duplication in Saccharomyces cerevisiae. Genetics, 203, 1027-1067.. Buser, R., Kellner, V., Melnik, A., Wilson-Zbinden, C., Schellhaas, R., Kastner, L., Piwko, W., Dees, M., Picotti, P., Maric, M., Labib, K., Luke, B. and Peter, M. (2016) The replisome-coupled E3 ubiquitin ligase Rtt101Mms22 counteracts Mrc1 function to tolerate genotoxic stress. PLOS Genetics, DOI:10.1371/journal.pgen.1005843. 2015 Maculins, T., Nkosi, P. J., Nishikawa, H. and Labib, K. (2015). Tethering of SCF to the Replisome Promotes Efficient Ubiquitylation and Disassembly of the CMG Helicase. Curr. Biol., 25, 2254-2259. Garcia-Rodriguez, L. J., De Piccoli, G., Marchesi, V., Jones, R. C., Edmondson, R. D. and Labib, K. (2015). A conserved Pol epsilon binding module in Ctf18-RFC is required for S-phase checkpoint activation downstream of Mec1. Nucleic Acids Res., 43, 8830-8838. Sonneville, R., Craig, G., Labib, K., Gartner, A. and Blow, J. J. (2015). Both Chromosome Decondensation and Condensation Are Dependent on DNA Replication in C. elegans Embryos. Cell Rep. 12, 405-417. 2014 Maric, M., Maculins, T., De Piccoli, G. and Labib, K. (2014). Cdc48 and a ubiquitin ligase drive disassembly of the CMG helicase at the end of DNA replication. Science, 346 (6208):1253596. Doi: 10.1126/science.1253596. Simon, A. C., Zhou, J. C., Perera, R. L., van Deursen, F., Evrin, C., Ivanova, M. E., Kilkenny, M. L., Renault, L., Kjaer, S., Matak-Vinkovic, D., Labib, K., Costa, A. and Pellegrini, L. (2014). A Ctf4 trimer couples the CMG helicase to DNA polymerase alpha in the eukaryotic replisome. Nature, 510, pp. 293-297. 2013 Foltman, M., Evrin, C., De Piccoli, G., Jones, R.C., Edmondson, R.D., Katou, Y., Nakato, R., Shirahige, K. and Labib, K. (2013) Eukaryotic replisome components cooperate to process histones during chromosome replication. Cell Reports, 3, 892-904. Sengupta, S., van Deursen, F., De Piccoli, G. and Labib, K. (2013) Dpb2 integrates the leading strand DNA polymerase into the eukaryotic replisome. Curr. Biol., 23, 543-552. Nkosi, P., Targosz, B.-S., Labib, K., Sanchez-Diaz, A. (2013) Hof1 and Rvs167 have redundant roles in actomyosin ring function during cytokinesis in budding yeast. PLOS ONE, 8, e57846. doi: 10.1371/journal.pone.0057846. 2012 De Piccoli, G., Katou, Y., Itoh, T., Nakato, R., Shirahige, K., and Labib, K. (2012) Replisome Stability at Defective DNA Replication Forks Is Independent of S Phase Checkpoint Kinases. Mol. Cell, 45, 696-704. Devrekanli, A., Foltman, M., Roncero, C., Sanchez-Diaz, A. and Labib, K. (2012) Inn1 and Cyk3 regulate chitin synthase during cytokinesis in budding yeasts. J. Cell Sci., 125, 5453-5466. Sanchez-Diaz, A., Nkosi, P., Murray, S. and Labib, K. (2012) The Mitotic Exit Network and Cdc14 phosphatase initiate cytokinesis by counteracting CDK phosphorylations and blocking polarised growth. EMBO J., 31, 3620-3634. Kilkenny, M.L., De Piccoli, G., Perera, R.L., Labib, K.* and Pellegrini, L.* (2012) A conserved motif in the C-terminal tail of DNA polymerase α tethers primase to the eukaryotic replisome. J. Biol. Chem., 287, 23740-23747. *co-corresponding authors van Deursen F, Sengupta S, De Piccoli G, Sanchez-Diaz A, Labib K. (2012) Mcm10 associates with the loaded DNA helicase at replication origins and defines a novel step in its activation. EMBO J., 31, 2195-2206. 2011 Labib, K. (2011) Building a double hexamer of DNA helicase at eukaryotic replication origins. EMBO J., 30, 4853-4855. Labib, K. and De Piccoli, G. (2011) Surviving chromosome replication: the many roles of the S-phase checkpoint pathway. Phil. Trans. Roy. Soc. B, 366, 3554-3561. 2010 Labib, K. (2010) How do Cdc7 and cyclin dependent kinases trigger the initiation of chromosome replication in eukaryotic cells? Genes Dev., 24, 1208-1219. 2009 Morohashi, H., Maculins, T., Labib, K. (2009) The amino terminal TPR domain of Dia2 connects SCFDia2 to the Replisome Progression Complex. Curr. Biol., 19, 1943-1949. Gambus, A., van Deursen, F., Polychronopoulos D, Foltman M, Jones RC, Edmondson RD, Calzada A, Labib K. (2009) A key role for Ctf4 in coupling the MCM2-7 helicase to DNA polymerase alpha within the eukaryotic replisome. EMBO J., 28, 2992-3004. 2008 Sanchez-Diaz A, Marchesi V, Murray S, Jones R, Pereira G, Edmondson R, Allen T, Labib K. (2008) Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast. Nat. Cell Biol., 10, 395-406. De la Cueva-Mendez, G. and Labib, K. (2008) New insights into the chromosome cycle. EMBO Rep., 9, 1177-81. Labib, K. (2008) Labib, K. (2008) Making connections at DNA replication forks: Mrc1 takes the lead. Mol. Cell, 32 ,166-8. 2007 Hodgson, B., Calzada, A. and Labib, K. (2007) Mrc1 and Tof1 regulate DNA replication forks in different ways during normal S phase. Mol. Biol. Cell (18), 3894-3902. Labib, K., and Gambus, A. (2007) A key role for the GINS complex at DNA replication forks. Trends Cell Biol. 17, 271-278. Labib, K. (2007) Highlights of the 23rd Genes and Cancer meeting University of Warwick 11-13 December 2006. Oncogene, 26, 2675-2677 Labib, K. and Hodgson, B. (2007) Replication fork barriers: pausing for a break or stalling for time? EMBO Rep., 8, 346-353. 2006 Kanemaki, M. and Labib, K. (2006) Distinct roles for Sld3 and GINS during establishment and progression of eukaryotic DNA replication forks. EMBO J., 25, 1753-1763. Gambus, A., Jones, R.C., Sanchez-Diaz, A., Kanemaki, M., van Deursen, F., Edmondson, R.D., and Labib, K. (2006) GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forks. Nat. Cell Biol., 8, 358-366. Schwob, E. and Labib, K. (2006) Regulating Initiation events in Yeasts. In DNA replication and human disease (ed. M.L. DePamphilis), pp. 295-312. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York . 2005 Calzada, A., Hodgson, B., Kanemaki, M., Bueno, A. and Labib, K. (2005). Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication fork. Genes Dev., 19, 1905-1919. 2004 A. Sanchez-Diaz, M. Kanemaki, V. Marchesi, K. Labib (2004). Rapid Depletion of Budding Yeast Proteins by Fusion to a Heat-Inducible Degron. Sci. STKE 2004, pl8. 2003 Kanemaki, M, Sanchez-Diaz, A., Gambus, A. and Labib, K. (2003) Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo. Nature, 423, 720-724. 2002 Diffley, J.F.X. and Labib, K. (2002) The Chromosome Replication Cycle. J.Cell Sci. 115, 869-872.