"Evolution of microbial interactions in the context of chronic disease "
Monday, December 6, 2021 - 13:00 to 14:00
Dr. Megan Bergkessel
Professor Deborah Hogan
Geisel School of Medicine at Dartmouth University, Hanover, USA
Damage in the lungs of individuals with cystic fibrosis (CF) is regionally heterogeneous, with more damage in the upper lobes than in lower lobes and within localized regions of damage within each lobe. In addition, CF lung function is also variable over time. In this presentation, we present data on the regional and temporal analyses of bacteria and fungi in the CF lung using a combination of molecular and culture-based analysis of sputum and lavage samples. Through these studies, we reveal known and unexpected differences in isolate phenotype within a species and use in vitro evolution assays combined with forward and reverse genetics to propose a model for which selective pressures drive changes in infection populations over time. Furthermore, we describe interactions that occur between divergently evolved types. For relevant pathways, we use machine learning methods to analyze the transcriptomes upon growth in vivo-like conditions to gain insight into pathway-pathway relationships. This presentation will focus on the bacteriumPseudomonas aeruginosawith brief description of parallel studies in the fungusCandida lusitaniae.