Professor of Quantitative Cell Biology
|Address:||College of Life Sciences, University of Dundee, Dundee|
Mitotic chromosome dynamics
During cell division, the two copies of a cell's genetic material are completely separated and delivered to a pair of new daughter cells. Proper chromosome segregation requires the formation of correct attachments between the genetic material, assembled into chromosomes, and ends of microtubules. Our research is focussed on establishing the molecular mechanisms that mediate and monitor the correct attachment of chromosomes to microtubule at a special structure known as the kinetochore. To achieve this, we make use of advanced tools for imaging the components of cell division, especially in living cells and specialised mass spectrometry methods to probe the molecular machinery of cell division. Recently, we used these tools to discover a new protein, Bod1, that plays a critical role in the formation of correct attachments between microtubules and chromosomes by modulating the activity of Aurora B protein kinase. We recently installed a newly developed OMX microscope and are using this system to probe the inner workings of the centromere and kinetochore of the mitotic chromosome.
Like many cell biology labs, we generate large sets of image data. To manage, analyse and understand this data, we, long with our collaborators, formed the Open Microscopy Environment Consortium. OME develops data specifications, file format translators and data management software for imaging applications. OME tools are used in thousands of labs around the world. For more info on OME, see the Consortium's web site.
OMERO is used to store and visualise data for this project. An example of the image data can be viewed in OMERO by clicking on the thumbnail.
Selected Recent Publications
Beck J, Maerki S, Posch M, Metzger T, Persaud A, Scheel H, Hofmann K, Rotin D, Pedrioli P, Swedlow JR, Peter M, Sumara I (2013). Ubiquitylation-dependent localization of PLK1 in mitosis. Nat Cell Biol. 15:430-439.
Moser SC, Bensaddek D, Ortmann B, Mudie S, Blow JJ, Lamond AI, Swedlow JR, Rocha S. (2013) PHD1 links cell cycle progression to metabolic state through hydroxylation of the centrosomal protein Cep192. Dev Cell. 26:381-392.
Porter IM, Schleicher K, Porter M, Swedlow JR*. Bod1 regulates protein phosphatase 2A at mitotic kinetochores. (2013) Nat Comms 4:2677.
Lagerstedt I, Moore WJ, Patwardhan A, Sanz-García E, Best C, Swedlow JR, Kleywegt GJ. (2013) Web-based visualisation and analysis of 3D electron-microscopy data from EMDB and PDB. J Struct Biol 184:173-181.
Marlow VL, Porter M, Hobley L, Kiley TB, Swedlow JR, Davidson FA, Stanley-Wall, NR. (2014) DegU~P manipulates cell fate differentiation in the Bacillus subtilis biofilm. J. Bacteriol. 196:16-27.
Allan, C., Burel, J. M., Moore, J., Blackburn, C., Linkert, M., Loynton, S., Macdonald, D., Moore, W. J., Neves, C., Patterson, A., Porter, M., Tarkowska, A., Loranger, B., Avondo, J., Lagerstedt, I., Lianas, L., Leo, S., Hands, K., Hay, R. T., Patwardhan, A., Best, C., Kleywegt, G. J., Zanetti, G. and Swedlow, J. R. (2012) OME Remote Objects (OMERO): flexible, model-driven data management for experimental biology. Nat Methods. 9, 245-253, doi. 10.1038/nmeth.1896, PMID 22373911 View Paper
Posch M, Khoudoli GA, Swift S, King EM, DeLuca JG, Swedlow JR (2010) Sds22 regulates aurora B activity and microtubule-kinetochore interactions at mitosis. JCB. 191:61-74 PMID: 20921135 View Paper
Linkert M, Rueden CT, Allan C, Burel J-M, Moore W, Patterson A, Loranger B, Moore J, Neves C, MacDonald D, Tarkowska A, Sticco C, Hill E, Rossner M, Eliceiri KW, Swedlow J.R. (2010) Metadata matters: access to image data in the real world. 189: 777-782. PMID: 20513764 View Paper
Jaqaman K, King EM, Amaro AC, Winter JR, Dorn JF, Elliott HL, Mchedlishvili N, McClelland SE, Porter IM, Posch M, Toso A, Danuser G, McAinsh AD, Meraldi P, Swedlow J.R. (2010) Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases. J. Cell Biol. 188:681-692. PMID: 20212316 View Paper
Swedlow, J.R., Goldberg, I.G., Eliceiri, K.W., the OME Consortium (2009) Bioimage informatics for experimental biology. Annu Rev Biophys. 38:327-346. PMID: 19416072 View Paper
Khoudoli, G.A., Gillespie, P.J., Stewart, G., Andersen, J.S., Swedlow, J.R., and Blow, J.J. (2008) Temporal profiling of the chromatin proteome reveals system-wide responses to replication inhibition. Curr. Biol. 18:838-43. PMID: 18514518 View Paper
Murray, J.M., Appleton, P.L., Swedlow, J.R., and Waters, J.C. (2007) Evaluating Performance in three-dimensional fluorescence microscopy. J Microsc. 228(3):390-405. PMID: 18645334 View Paper
Porter, I.M., McClelland, S., Khoudoli, G.A., Hunter, C., Andersen, J., McAinsh, A.D., Blow, J.J., and Swedlow, J.R. (2007) Bod1, a novel kinetochore protein required for chromosome biorientation. J Cell Biol. 179(2):187-97. PMID: 17938248 View Paper
Wilcock, A.C., Swedlow, J.R., and Storey, K.G. (2007) Mitotic spindle orientation distinguishes stem cell and terminal modes of neuron production in the early spinal cord. Development 134:1943-1954. PMID: 17470968 View Paper
Goldberg, I.G., Allan, C., Burel, J-M., Creager, D., Falconi, A., Hochheiser, H.S., Johnston, J., Mellenm, J., Sorger, P.K., and Swedlow, J.R. (2005) The Open Microscopy Environment (OME) data model and XML File: open tools for informatics and quantitative analysis in biological Imaging.Genome Biol. 6:R47. PMID: 15892875 View Paper
Andrews, P.D., Ovechkina, Y., Morrice, N., Wagenbach, M., Duncan, K., Wordeman, L., and Swedlow, J.R. (2004) Aurora B regulates MCAK at the mitotic centromere. Dev Cell 6:253-268. PMID: 14960279 View Paper