Masato was the first member of the lab, joining in July 2001 after his PhD studies in Japan. Together with Alberto Sanchez-Diaz, Masato discovered components of the GINS complex and showed that they were required for elongation (Kanemaki et al, Nature, 2003), then showed that Sld3 is essential for initiation but is dispensable for fork progression (Kanemaki and Labib, EMBO J., 2006). Masato returned to Japan in 2006 and is now a tenured professor at the National Institute for Genetics in Mishima. Masato's group developed the 'auxin-inducible degron' method for conditional inactivation of proteins in animal or fungal cells (Nishimura et al, Nat. Methods, 2009; Natsume et al, Cell Rep., 2016).
Aga studied at the Jagiellonian University in Krakow, before joining us in 2002. Aga made the important discovery that GINS associated with Mcm2-7 and Cdc45, together with a set of other factors , to form the Replisome Progression Complex (Gambus et al, Nat. Cell Biol., 2006). Aga then joined Julian Blow's group in Dundee as a post-doc, funded by a Sir Henry Wellcome Postdoctoral Fellowship, before receiving MRC and Birmingham Fellowships to establish her own group at the University of Birmingham. Aga's group showed that ubiquitin and p97/Cdc48 are important for CMG helicase disassembly in Xenopus (Priego Moreno et al, Science, 2014), and in 2015 Aga received the Lister Research Prize and the BACR/AstraZeneca Young Investigator Award.
Alberto Sanchez Diaz
Alberto was a PhD student in Salamanca with Sergio Moreno and studied the regulation of the fission yeast cell cycle. After joining our group, Alberto used the heat-inducible degron to screen for new cell cycle proteins (Kanemaki, Sanchez-Diaz et al, Nature, 2003) and then focussed on a novel cytokinesis factor called Inn1 (Sanchez-Diaz et al, Nat. Cell Biol., 2009). Alberto returned to Spain with a Ramon y Cajal fellowship, setting up his own group at the University of Cantabria in Santander, where he is now a principal investigator. Alberto's group continue to study the mechanisms of cell division in budding yeast (Foltman et al, PLoS Genet., 2016).
Giacomo de Piccoli
Giacomo studied in Turin and was then a PhD student with Luis Aragon in London, studying the role of the SMC5-6 complex in genome stability. After joining our group, Giacomo found that the S-phase checkpoint regulates the function but not the stability of the replisome (De Piccoli et al, Mol. Cell, 2012). Giacomo was awarded a Career Development Fellowship by Cancer Research U.K. and set up his own lab at the University of Warwick in 2013. Giacomo's group now study the regulation of the replisome by sumoylation and the S-phase checkpoint, in response to defects in chromosome replication.
Sugopa studied bacterial gyrase enzymes during her PhD at the Indian Institute of Science in Bangalore, before joining our group in 2008. Sugopa showed that DNA polymerase epsilon is incorporated into the replisome by direct binding to the CMG helicase (Sugopa et al, Curr. Biol., 2013). Sugopa now runs her own group and is an assistant professor at the Presidency University in Kolkata.
Magda studied at the Jagiellonian University in Krakow, before joining us in 2006. Magda studied how the replisome contributes to processing of histones at DNA replication forks, and showed that the Mcm2 helicase subunit has a conserved histone-binding motif that is important to preserve repressive chromatin near telomeres (Foltman et al, Cell Rep., 2013). Magda is now a an assistant professor at the University of Santander in Spain, where she teaches and studies the regulation of cytokinesis (Foltman et al, PLoS Genetics, 2016).
Arturo studied the regulation of Cdc6 during his PhD with Avelino Bueno in Salamanca. Arturo then joined us for a year in Manchester, during which time he developed a system with which to study the regulation of paused replication forks in vivo. This work continued as a collaboration after Arturo returned to Salamanca (Calzada et al, Genes Dev., 2005; Hodgson et al, Mol. Biol. Cell, 2007). Arturo is now an investigator of the Spanish research council (CSIC), and runs his group at the Centro Nacional de Biotecnología in Madrid, where they study the links between DNA replication and genome integrity.
Asli Sadli (formerly Devrekanli)
Asli studied at Ankara University and joined us as a PhD student in 2007. She identifed the Inn1 protein as a key regulator of the chitin synthase Chs2 during cytokinesis (Devrekanli et al, J. Cell Sci., 2012). Asli returned to Turkey in 2012 and is now an assistant professor at Canik Basari University.
Marija studied in Zagreb, where she first heard of our lab at an EMBO 'Young Scientists Forum' that Karim helped to organise. Marija joined us in 2010 and studied the regulation of Mcm7 by ubiquitylation. This led to the discovery that Cdc48 is required for disassembly of ubiquitylated CMG helicase at the end of chromosome replication in budding yeast (Maric et al, Science, 2014). Marija moved with us to Dundee and stayed for a one-year post-doctoral period, before joining Simon Boulton's group at the Francis Crick institute in London.
Tim initially studied medicine in Latvia, but then switched to biomedical research and did a masters degree in Rotterdam in 2008. Tim joined us in 2009 and wanted to identify substrates of the ubiquitin ligase SCFDia2, which we had shown is associated with the Replisome Progressoin Complex (Morohashi et al, Curr. Biol., 2009). In collaboration with Marija Maric, Tim showed that SCFDia2 is required to ubiquitylated the CMG helicase in yeast cell extracts, and found that Dia2 is essential for CMG helicase disassembly (Maric, Maculins et al, Science, 2014; Maculins et al, Curr. Biol., 2015). Tim then joined Astrazeneca for two years as a post-doc (Maculins et al, Sci. Rep., 2016), and is currently a post-doctoral fellow in the group of Ivan Dikic in Frankfurt, supported by a fellowship from the Human Frontier Science Programme.
Kohei did his PhD in the laboratory of Masato Kanemaki at the National Institute of Genetics in Mishima, Japan, where he developed the 'auxin-inducible degron' method (Nishimura et al, Nat. Methods, 2009). Kohei received a fellowship from the Japan Society for the Promotion of Science to join us for a year in Dundee, where he established protocols for genome editing in mouse ES cells by CRISPR-Cas9. Kohei is now a post-doctoral fellow in the group of Tatsuo Fukugawa at the University of Osaka.
Hiroko Nishikawa (formerly Morohashi)
Hiroko studied at Osaka University and joined us as a PhD student in 2004. She showed that the E3 ubiquitin ligase SCFDia2 was associated with the Replisome Progression Complex (Morohashi et al, Curr. Biol., 2009). Hiroko returned to Japan and is now a Junior Fellow at the Max Plank-University of Tokyo Center for Integrative Inflammatology, and 'Project Research Associate' for the 'CREST' project.
Luis Javier Garcia Rodriguez
Luis studied cell division as a PhD student in Salamanca and a postdoc at Columbia University in New York, before joining us a post-doctoral fellow in 2008. Luis showed that Ctf18-RFC uses a motif conserved from humans to yeast to associate with DNA polymerase epsilon (Garcia Rodriguez et al, Nuc. Acids Res., 2015). Luis then moved to Dundee to join the group of Tomo Tanaka, where he studies aspects of chromosome segregation in budding yeast.
Frederick van Deursen
Principal Scientific Officer 2004-2013
Ricky joined the lab in Manchester as a 'Principal Scientific Officer', and studied the roles of Ctf4 (Gambus et al, EMBO J., 2009) and Mcm10 (van Deursen et al, EMBO J., 2012) during chromosome replication. When the group re-located to Dundee in 2013, Ricky joined Qiagen as a Senior Scientist, responsible for assay design and development.
PhD student 2002-2006
Vanessa studied at Glasgow University before joining us as a PhD student in Manchester. She carried out part of our screen for new cell cycle proteins, using the heat-inducible degron method (Sanchez-Diaz et al, Nat. Cell Biol., 2009), and then characterised the Ctf18-RFC complex, showing that it associates with DNA polymerase epsilon (Garcia-Rodriguez et al, Nucl. Acids Res., 2015). Vanessa then worked as a post-doctoral fellow with the biotech company Pharmacia in Milan, and currently works as a scientific editor in Switzerland with The European Society for Medical Oncology.
Ben was a PhD student in Dundee with Julian Blow (Hodgson et al, Curr. Biol., 2002), before joining us as a post-doc in 2002. Ben collaborated with Arturo Calzada to establish a system for studying the regulation and composition of paused replisomes in vivo (Calzada et al, Genes Dev., 2005; Hodgson et al, Mol. Biol. Cell, 2007). After leaving the lab, Ben worked with Thomas Helleday and Steve D. Bell in Oxford, before returning to Manchester as a Scientific Officer in the group of Iain Hagan, in the Cancer Research U.K. Manchester research institute.
Pedro Junior Nkosi
Senior Research Technician
Tel.: 01382 386411
Pedro obtained his Diploma of Technician in Biomedical Analysis from the Ecole Supérieure de la Santé and then worked as a medical technician in Lausanne. Pedro joined our lab in Manchester in 2009 and then moved with us to Dundee in 2013. Pedro contributed to several of our yeast projects, but also expanded his work to mouse ES cells. Pedro returned to Switzerland in 2016 to work as a research technician in the lab of Sophie Martin at the University of Lausanne.
Research Technician 2001-2003
Abi was our first research technician (or 'Scientific Officer') and helped with the early stages of our functional genomic screens for new cell cycle proteins in budding yeast. In her spare time Abi represented Wales at fly fishing and crown green bowling, and in 2003 Abi returned to North Wales to join a Medical Equipment company.
Maria Angeles Ortiz
Tel.: 01382 386411
Maria Angeles was a PhD student in Madrid with Jose Antonio Tercero at the Centro de Biología Molecular Severo Ochoa (CBMSO). Maria Angeles joined our lab in 2014 and was interested in how defects in termination impact upon the maintenance of genome integrity.
After a period of maternity leave, Maria Angeles returned to Spain in the autumn of 2017.
Visiting student 2007 and 2008
Bianca studied for a masters degree in Munich and joined us for the summers of 2007 and 2008. She worked with Alberto Sanchez-Diaz and identified a role for the SH3 protein Rvs167 during cytokinesis (Nkosi et al, PLOS One, 2013). Bianca subsequently returned to Munich to undertake her PhD research.
Visiting student 2015
Fabian studied in Tubingen and did research for his masters project at the Max Plank insitute of Biochemistry in Martinsried. Fabian spent 9 months with us in Dundee, optimising purification protocols for complexes of replication proteins. Fabian is currently a PhD student in Barcelona at the Institute for Research in Biomedicine .
Visiting student 2015
Bret studied in York before joining the PhD programme of the MRC PPU. Bret joined us in 2015 for the first rotation project of his PhD.
Anna Segarra Fas
PhD rotation student
Tel.: 01382 386411
Anna is from Spain but did her BSc studies at the HAN University in Nijmegen in the Netherlands. During her degree, Anna visited Dundee for a on-year internship in John Rouse's lab, and then returned to the PPU as a PhD student in January 2017. Anna spent 4 months in our lab, using CRISPR as a tool to study the mouse replisome. She is now pursuing her PhD studies in the group of Greg Findlay.